Evaluation of Wedged Arterial Injection As a New Technique for Delivery of Experimental Therapeutic Substances into the Porcine Pancreas

Overview

Journal Exp Diabetes Res

Specialty Endocrinology

Date 2011 Oct 19

PMID 22007190

Citations 1

Authors

Rafael Latorre

Wendy Hernandez

Fei Sun

Francisco Sanchez-Margallo

Francisco Gil

Octavio Lopez-Albors

Jose M Vazquez

Jesus Uson

Affiliations

Soon will be listed here.

Abstract

Objectives: To prospectively evaluate the technical feasibility and efficacy of wedged arterial injection (WAI) as a potential route for experimental selective therapy to the pancreas of healthy pigs.

Materials And Methods: Selective angiographies were completed in ten pigs under general anaesthesia. By superselective angiography, the catheter was inserted and wedged into the major pancreatic artery, blocking the blood flow. In order to evaluate the efficacy of the WAI method, a DNA-specific fluorescent dye (Hoechst 33258) was used.

Results: Histological study revealed a uniform distribution of the fluorescent dye within the nuclei of the endocrine and exocrine pancreatic cells. Pancreatic and liver enzymes as well as histopathology of the pancreas were normal.

Conclusion: WAI is a highly effective minimally invasive methodology to target the porcine pancreas. The findings suggest that WAI may contribute to developing preclinical assays of pancreas gene or cell-transfer therapies in swine model.

Citing Articles

Evidences supporting the vascular etiology of post-double balloon enteroscopy pancreatitis: Study in porcine model.

Latorre R, Lopez-Albors O, Soria F, Morcillo E, Esteban P, Perez-Cuadrado-Robles E World J Gastroenterol. 2017; 23(34):6201-6211.

PMID: 28974886 PMC: 5603486. DOI: 10.3748/wjg.v23.i34.6201.

References

Ayuso E, Chillon M, Agudo J, Haurigot V, Bosch A, Carretero A . In vivo gene transfer to pancreatic beta cells by systemic delivery of adenoviral vectors. Hum Gene Ther. 2004; 15(8):805-12. DOI: 10.1089/1043034041648426. View

Tal M, Hirshberg B, Neeman Z, Bunnell D, Soleimanpour S, Bacher J . Induction of diabetes in nonhuman primates by means of temporary arterial embolization and selective arterial injection of streptozotocin. Radiology. 2003; 230(1):163-8. DOI: 10.1148/radiol.2301021413. View

Trotter M, Olive P, Chaplin D . Effect of vascular marker Hoechst 33342 on tumour perfusion and cardiovascular function in the mouse. Br J Cancer. 1990; 62(6):903-8. PMC: 1971547. DOI: 10.1038/bjc.1990.406. View

Loeffler D, Keng P, Wilson K, Lord E . Comparison of fluorescence intensity of Hoechst 33342-stained EMT6 tumour cells and tumour-infiltrating host cells. Br J Cancer. 1987; 56(5):571-6. PMC: 2001896. DOI: 10.1038/bjc.1987.243. View

Wang A, Peng P, Ehrhardt A, Storm T, Kay M . Comparison of adenoviral and adeno-associated viral vectors for pancreatic gene delivery in vivo. Hum Gene Ther. 2004; 15(4):405-13. DOI: 10.1089/104303404322959551. View

Siemann D, Keng P . Characterization of radiation resistant hypoxic cell subpopulations in KHT sarcomas. (II). Cell sorting. Br J Cancer. 1988; 58(3):296-300. PMC: 2246598. DOI: 10.1038/bjc.1988.207. View

Teramura Y, Iwata H . Bioartificial pancreas microencapsulation and conformal coating of islet of Langerhans. Adv Drug Deliv Rev. 2010; 62(7-8):827-40. DOI: 10.1016/j.addr.2010.01.005. View

Ferrer J, Scott 3rd W, Weegman B, Suszynski T, Sutherland D, Hering B . Pig pancreas anatomy: implications for pancreas procurement, preservation, and islet isolation. Transplantation. 2008; 86(11):1503-10. PMC: 2704055. DOI: 10.1097/TP.0b013e31818bfda1. View

Zimmermann F, Pistorius G, Grabowsky K, Motsch J, Marzi I . Pancreatic autotransplantation in the pig: variations in epigastric arterial blood supply. Transpl Int. 1989; 2(4):193-8. DOI: 10.1007/BF02414533. View

10.

Young S, Hill R . Radiation sensitivity of tumour cells stained in vitro or in vivo with the bisbenzimide fluorochrome Hoechst 33342. Br J Cancer. 1989; 60(5):715-21. PMC: 2247311. DOI: 10.1038/bjc.1989.346. View

11.

Troisi R, Jacobs B, Berrevoet F, Vereycken R, de Hemptinne B, Hesse U . The role of hepato-coeliac arterial reconstruction in porcine segmental pancreatic autotransplantation. Transplant Proc. 1998; 29(8):3625-6. DOI: 10.1016/s0041-1345(97)01049-x. View

12.

Ayuso E, Chillon M, Garcia F, Agudo J, Andaluz A, Carretero A . In vivo gene transfer to healthy and diabetic canine pancreas. Mol Ther. 2005; 13(4):747-55. DOI: 10.1016/j.ymthe.2005.10.017. View

13.

Homma H, Doi T, Mezawa S, Takada K, Kukitsu T, Oku T . A novel arterial infusion chemotherapy for the treatment of patients with advanced pancreatic carcinoma after vascular supply distribution via superselective embolization. Cancer. 2000; 89(2):303-13. View

14.

Shokouh-Amiri M, Andersen H . Segmental pancreatic autotransplantation in the pig. Transplantation. 1989; 47(1):42-4. DOI: 10.1097/00007890-198901000-00010. View

15.

Bottino R, Trucco M . Multifaceted therapeutic approaches for a multigenic disease. Diabetes. 2005; 54 Suppl 2:S79-86. DOI: 10.2337/diabetes.54.suppl_2.s79. View

16.

Chaplin L, Cohen A, Huettl P, Kennedy M, Njus D, Temperley S . Reserpic acid as an inhibitor of norepinephrine transport into chromaffin vesicle ghosts. J Biol Chem. 1985; 260(20):10981-5. View

17.

Hirshberg B, Montgomery S, Wysoki M, Xu H, Tadaki D, Lee J . Pancreatic islet transplantation using the nonhuman primate (rhesus) model predicts that the portal vein is superior to the celiac artery as the islet infusion site. Diabetes. 2002; 51(7):2135-40. DOI: 10.2337/diabetes.51.7.2135. View

18.

Camp E, Yang A, Liu W, Fan F, Somcio R, Hicklin D . Roles of nitric oxide synthase inhibition and vascular endothelial growth factor receptor-2 inhibition on vascular morphology and function in an in vivo model of pancreatic cancer. Clin Cancer Res. 2006; 12(8):2628-33. DOI: 10.1158/1078-0432.CCR-05-2257. View

19.

Traverso L, Macfarlane S . Pancreas autotransplantation--unsuitability of the swine as a model. Transplantation. 1987; 44(3):450-1. DOI: 10.1097/00007890-198709000-00026. View

20.

Loiler S, Tang Q, Clarke T, Campbell-Thompson M, Chiodo V, Hauswirth W . Localized gene expression following administration of adeno-associated viral vectors via pancreatic ducts. Mol Ther. 2005; 12(3):519-27. DOI: 10.1016/j.ymthe.2005.04.017. View