A Diagnostic Test for Scabies: IgE Specificity for a Recombinant Allergen of Sarcoptes Scabiei
Overview
Microbiology
Pathology
Authors
Affiliations
Scabies infestations are difficult to diagnose clinically and current serologic tests have less than 50% accuracy. To develop more reliable diagnosis of scabies, specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma samples from scabies-infested and control subject groups using dissociation-enhanced lanthanide fluorescent immunoassays (DELFIA). Levels of rSar s 14.3-specific IgE were quantified, and cross-reactivity with its house dust mite homologue, Der p 14, was assessed. The rSar s 14.3 DELFIA showed excellent diagnostic capability, with 100% sensitivity and 93.75% specificity for distinguishing subjects with current scabies infestation from control, uninfested subjects. Recombinant Der p 14 preparation was ineffective at inhibiting IgE binding to rSar s 14.3. This study shows that quantification of levels of IgE antibody to rSar s 14.3 is a highly sensitive method for diagnosis of scabies infestation in clinical practice.
Fernando D, Mounsey K, Bernigaud C, Surve N, Estrada Chavez G, Hay R Nat Rev Dis Primers. 2024; 10(1):74.
PMID: 39362885 DOI: 10.1038/s41572-024-00552-8.
Scabies: Immunopathogenesis and pathological changes.
Sharaf M Parasitol Res. 2024; 123(3):149.
PMID: 38433167 PMC: 10909795. DOI: 10.1007/s00436-024-08173-6.
Laboratory-based diagnosis of scabies: a review of the current status.
Siddig E, Hay R Trans R Soc Trop Med Hyg. 2021; 116(1):4-9.
PMID: 33763705 PMC: 8776561. DOI: 10.1093/trstmh/trab049.
Shen N, Chen Y, Wei W, Xiong L, Tao Y, Xiao J Parasit Vectors. 2021; 14(1):148.
PMID: 33750446 PMC: 7941879. DOI: 10.1186/s13071-021-04654-0.
Prevalence of scabies in long-term care hospitals in South Korea.
Kim D, Yun S, Park Y, Kang S, Yu H PLoS Negl Trop Dis. 2020; 14(8):e0008554.
PMID: 32810163 PMC: 7433867. DOI: 10.1371/journal.pntd.0008554.