Corticotropin-releasing Factor Secretion from Dendritic Cells Stimulated by Commensal Bacteria

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2011 Nov 3

PMID 22046091

Citations 8

Authors

Mariko Hojo

Toshifumi Ohkusa

Harumi Tomeoku

Shigeo Koido

Daisuke Asaoka

Akihito Nagahara

Sumio Watanabe

Affiliations

Soon will be listed here.

Abstract

Aim: To study the production and secretion of corticotropin-releasing factor (CRF) by dendritic cells and the influence of commensal bacteria.

Methods: JAWSII cells (ATCC CRL-11904), a mouse dendritic cell line, were seeded into 24-well culture plates and grown for 3 d. Commensal bacterial strains of Clostridium clostrodiiforme (JCM1291), Bacteroides vulgatus (B. vulgatus) (JCM5856), Escherichia coli (JCM1649), or Fusobacterium varium (F. varium) (ATCC8501) were added to the cells except for the control well, and incubated for 2 h. After incubation, we performed enzyme-linked immunosorbent assay for the cultured medium and reverse transcription polymerase chain reaction for the dendritic cells, and compared these values with controls.

Results: The level of CRF secretion by control dendritic cells was 40.4 ± 6.2 pg/mL. The CRF levels for cells incubated with F. varium and B. vulgatus were significantly higher than that of the control (P < 0.0001). CRF mRNA was present in the control sample without bacteria, and CRF mRNA levels in all samples treated with bacteria were above that of the control sample. F. varium caused the greatest increase in CRF mRNA expression.

Conclusion: Our results suggest that dendritic cells produce CRF, a process augmented by commensal bacteria.

Citing Articles

Pathophysiological Commonality Between Irritable Bowel Syndrome and Metabolic Syndrome: Role of Corticotropin-releasing Factor-Toll-like Receptor 4-Proinflammatory Cytokine Signaling.

Nozu T, Okumura T J Neurogastroenterol Motil. 2022; 28(2):173-184.

PMID: 35189599 PMC: 8978123. DOI: 10.5056/jnm21002.

Chronic Stress, Inflammation, and Colon Cancer: A CRH System-Driven Molecular Crosstalk.

Baritaki S, de Bree E, Chatzaki E, Pothoulakis C J Clin Med. 2019; 8(10).

PMID: 31614860 PMC: 6833069. DOI: 10.3390/jcm8101669.

Current insights into the innate immune system dysfunction in irritable bowel syndrome.

Lazaridis N, Germanidis G Ann Gastroenterol. 2018; 31(2):171-187.

PMID: 29507464 PMC: 5825947. DOI: 10.20524/aog.2018.0229.

Involvement of Corticotropin-Releasing Factor and Receptors in Immune Cells in Irritable Bowel Syndrome.

Chatoo M, Li Y, Ma Z, Coote J, Du J, Chen X Front Endocrinol (Lausanne). 2018; 9:21.

PMID: 29483895 PMC: 5816029. DOI: 10.3389/fendo.2018.00021.

Corticotropin-releasing factor augments LPS-induced immune/inflammatory responses in JAWSII cells.

Hu Y, Li M, Lu B, Wang X, Chen C, Zhang M Immunol Res. 2016; 64(2):540-7.

PMID: 26754762 DOI: 10.1007/s12026-015-8740-3.

References

Lembo T, Plourde V, Shui Z, Fullerton S, Mertz H, Tache Y . Effects of the corticotropin-releasing factor (CRF) on rectal afferent nerves in humans. Neurogastroenterol Motil. 1996; 8(1):9-18. DOI: 10.1111/j.1365-2982.1996.tb00237.x. View

Whitehead W, Holtkotter B, Enck P, Hoelzl R, Holmes K, Anthony J . Tolerance for rectosigmoid distention in irritable bowel syndrome. Gastroenterology. 1990; 98(5 Pt 1):1187-92. DOI: 10.1016/0016-5085(90)90332-u. View

Sagami Y, Shimada Y, Tayama J, Nomura T, Satake M, Endo Y . Effect of a corticotropin releasing hormone receptor antagonist on colonic sensory and motor function in patients with irritable bowel syndrome. Gut. 2004; 53(7):958-64. PMC: 1774093. DOI: 10.1136/gut.2003.018911. View

Lee H, Kwon Y, Park C, Oh S, Lee J, Wu W . Corticotropin-releasing factor decreases IL-18 in the monocyte-derived dendritic cell. Exp Dermatol. 2008; 18(3):199-204. DOI: 10.1111/j.1600-0625.2008.00781.x. View

Bonaz B, Tache Y . Water-avoidance stress-induced c-fos expression in the rat brain and stimulation of fecal output: role of corticotropin-releasing factor. Brain Res. 1994; 641(1):21-8. DOI: 10.1016/0006-8993(94)91810-4. View

