Fine-mapping of QRfg2, a QTL for Resistance to Gibberella Stalk Rot in Maize

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2011 Nov 4

PMID 22048640

Citations 22

Authors

Dongfeng Zhang

Yongjie Liu

Yanling Guo

Qin Yang

Jianrong Ye

Shaojiang Chen

Mingliang Xu

Affiliations

Soon will be listed here.

Abstract

Stalk rot is one of the most devastating diseases in maize worldwide. In our previous study, two QTLs, a major qRfg1 and a minor qRfg2, were identified in the resistant inbred line '1145' to confer resistance to Gibberella stalk rot. In the present study, we report on fine-mapping of the minor qRfg2 that is located on chromosome 1 and account for ~8.9% of the total phenotypic variation. A total of 22 markers were developed in the qRfg2 region to resolve recombinants. The progeny-test mapping strategy was developed to accurately determine the phenotypes of all recombinants for fine-mapping of the qRfg2 locus. This fine-mapping process was performed from BC(4)F(1) to BC(8)F(1) generations to narrow down the qRfg2 locus into ~300 kb, flanked by the markers SSRZ319 and CAPSZ459. A predicted gene in the mapped region, coding for an auxin-regulated protein, is believed to be a candidate for qRfg2. The qRfg2 locus could steadily increase the resistance percentage by ~12% across different backcross generations, suggesting its usefulness in enhancing maize resistance against Gibberella stalk rot.

Citing Articles

Exploiting genomic tools for genetic dissection and improving the resistance to Fusarium stalk rot in tropical maize.

Song J, Liu Y, Guo R, Pacheco A, Munoz-Zavala C, Song W Theor Appl Genet. 2024; 137(5):109.

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Genetic dissection of maize disease resistance and its applications in molecular breeding.

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Identification and validation of a key genomic region on chromosome 6 for resistance to Fusarium stalk rot in tropical maize.

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Multi-Omics Analysis Reveals a Regulatory Network of ZmCCT During Maize Resistance to Gibberella Stalk Rot at the Early Stage.

Tang B, Zhang Z, Zhao X, Xu Y, Wang L, Chen X Front Plant Sci. 2022; 13:917493.

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Identification and Validation of Genomic Regions Associated With Charcoal Rot Resistance in Tropical Maize by Genome-Wide Association and Linkage Mapping.

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