Development of Wild Barley (Hordeum Chilense)-derived DArT Markers and Their Use into Genetic and Physical Mapping

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2011 Nov 4

PMID 22048641

Citations 10

Authors

C Rodriguez-Suarez

M J Gimenez

N Gutierrez

C M Avila

A Machado

E Huttner

M C Ramirez

A C Martin

A Castillo

A Kilian

A Martin

S G Atienza

Affiliations

Soon will be listed here.

Abstract

Diversity arrays technology (DArT) genomic libraries were developed from H. chilense accessions to support robust genotyping of this species and a novel crop comprising H. chilense genome (e.g., tritordeums). Over 11,000 DArT clones were obtained using two complexity reduction methods. A subset of 2,209 DArT markers was identified on the arrays containing these clones as polymorphic between parents and segregating in a population of 92 recombinant inbred lines (RIL) developed from the cross between H. chilense accessions H1 and H7. Using the segregation data a high-density map of 1,503 cM was constructed with average inter-bin density of 2.33 cM. A subset of DArT markers was also mapped physically using a set of wheat-H. chilense chromosome addition lines. It allowed the unambiguous assignment of linkage groups to chromosomes. Four segregation distortion regions (SDRs) were found on the chromosomes 2H(ch), 3H(ch) and 5H(ch) in agreement with previous findings in barley. The new map improves the genome coverage of previous H. chilense maps. H. chilense-derived DArT markers will enable further genetic studies in ongoing projects on hybrid wheat, seed carotenoid content improvement or tritordeum breeding program. Besides, the genetic map reported here will be very useful as the basis to develop comparative genomics studies with barley and model species.

Citing Articles

QTL Mapping and Phenotypic Variation for Seedling Vigour Traits in Barley ( L.).

Capo-Chichi L, Eldridge S, Elakhdar A, Kubo T, Brueggeman R, Anyia A Plants (Basel). 2021; 10(6).

PMID: 34200109 PMC: 8227620. DOI: 10.3390/plants10061149.

Tritordeum: Creating a New Crop Species-The Successful Use of Plant Genetic Resources.

Avila C, Rodriguez-Suarez C, Atienza S Plants (Basel). 2021; 10(5).

PMID: 34065483 PMC: 8161160. DOI: 10.3390/plants10051029.

Back into the wild-Apply untapped genetic diversity of wild relatives for crop improvement.

Zhang H, Mittal N, Leamy L, Barazani O, Song B Evol Appl. 2016; 10(1):5-24.

PMID: 28035232 PMC: 5192947. DOI: 10.1111/eva.12434.

Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background.

Rey M, Calderon M, Rodrigo M, Zacarias L, Alos E, Prieto P PLoS One. 2015; 10(8):e0134598.

PMID: 26241856 PMC: 4524710. DOI: 10.1371/journal.pone.0134598.

Fertility of CMS wheat is restored by two Rf loci located on a recombined acrocentric chromosome.

Castillo A, Atienza S, Martin A J Exp Bot. 2014; 65(22):6667-77.

PMID: 25271260 PMC: 4246193. DOI: 10.1093/jxb/eru388.

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