A Novel Mechanism of Increased Infections in Contact Lens Wearers

Overview

Journal Invest Ophthalmol Vis Sci

Specialty Ophthalmology

Date 2011 Nov 1

PMID 22039229

Citations 7

Authors

Gabriel M Gordon

Navid Moradshahi

Shinwu Jeong

Christianne Lane

M Elizabeth Fini

Affiliations

Soon will be listed here.

Abstract

Purpose: It is well documented that contact lens wearers have much higher incidences of corneal infections compared with those of non-contact lens wearers, although the exact cause(s) of this increased susceptibility has not been identified. A distinct subset of mucins (MUCs) is present on the ocular surface, acting to protect the integrity of the corneal epithelium. This study was performed to determine whether multipurpose contact lens solutions (MPCLSs) can cause increased infections in the cornea by destroying the protective cell-bound mucin layer.

Methods: An immortalized human corneal limbal epithelial cell line was treated in the presence of four commonly used MPCLSs or PBS and the expression and release of MUC-16 was assessed. Cells were also cultured with Pseudomonas aeruginosa after MPCLS treatment and internalization of bacteria was assessed by quantitative genomic PCR. Loss of MUC-16 was then correlated with infection rates.

Results: Each of the four commonly used MPCLSs examined in this study differentially affected mucin release. The relative effect was correlated with an increase in infection of corneal epithelial cells by P. aeruginosa.

Conclusions: The results of this study are consistent with the hypothesis that MPCLSs cause increased infections in the cornea by destroying the protective cell-bound mucin layer.

Citing Articles

Membrane-associated mucins of the human ocular surface in health and disease.

Martinez-Carrasco R, Argueso P, Fini M Ocul Surf. 2021; 21:313-330.

PMID: 33775913 PMC: 8328898. DOI: 10.1016/j.jtos.2021.03.003.

Membrane-associated mucins of the ocular surface: New genes, new protein functions and new biological roles in human and mouse.

Fini M, Jeong S, Gong H, Martinez-Carrasco R, Laver N, Hijikata M Prog Retin Eye Res. 2019; 75:100777.

PMID: 31493487 PMC: 10276350. DOI: 10.1016/j.preteyeres.2019.100777.

Dynasore protects the ocular surface against damaging oxidative stress.

Webster A, Chintala S, Kim J, Ngan M, Itakura T, Panjwani N PLoS One. 2018; 13(10):e0204288.

PMID: 30303976 PMC: 6179211. DOI: 10.1371/journal.pone.0204288.

Evaluation of the safety and efficacy of therapeutic bandage contact lenses on post-cataract surgery patients.

Shi D, Song H, Ding T, Qiu W, Wang W Int J Ophthalmol. 2018; 11(2):230-234.

PMID: 29487811 PMC: 5824076. DOI: 10.18240/ijo.2018.02.08.

A proof-of-concept model for the identification of the key events in the infection process with specific reference to Pseudomonas aeruginosa in corneal infections.

Soumpasis I, Knapp L, Pitt T Infect Ecol Epidemiol. 2015; 5:28750.

PMID: 26546946 PMC: 4636861. DOI: 10.3402/iee.v5.28750.

References

Singh P, Hollingsworth M . Cell surface-associated mucins in signal transduction. Trends Cell Biol. 2006; 16(9):467-76. DOI: 10.1016/j.tcb.2006.07.006. View

Blalock T, Spurr-Michaud S, Tisdale A, Heimer S, Gilmore M, Ramesh V . Functions of MUC16 in corneal epithelial cells. Invest Ophthalmol Vis Sci. 2007; 48(10):4509-18. DOI: 10.1167/iovs.07-0430. View

Gipson I, Spurr-Michaud S, Argueso P, Tisdale A, Ng T, Russo C . Mucin gene expression in immortalized human corneal-limbal and conjunctival epithelial cell lines. Invest Ophthalmol Vis Sci. 2003; 44(6):2496-506. DOI: 10.1167/iovs.02-0851. View