Schulte H, Chrousos G, Gold P, BOOTH J, Oldfield E, Cutler Jr G . Continuous administration of synthetic ovine corticotropin-releasing factor in man. Physiological and pathophysiological implications. J Clin Invest. 1985; 75(6):1781-5. PMC: 425532. DOI: 10.1172/JCI111890. View

Williams C, Peterson J, Villar R, Burks T . Corticotropin-releasing factor directly mediates colonic responses to stress. Am J Physiol. 1987; 253(4 Pt 1):G582-6. DOI: 10.1152/ajpgi.1987.253.4.G582. View

Kanazawa M, Endo Y, Whitehead W, Kano M, Hongo M, Fukudo S . Patients and nonconsulters with irritable bowel syndrome reporting a parental history of bowel problems have more impaired psychological distress. Dig Dis Sci. 2004; 49(6):1046-53. DOI: 10.1023/b:ddas.0000034570.52305.10. View

Kawahito Y, Sano H, Kawata M, Yuri K, Mukai S, Yamamura Y . Local secretion of corticotropin-releasing hormone by enterochromaffin cells in human colon. Gastroenterology. 1994; 106(4):859-65. DOI: 10.1016/0016-5085(94)90743-9. View

10.

Pimentel M, Chow E, Lin H . Normalization of lactulose breath testing correlates with symptom improvement in irritable bowel syndrome. a double-blind, randomized, placebo-controlled study. Am J Gastroenterol. 2003; 98(2):412-9. DOI: 10.1111/j.1572-0241.2003.07234.x. View

11.

Ekman R, Servenius B, Castro M, Lowry P, Cederlund A, Bergman O . Biosynthesis of corticotropin-releasing hormone in human T-lymphocytes. J Neuroimmunol. 1993; 44(1):7-13. DOI: 10.1016/0165-5728(93)90262-w. View

12.

Ohkusa T, Okayasu I, Ogihara T, Morita K, Ogawa M, Sato N . Induction of experimental ulcerative colitis by Fusobacterium varium isolated from colonic mucosa of patients with ulcerative colitis. Gut. 2002; 52(1):79-83. PMC: 1773498. DOI: 10.1136/gut.52.1.79. View

13.

Cruickshank S, English N, Felsburg P, Carding S . Characterization of colonic dendritic cells in normal and colitic mice. World J Gastroenterol. 2006; 11(40):6338-47. PMC: 4320338. DOI: 10.3748/wjg.v11.i40.6338. View

14.

Sadlack B, Merz H, Schorle H, Schimpl A, Feller A, Horak I . Ulcerative colitis-like disease in mice with a disrupted interleukin-2 gene. Cell. 1993; 75(2):253-61. DOI: 10.1016/0092-8674(93)80067-o. View

15.

Tornblom H, Lindberg G, Nyberg B, Veress B . Full-thickness biopsy of the jejunum reveals inflammation and enteric neuropathy in irritable bowel syndrome. Gastroenterology. 2002; 123(6):1972-9. DOI: 10.1053/gast.2002.37059. View

16.

Sellon R, Tonkonogy S, Schultz M, Dieleman L, Grenther W, Balish E . Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice. Infect Immun. 1998; 66(11):5224-31. PMC: 108652. DOI: 10.1128/IAI.66.11.5224-5231.1998. View

17.

Barquist E, Zinner M, Rivier J, Tache Y . Abdominal surgery-induced delayed gastric emptying in rats: role of CRF and sensory neurons. Am J Physiol. 1992; 262(4 Pt 1):G616-20. DOI: 10.1152/ajpgi.1992.262.4.G616. View

18.

Kishi D, Takahashi I, Kai Y, Tamagawa H, Iijima H, Obunai S . Alteration of V beta usage and cytokine production of CD4+ TCR beta beta homodimer T cells by elimination of Bacteroides vulgatus prevents colitis in TCR alpha-chain-deficient mice. J Immunol. 2000; 165(10):5891-9. DOI: 10.4049/jimmunol.165.10.5891. View

19.

Petrusz P, Merchenthaler I, Maderdrut J, Vigh S, Schally A . Corticotropin-releasing factor (CRF)-like immunoreactivity in the vertebrate endocrine pancreas. Proc Natl Acad Sci U S A. 1983; 80(6):1721-5. PMC: 393675. DOI: 10.1073/pnas.80.6.1721. View

20.

Crofford L, Sano H, Karalis K, Friedman T, Epps H, Remmers E . Corticotropin-releasing hormone in synovial fluids and tissues of patients with rheumatoid arthritis and osteoarthritis. J Immunol. 1993; 151(3):1587-96. View