Hyaluronan and Fibrin Biomaterial As Scaffolds for Neuronal Differentiation of Adult Stem Cells Derived from Adipose Tissue and Skin

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2011 Nov 11

PMID 22072917

Citations 9

Authors

Chiara Gardin

Vincenzo Vindigni

Eriberto Bressan

Letizia Ferroni

Elisa Nalesso

Alessandro Della Puppa

Domenico dAvella

Diego Lops

Paolo Pinton

Barbara Zavan

Affiliations

Soon will be listed here.

Abstract

Recently, we have described a simple protocol to obtain an enriched culture of adult stem cells organized in neurospheres from two post-natal tissues: skin and adipose tissue. Due to their possible application in neuronal tissue regeneration, here we tested two kinds of scaffold well known in tissue engineering application: hyaluronan based membranes and fibrin-glue meshes. Neurospheres from skin and adipose tissue were seeded onto two scaffold types: hyaluronan based membrane and fibrin-glue meshes. Neurospheres were then induced to acquire a glial and neuronal-like phenotype. Gene expression, morphological feature and chromosomal imbalance (kariotype) were analyzed and compared. Adipose and skin derived neurospheres are able to grow well and to differentiate into glial/neuron cells without any chromosomal imbalance in both scaffolds. Adult cells are able to express typical cell surface markers such as S100; GFAP; nestin; βIII tubulin; CNPase. In summary, we have demonstrated that neurospheres isolated from skin and adipose tissues are able to differentiate in glial/neuron-like cells, without any chromosomal imbalance in two scaffold types, useful for tissue engineering application: hyaluronan based membrane and fibrin-glue meshes.

Citing Articles

PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.

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In Vitro Model for the Evaluation of Innovative Transcatheter Debridement Device (TDD): Pericardium-Based Scaffold and Stem Cells to Reproduce Calcificated Valves.

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Potential of Fibrin Glue and Mesenchymal Stem Cells (MSCs) to Regenerate Nerve Injuries: A Systematic Review.

Ortiz A, Fideles S, Pomini K, Bellini M, Pereira E, Reis C Cells. 2022; 11(2).

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Characterization of Dermal Stem Cells of Diabetic Patients.

Ferroni L, Gardin C, Paola L, Campo G, Cimaglia P, Bellin G Cells. 2019; 8(7).

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3-D Bioprinting of Neural Tissue for Applications in Cell Therapy and Drug Screening.

Thomas M, Willerth S Front Bioeng Biotechnol. 2017; 5:69.

PMID: 29204424 PMC: 5698280. DOI: 10.3389/fbioe.2017.00069.

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