Determination of Low Levels of Lead in Beer Using Solid-phase Extraction and Detection by Flame Atomic Absorption Spectrometry

Overview

Journal J Autom Methods Manag Chem

Date 2011 Oct 21

PMID 22013389

Authors

Vanessa N Alves

Simone S O Borges

Waldomiro B Neto

Nivia M M Coelho

Affiliations

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Abstract

In this study, a method for the determination of low concentrations of lead in beer samples using solid-phase extraction with a flow injection analysis system and detection by flame atomic absorption spectrometry (FAAS) was developed. Moringa oleifera seeds were used as a biosorbent material. Chemical and flow variables of the online preconcentration system, such as sample pH, preconcentration flow rate, eluent flow rate, eluent concentration, particle size, and sorbent mass, were studied. The optimum extraction conditions were obtained using a sample pH of 6.0, sample flow rate of 6.0 mL min(-1), 63.0 mg of sorbent mass, and 2.0 mol L(-1) HNO(3) at a flow rate of 2.0 mL min(-1) as the eluent. With the optimized conditions, the preconcentration factor, precision, detection limit, consumption index, and sample throughput were estimated as 93, 0.3% (10.0 μg L(-1), n = 7), 7.5 μg L(-1), 0.11 mL, and 23 samples per hour, respectively. The method developed was successfully applied to beer samples and recovery tests, with recovery ranging from 80% to 100%.

References

Tuzen M, Soylak M . Multi-element coprecipitation for separation and enrichment of heavy metal ions for their flame atomic absorption spectrometric determinations. J Hazard Mater. 2008; 162(2-3):724-9. DOI: 10.1016/j.jhazmat.2008.05.087. View

Melek E, Tuzen M, Soylak M . Flame atomic absorption spectrometric determination of cadmium(II) and lead(II) after their solid phase extraction as dibenzyldithiocarbamate chelates on Dowex Optipore V-493. Anal Chim Acta. 2007; 578(2):213-9. DOI: 10.1016/j.aca.2006.07.003. View

Gassenschmidt U, Jany K, Tauscher B, Niebergall H . Isolation and characterization of a flocculating protein from Moringa oleifera Lam. Biochim Biophys Acta. 1995; 1243(3):477-81. DOI: 10.1016/0304-4165(94)00176-x. View

Citak D, Tuzen M . A novel preconcentration procedure using cloud point extraction for determination of lead, cobalt and copper in water and food samples using flame atomic absorption spectrometry. Food Chem Toxicol. 2010; 48(5):1399-404. DOI: 10.1016/j.fct.2010.03.008. View

Tuzen M, Uluozlu O, Usta C, Soylak M . Biosorption of copper(II), lead(II), iron(III) and cobalt(II) on Bacillus sphaericus-loaded Diaion SP-850 resin. Anal Chim Acta. 2007; 581(2):241-6. DOI: 10.1016/j.aca.2006.08.040. View