Smooth Muscle Proteins from Hirschsprung's Disease Facilitates Stem Cell Differentiation

Overview

Journal Pediatr Surg Int

Specialties General Surgery
Pediatrics

Date 2011 Nov 4

PMID 22048648

Citations 3

Authors

Cornelia Irene Hagl

Sabine Heumuller

Markus Klotz

Ulrike Subotic

Lucas Wessel

Karl-Herbert Schafer

Affiliations

Soon will be listed here.

Abstract

Background And Aims: The transplantation of neural crest derived stem cells (NCSC's) is a potent alternative for the treatment of Hirschsprung's disease (HSCR). Cells to be transplanted should find an appropriate microenvironment to survive and differentiate. To investigate the quality of this microenvironment, effects of HSCR-smooth-muscle-protein extracts upon NCSC's were studied in vitro.

Methods: Postnatal human gut from children undergoing colonic resection due to HSCR was divided in segments. Smooth muscle was dissected and homogenized. Glial-cell-line-derived-neurotrophic-factor (GDNF) concentration was measured in the homogenates from the individual segment using ELISA. NCSC's were exposed to protein extracts derived from ganglionic and aganglionic HSCR segments, and their effect upon neurite outgrowth, survival and branching was evaluated.

Results: The amount of the factors varied considerably between the proximal and distal segments, and also from patient to patient. While extracts from proximal segments tended to have more prominent effects, all HSCR-muscle-protein extracts increased neuronal survival and network formation.

Conclusion: Muscle protein from aganglionic bowel supports the survival and outgrowth of NCSC's and is so an appropriate target for neural stem cell treatment.

Citing Articles

Degradation of intestinal mRNA: a matter of treatment.

Heumuller-Klug S, Sticht C, Kaiser K, Wink E, Hagl C, Wessel L World J Gastroenterol. 2015; 21(12):3499-508.

PMID: 25834314 PMC: 4375571. DOI: 10.3748/wjg.v21.i12.3499.

Prospective identification and culture of rat enteric neural stem cells (ENSCs).

Gao T, Chen H, Liu M, Ge W, Yin Q Cytotechnology. 2014; 68(3):509-14.

PMID: 25407731 PMC: 4846640. DOI: 10.1007/s10616-014-9803-3.

Maintenance of the enteric stem cell niche by bacterial lipopolysaccharides? Evidence and perspectives.

Schuster A, Klotz M, Schwab T, Di Liddo R, Bertalot T, Schrenk S J Cell Mol Med. 2014; 18(7):1429-43.

PMID: 24780093 PMC: 4124026. DOI: 10.1111/jcmm.12292.

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