Bennett H, Hay J, Kirkness C, Seal D, Devonshire P . Antimicrobial management of presumed microbial keratitis: guidelines for treatment of central and peripheral ulcers. Br J Ophthalmol. 1998; 82(2):137-45. PMC: 1722498. DOI: 10.1136/bjo.82.2.137. View

McClellan K, BERNARD P, Billson F . Microbial investigations in keratitis at the Sydney Eye Hospital. Aust N Z J Ophthalmol. 1989; 17(4):413-6. DOI: 10.1111/j.1442-9071.1989.tb00564.x. View

Keay L, Edwards K, Naduvilath T, Taylor H, Snibson G, Forde K . Microbial keratitis predisposing factors and morbidity. Ophthalmology. 2005; 113(1):109-16. DOI: 10.1016/j.ophtha.2005.08.013. View

Lipener C . A randomized clinical comparison of OPTI-FREE EXPRESS and ReNu MultiPLUS multipurpose lens care solutions. Adv Ther. 2009; 26(4):435-46. DOI: 10.1007/s12325-009-0023-8. View

Cruz O, Sabir S, Capo H, Alfonso E . Microbial keratitis in childhood. Ophthalmology. 1993; 100(2):192-6. DOI: 10.1016/s0161-6420(93)31671-4. View

Kodjikian L, Casoli-Bergeron E, Malet F, Janin-Manificat H, Freney J, Burillon C . Bacterial adhesion to conventional hydrogel and new silicone-hydrogel contact lens materials. Graefes Arch Clin Exp Ophthalmol. 2007; 246(2):267-73. DOI: 10.1007/s00417-007-0703-5. View

10.

Imayasu M, Hori Y, Cavanagh H . Effects of multipurpose contact lens care solutions and their ingredients on membrane-associated mucins of human corneal epithelial cells. Eye Contact Lens. 2010; 36(6):361-6. DOI: 10.1097/ICL.0b013e3181faa43e. View

11.

Goh P, Shamala R, Chandamalar S, Tai X . Contact lens--related corneal ulcer: a two-year review. Med J Malaysia. 2011; 65 Suppl A:120-3. View

12.

Jurkunas U, Behlau I, Colby K . Fungal keratitis: changing pathogens and risk factors. Cornea. 2009; 28(6):638-43. DOI: 10.1097/ICO.0b013e318191695b. View

13.

Lillehoj E, Hyun S, Kim B, Zhang X, Lee D, Rowland S . Muc1 mucins on the cell surface are adhesion sites for Pseudomonas aeruginosa. Am J Physiol Lung Cell Mol Physiol. 2001; 280(1):L181-7. DOI: 10.1152/ajplung.2001.280.1.L181. View

14.

Chang D, Grant G, ODonnell K, Wannemuehler K, Noble-Wang J, Rao C . Multistate outbreak of Fusarium keratitis associated with use of a contact lens solution. JAMA. 2006; 296(8):953-63. DOI: 10.1001/jama.296.8.953. View

15.

Epstein A . In the aftermath of the Fusarium keratitis outbreak: What have we learned?. Clin Ophthalmol. 2009; 1(4):355-66. PMC: 2704532. View

16.

Salz J, Schlanger J . Complications of aphakic extended wear lenses encountered during a seven-year period in 100 eyes. CLAO J. 1983; 9(3):241-4. View

17.

Levy B, Heiler D, Norton S . Report on testing from an investigation of fusarium keratitis in contact lens wearers. Eye Contact Lens. 2006; 32(6):256-61. DOI: 10.1097/01.icl.0000245556.46738.14. View

18.

Carvalho F, Foronda A, Mannis M, Hofling-Lima A, Belfort Jr R, de Freitas D . Twenty years of acanthamoeba keratitis. Cornea. 2009; 28(5):516-9. DOI: 10.1097/ICO.0b013e318193e0fe. View

19.

McAuley J, Linden S, Png C, King R, Pennington H, Gendler S . MUC1 cell surface mucin is a critical element of the mucosal barrier to infection. J Clin Invest. 2007; 117(8):2313-24. PMC: 1913485. DOI: 10.1172/JCI26705. View

20.

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View