Hydrogen Autotrophy of Nocardia Opaca Strains is Encoded by Linear Megaplasmids

Overview

Journal J Gen Microbiol

Specialty Microbiology

Date 1990 Jun 1

PMID 2200846

Citations 16

Authors

J Kalkus

M Reh

H G Schlegel

Affiliations

Soon will be listed here.

Abstract

Several linear megaplasmids were detected in the facultatively lithoautotrophic Gram-positive bacterium Nocardia opaca. The wild-type strain MR11 contains, in addition to the cccDNA plasmids pHG31-a and pHG31-b, the linear plasmids pHG201 (270 kb), pHG202 (400 kb) and pHG203 (420 kb). The wild-type strain MR22 contains, in addition to the cccDNA plasmid pHG33, the linear plasmids pHG204 (180 kb), pHG205 (280 kb) and pHG206 (510 kb). After preparation of DNA from cells embedded in agarose, the linear plasmids were demonstrated by pulsed-field electrophoresis. By means of DNA probes for genes of soluble hydrogenase and ribulose-bisphosphate carboxylase, the conjugative plasmids pHG201 and pHG205 were shown to be the carriers of the genetic information for these enzymes. A restriction map of pHG201 for the enzymes AsnI, SpeI, XbaI is presented.

Citing Articles

Production of biopolymer precursors beta-alanine and L-lactic acid from CO with metabolically versatile DSM 43205.

Salusjarvi L, Ojala L, Peddinti G, Lienemann M, Jouhten P, Pitkanen J Front Bioeng Biotechnol. 2022; 10:989481.

PMID: 36281430 PMC: 9587121. DOI: 10.3389/fbioe.2022.989481.

Transmission dynamics of a linear vanA-plasmid during a nosocomial multiclonal outbreak of vancomycin-resistant enterococci in a non-endemic area, Japan.

Fujiya Y, Harada T, Sugawara Y, Akeda Y, Yasuda M, Masumi A Sci Rep. 2021; 11(1):14780.

PMID: 34285270 PMC: 8292306. DOI: 10.1038/s41598-021-94213-5.

Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding and Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate.

Hashimoto Y, Taniguchi M, Uesaka K, Nomura T, Hirakawa H, Tanimoto K Front Microbiol. 2019; 10:2568.

PMID: 31798546 PMC: 6863802. DOI: 10.3389/fmicb.2019.02568.

Strategies and approaches in plasmidome studies-uncovering plasmid diversity disregarding of linear elements?.

Dib J, Wagenknecht M, Farias M, Meinhardt F Front Microbiol. 2015; 6:463.

PMID: 26074886 PMC: 4443254. DOI: 10.3389/fmicb.2015.00463.

Autotrophic, sulfur-oxidizing actinobacteria in acidic environments.

Norris P, Davis-Belmar C, Brown C, Calvo-Bado L Extremophiles. 2011; 15(2):155-63.

PMID: 21308384 DOI: 10.1007/s00792-011-0358-3.