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Fast Calcium and Voltage-sensitive Dye Imaging in Enteric Neurones Reveal Calcium Peaks Associated with Single Action Potential Discharge

Overview
Journal J Physiol
Specialty Physiology
Date 2011 Nov 2
PMID 22041184
Citations 22
Authors
K Michel
M Michaelis
G Mazzuoli
K Mueller
P Vanden Berghe
M Schemann
Affiliations
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Abstract

Slow changes in [Ca(2+)](i) reflect increased neuronal activity. Our study demonstrates that single-trial fast [Ca(2+)](i) imaging (≥200 Hz sampling rate) revealed peaks each of which are associated with single spike discharge recorded by consecutive voltage-sensitive dye (VSD) imaging in enteric neurones and nerve fibres. Fast [Ca(2+)](i) imaging also revealed subthreshold fast excitatory postsynaptic potentials. Nicotine-evoked [Ca(2+)](i) peaks were reduced by -conotoxin and blocked by ruthenium red or tetrodotoxin. Fast [Ca(2+)](i) imaging can be used to directly record single action potentials in enteric neurones. [Ca(2+)](i) peaks required opening of voltage-gated sodium and calcium channels as well as Ca(2+) release from intracellular stores.

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