Synthesis, Separation, and Characterization of Amphiphilic Sulfated Oligosaccharides Enabled by Reversed-phase Ion Pairing LC and LC-MS Methods

Overview

Journal Carbohydr Res

Publisher Elsevier

Specialties Biochemistry
Endocrinology

Date 2011 Oct 22

PMID 22015170

Citations 4

Authors

Amanda M Fenner

Robert J Kerns

Affiliations

Soon will be listed here.

Abstract

Synthesis of amphiphilic oligosaccharides is problematic because traditional methods for separating and purifying oligosaccharides, including sulfated oligosaccharides, are generally not applicable to working with amphiphilic sugars. We report here RPIP-LC and LC-MS methods that enable the synthesis, separation, and characterization of amphiphilic N-arylacyl O-sulfonated aminoglycosides, which are being pursued as small-molecule glycosaminoglycan mimics. The methods described in this work for separating and characterizing these amphiphilic saccharides are further applied to a number of uses: monitoring the progression of sulfonation reactions with analytical RP-HPLC, characterizing sulfate content for individual molecules with ESI-MS, determining the degree of sulfation for products having mixed degrees of sulfation with HPLC and LC-MS, and purifying products with benchtop C18 column chromatography. We believe that the methods described here will be broadly applicable to enabling the synthesis, separation, and characterization of amphiphilic, sulfated, and phosphorylated oligosaccharides and other types of molecules substituted to varying degrees with both anionic and hydrophobic groups.

Citing Articles

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