Prevalence Study of Bovine Viral Diarrhea Virus by Evaluation of Antigen Capture ELISA and RT-PCR Assay in Bovine, Ovine, Caprine, Buffalo and Camel Aborted Fetuses in Iran

Overview

Journal AMB Express

Date 2011 Oct 25

PMID 22018096

Citations 14

Authors

Farhad Safarpoor Dehkordi

Affiliations

Soon will be listed here.

Abstract

Bovine viral diarrhea virus is a pestivirus in the family Flaviviridae that cause abortions and stillbirths in livestock and its traditional diagnosis is based on cell culture and virus neutralization test. In this study, for more sensitive, specific detection and determined the prevalence of virus in aborted Bovine, Ovine, Caprine, Buffalo and Camel fetuses the antigen capture ELISA and RT-PCR were recommended. From the total of 2173 aborted fetuses, 347 (15.96%) and 402 (18.49%) were positive for presence of Bovine viral diarrhea virus by antigen capture ELISA and RT-PCR respectively. Statistical analysis of data showed significant differences between ELISA and RT-PCR for detection of virus in aborted fetuses.These results indicate a high presence of this pathogen in Iran and that RT- PCR is considerably faster and more accurate than ELISA for identification of Bovine viral diarrhea virus.To our knowledge the Camels and Bovine are the most resistant and sensitive to Bovine viral diarrhea's abortions respectively and the prevalence of virus in Caprine is more than Ovine aborted fetuses. This study is the first prevalence report of Bovine viral diarrhea virus in aborted Bovine, Ovine, Caprine, Buffalo and Camel fetuses by evaluation of ELISA and RT-PCR in Iran.

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References

Vanderheijden N, De Moerlooze L, Vandenbergh D, Chappuis G, Renard A, Lecomte C . Expression of the bovine viral diarrhoea virus Osloss p80 protein: its use as ELISA antigen for cattle serum antibody detection. J Gen Virol. 1993; 74 ( Pt 7):1427-31. DOI: 10.1099/0022-1317-74-7-1427. View

Vilcek S, Herring A, Herring J, Nettleton P, Lowings J, Paton D . Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol. 1994; 136(3-4):309-23. DOI: 10.1007/BF01321060. View

Donis R . Molecular biology of bovine viral diarrhea virus and its interactions with the host. Vet Clin North Am Food Anim Pract. 1995; 11(3):393-423. PMC: 7135131. DOI: 10.1016/s0749-0720(15)30459-x. View

Niza-Ribeiro J, Pereira A, Souza J, Madeira H, Barbosa A, Afonso C . Estimated BVDV-prevalence, -contact and -vaccine use in dairy herds in Northern Portugal. Prev Vet Med. 2005; 72(1-2):81-5. DOI: 10.1016/j.prevetmed.2005.06.005. View

Nettleton P . Pestivirus infections in ruminants other than cattle. Rev Sci Tech. 1990; 9(1):131-50. DOI: 10.20506/rst.9.1.485. View

Loken T . Ruminant pestivirus infections in animals other than cattle and sheep. Vet Clin North Am Food Anim Pract. 1995; 11(3):597-614. DOI: 10.1016/s0749-0720(15)30469-2. View

Buyukcangaz E, Sen A, Carli K, Kahya S . Comparison of direct culture versus PCR for the detection of Brucella in aborted fetuses of cattle and sheep in Turkey. Vet Rec. 2011; 168(16):430. DOI: 10.1136/vr.c7003. View

Billinis C, Leontides L, Amiridis G, Spyrou V, Kostoulas P, Sofia M . Prevalence of BVDV infection in Greek dairy herds. Prev Vet Med. 2005; 72(1-2):75-9. DOI: 10.1016/j.prevetmed.2005.05.011. View

Baker J . Bovine viral diarrhea virus: a review. J Am Vet Med Assoc. 1987; 190(11):1449-58. View

10.

Kennedy J, Mortimer R, Powers B . Reverse transcription-polymerase chain reaction on pooled samples to detect bovine viral diarrhea virus by using fresh ear-notch-sample supernatants. J Vet Diagn Invest. 2006; 18(1):89-93. DOI: 10.1177/104063870601800113. View

11.

Sudharshana K, Suresh K, Rajasekhar M . Prevalence of bovine viral diarrhoea virus antibodies in India. Rev Sci Tech. 1999; 18(3):667-71. DOI: 10.20506/rst.18.3.1189. View

12.

Passler T, Ditchkoff S, Givens M, Brock K, DeYoung R, Walz P . Transmission of bovine viral diarrhea virus among white-tailed deer (Odocoileus virginianus). Vet Res. 2009; 41(2):20. PMC: 2797653. DOI: 10.1051/vetres/2009068. View

13.

Baker J . The clinical manifestations of bovine viral diarrhea infection. Vet Clin North Am Food Anim Pract. 1995; 11(3):425-45. DOI: 10.1016/s0749-0720(15)30460-6. View

14.

Entrican G, DAND A, Nettleton P . A double monoclonal antibody ELISA for detecting pestivirus antigen in the blood of viraemic cattle and sheep. Vet Microbiol. 1995; 43(1):65-74. DOI: 10.1016/0378-1135(94)00081-7. View

15.

Gilbert S, Burton K, Prins S, Deregt D . Typing of bovine viral diarrhea viruses directly from blood of persistently infected cattle by multiplex PCR. J Clin Microbiol. 1999; 37(6):2020-3. PMC: 85017. DOI: 10.1128/JCM.37.6.2020-2023.1999. View

16.

Passler T, Walz P, Ditchkoff S, Givens M, Maxwell H, Brock K . Experimental persistent infection with bovine viral diarrhea virus in white-tailed deer. Vet Microbiol. 2007; 122(3-4):350-6. DOI: 10.1016/j.vetmic.2007.01.028. View

17.

Lamm C, Broaddus C, Holyoak G . Distribution of bovine viral diarrhea virus antigen in aborted fetal and neonatal goats by immunohistochemistry. Vet Pathol. 2008; 46(1):54-8. DOI: 10.1354/vp.46-1-54. View

18.

Cornish T, van Olphen A, Cavender J, Edwards J, Jaeger P, Vieyra L . Comparison of ear notch immunohistochemistry, ear notch antigen-capture ELISA, and buffy coat virus isolation for detection of calves persistently infected with bovine viral diarrhea virus. J Vet Diagn Invest. 2005; 17(2):110-7. DOI: 10.1177/104063870501700203. View

19.

HAMEL A, Wasylyshen M, Nayar G . Rapid detection of bovine viral diarrhea virus by using RNA extracted directly from assorted specimens and a one-tube reverse transcription PCR assay. J Clin Microbiol. 1995; 33(2):287-91. PMC: 227934. DOI: 10.1128/jcm.33.2.287-291.1995. View

20.

Mackay I . Real-time PCR in the microbiology laboratory. Clin Microbiol Infect. 2004; 10(3):190-212. DOI: 10.1111/j.1198-743x.2004.00722.x. View