Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues

Overview

Journal J Histochem Cytochem

Publisher Sage Publications

Specialty Biochemistry

Date 2011 Nov 2

PMID 22043023

Citations 22

Authors

Monica Prasad

Qinglin Zhu

Yao Sun

Xiaofang Wang

Ashok Kulkarni

Adele Boskey

Jian Q Feng

Chunlin Qin

Affiliations

Soon will be listed here.

Abstract

Dentin sialophosphoprotein (DSPP) and its cleaved products, dentin phosphoprotein (DPP) and dentin sialoprotein (DSP), play important roles in biomineralization. Believed to be tooth specific, the authors' group revealed its expression in bone, and more recently, they and other groups also showed its expression in a few types of soft tissues. In this study, the authors systematically examined the expression of DSPP in a variety of non-mineralized tissues using reverse-transcription polymerase chain reaction (RT-PCR), real-time PCR, Western immunoblotting, and immunohistochemistry analyses in wild-type mice as well as β-galactosidase assays in the Dspp lacZ knock-in mice. These approaches showed the presence of DSPP in the salivary glands, cartilage, liver, kidney, and brain and its absence in the heart and spleen. Real-time PCR showed that the expression levels of DSPP mRNA in salivary glands, cartilage, liver, and kidney were higher than in the bone. Interestingly, DSPP was observed in the pericytes of blood vessels in the dental pulp, which are believed to be able to differentiate into odontoblasts. On the basis of these observations, the authors conclude that DSPP and/or its cleaved products may fulfill important functions in certain non-mineralized tissues in addition to its role in biomineralization.

Citing Articles

Intracranial calcification in Fam20c-deficient mice recapitulates human Raine syndrome.

Zhang H, Lu Y, Kramer P, Benson M, Cheng Y, Qin C Neurosci Lett. 2023; 802:137176.

PMID: 36914045 PMC: 11795667. DOI: 10.1016/j.neulet.2023.137176.

The role of Dentin Sialophosphoprotein (DSPP) in craniofacial development.

Figueredo C, Abdelhay N, Ganatra S, Gibson M J Oral Biol Craniofac Res. 2022; 12(5):673-678.

PMID: 36062256 PMC: 9434041. DOI: 10.1016/j.jobcr.2022.08.010.

The effect of BMP4, FGF8 and WNT3a on mouse iPS cells differentiating to odontoblast-like cells.

Takada K, Odashima A, Onodera S, Saito A, Aida N, Furusawa M Med Mol Morphol. 2022; 55(3):199-209.

PMID: 35578118 DOI: 10.1007/s00795-022-00318-9.

Effects of Gene Mutations on Periodontal Tissues.

Jing Z, Chen Z, Jiang Y Glob Med Genet. 2021; 8(3):90-94.

PMID: 34430959 PMC: 8378919. DOI: 10.1055/s-0041-1726416.

Identification of Novel Candidate Genes and Variants for Hearing Loss and Temporal Bone Anomalies.

Santos-Cortez R, Yarza T, Bootpetch T, Tantoco M, Mohlke K, Cruz T Genes (Basel). 2021; 12(4).

PMID: 33924653 PMC: 8069784. DOI: 10.3390/genes12040566.

References

Qin C, Brunn J, Cadena E, Ridall A, Tsujigiwa H, Nagatsuka H . The expression of dentin sialophosphoprotein gene in bone. J Dent Res. 2002; 81(6):392-4. DOI: 10.1177/154405910208100607. View

Ogbureke K, Fisher L . SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J Histochem Cytochem. 2007; 55(4):403-9. DOI: 10.1369/jhc.6A7075.2007. View

Fisher L, Torchia D, Fohr B, Young M, Fedarko N . Flexible structures of SIBLING proteins, bone sialoprotein, and osteopontin. Biochem Biophys Res Commun. 2001; 280(2):460-5. DOI: 10.1006/bbrc.2000.4146. View

Ogbureke K, Nikitakis N, Warburton G, Ord R, Sauk J, Waller J . Up-regulation of SIBLING proteins and correlation with cognate MMP expression in oral cancer. Oral Oncol. 2007; 43(9):920-32. DOI: 10.1016/j.oraloncology.2006.11.011. View

