Monaural Conductive Hearing Loss Alters the Expression of the GluA3 AMPA and Glycine Receptor α1 Subunits in Bushy and Fusiform Cells of the Cochlear Nucleus

Overview

Journal Neuroscience

Specialty Neurology

Date 2011 Nov 3

PMID 22044924

Citations 19

Authors

H Wang

G Yin

K Rogers

C Miralles

A L De Blas

M E Rubio

Affiliations

Soon will be listed here.

Abstract

The impact of conductive hearing loss (CHL), the second most common form of hearing loss, on neuronal plasticity in the central auditory pathway is unknown. After short-term (1 day) monaural earplugging, the GluA3 subunits of the AMPA receptor (AMPAR) are upregulated at auditory nerve synapses on the projection neurons of the cochlear nucleus; glycine receptor α1 (GlyRα1) subunits are downregulated at inhibitory synapses in the same neuronal population. These data suggest that CHL affects receptor trafficking at synapses. We examined the impact of 7 days of CHL on the general expression of excitatory and inhibitory receptors by quantitative biochemistry and immunohistochemistry, using specific antibodies to detect AMPAR subunits (GluA1, GluA2, GluA2/3, and GluA4), GlyRα1, and the GABA(A) receptor subunits β2/3. Following monaural earplugging and an elevation of the hearing threshold by approximately 35 dB, the immunolabeling of the antibody for the GluA2/3 subunits but not the GluA2 subunit increased on bushy cells (BCs) and fusiform cells (FCs) of the ipsilateral ventral and dorsal cochlear nuclei. These same cell types showed a downregulation of the GlyRα1 subunit. Similar results were observed in the contralateral nuclei. The expression levels of GABA(A) β2/3 were unchanged. These findings suggest that, following longer periods of monaural conductive hearing loss, the synthesis and subsequent composition of specific glutamate and glycine receptors in projection neurons and their synapses are altered; these changes may contribute to abnormal auditory processing.

Citing Articles

Glycine is a transmitter in the human and chimpanzee cochlear nuclei.

Baizer J, Sherwood C, Hof P, Baker J, Witelson S Front Neuroanat. 2024; 18:1331230.

PMID: 38425805 PMC: 10902441. DOI: 10.3389/fnana.2024.1331230.

GluA3 subunits are required for appropriate assembly of AMPAR GluA2 and GluA4 subunits on cochlear afferent synapses and for presynaptic ribbon modiolar-pillar morphology.

Rutherford M, Bhattacharyya A, Xiao M, Cai H, Pal I, Rubio M Elife. 2023; 12.

PMID: 36648432 PMC: 9891727. DOI: 10.7554/eLife.80950.

Increasing endogenous activity of NMDARs on GABAergic neurons increases inhibition, alters sensory processing and prevents noise-induced tinnitus.

Deng D, Masri S, Yao L, Ma X, Cao X, Yang S Sci Rep. 2020; 10(1):11969.

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Role of GluA3 AMPA Receptor Subunits in the Presynaptic and Postsynaptic Maturation of Synaptic Transmission and Plasticity of Endbulb-Bushy Cell Synapses in the Cochlear Nucleus.

Antunes F, Rubio M, Kandler K J Neurosci. 2020; 40(12):2471-2484.

PMID: 32051325 PMC: 7083531. DOI: 10.1523/JNEUROSCI.2573-19.2020.

Chronic Conductive Hearing Loss Is Associated With Speech Intelligibility Deficits in Patients With Normal Bone Conduction Thresholds.

Okada M, Welling D, Liberman M, Maison S Ear Hear. 2019; 41(3):500-507.

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