Terasawa M, Shimokawa R, Terashima T, Ohya K, Takagi Y, Shimokawa H . Expression of dentin matrix protein 1 (DMP1) in nonmineralized tissues. J Bone Miner Metab. 2004; 22(5):430-8. DOI: 10.1007/s00774-004-0504-4. View

Diaz-Flores L, Gutierrez R, Lopez-Alonso A, Gonzalez R, Varela H . Pericytes as a supplementary source of osteoblasts in periosteal osteogenesis. Clin Orthop Relat Res. 1992; (275):280-6. View

George A, Bannon L, Sabsay B, Dillon J, Malone J, Veis A . The carboxyl-terminal domain of phosphophoryn contains unique extended triplet amino acid repeat sequences forming ordered carboxyl-phosphate interaction ridges that may be essential in the biomineralization process. J Biol Chem. 1996; 271(51):32869-73. DOI: 10.1074/jbc.271.51.32869. View

Feng J, Zhang J, Tan X, Lu Y, Guo D, Harris S . Identification of cis-DNA regions controlling Bmp4 expression during tooth morphogenesis in vivo. J Dent Res. 2002; 81(1):6-10. DOI: 10.1177/002203450208100103. View

Yamamura T . Differentiation of pulpal cells and inductive influences of various matrices with reference to pulpal wound healing. J Dent Res. 1985; 64 Spec No:530-40. DOI: 10.1177/002203458506400406. View

10.

Zhu Q, Sun Y, Prasad M, Wang X, Yamoah A, Li Y . Glycosaminoglycan chain of dentin sialoprotein proteoglycan. J Dent Res. 2010; 89(8):808-12. PMC: 2909365. DOI: 10.1177/0022034510366902. View

11.

Xiao S, Yu C, Chou X, Yuan W, Wang Y, Bu L . Dentinogenesis imperfecta 1 with or without progressive hearing loss is associated with distinct mutations in DSPP. Nat Genet. 2001; 27(2):201-4. DOI: 10.1038/84848. View

12.

Qin C, Brunn J, Baba O, Wygant J, McIntyre B, Butler W . Dentin sialoprotein isoforms: detection and characterization of a high molecular weight dentin sialoprotein. Eur J Oral Sci. 2003; 111(3):235-42. DOI: 10.1034/j.1600-0722.2003.00043.x. View

13.

Qin C, Brunn J, Cadena E, Ridall A, Butler W . Dentin sialoprotein in bone and dentin sialophosphoprotein gene expressed by osteoblasts. Connect Tissue Res. 2003; 44 Suppl 1:179-83. View

14.

Ogbureke K, Fisher L . Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004; 83(9):664-70. DOI: 10.1177/154405910408300902. View

15.

Zhang J, Tan X, Contag C, Lu Y, Guo D, Harris S . Dissection of promoter control modules that direct Bmp4 expression in the epithelium-derived components of hair follicles. Biochem Biophys Res Commun. 2002; 293(5):1412-9. DOI: 10.1016/S0006-291X(02)00416-3. View

16.

Butler W, Bhown M, Brunn J, DSouza R, Farach-Carson M, Happonen R . Isolation, characterization and immunolocalization of a 53-kDal dentin sialoprotein (DSP). Matrix. 1992; 12(5):343-51. DOI: 10.1016/s0934-8832(11)80030-2. View

17.

Sreenath T, Thyagarajan T, Hall B, Longenecker G, DSouza R, Hong S . Dentin sialophosphoprotein knockout mouse teeth display widened predentin zone and develop defective dentin mineralization similar to human dentinogenesis imperfecta type III. J Biol Chem. 2003; 278(27):24874-80. DOI: 10.1074/jbc.M303908200. View

18.

Shi S, Gronthos S . Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res. 2003; 18(4):696-704. DOI: 10.1359/jbmr.2003.18.4.696. View

19.

Brighton C, Lorich D, Kupcha R, Reilly T, Jones A, Woodbury 2nd R . The pericyte as a possible osteoblast progenitor cell. Clin Orthop Relat Res. 1992; (275):287-99. View

20.

Baba O, Qin C, Brunn J, Jones J, Wygant J, McIntyre B . Detection of dentin sialoprotein in rat periodontium. Eur J Oral Sci. 2004; 112(2):163-70. DOI: 10.1111/j.0909-8836.2004.00110.x. View