Molecular Characterization of Trimethoprim Resistance in Shigella Sonnei in Sicily

Overview

Journal Epidemiol Infect

Publisher Cambridge University Press

Specialties Infectious Diseases
Public Health

Date 1990 Aug 1

PMID 2200699

Citations 2

Authors

A Agodi

C Jones

E J Threlfall

M DAngelo

M Marranzano

Affiliations

Soon will be listed here.

Abstract

During the 3-year period 1985-7, all strains of Shigella sonnei isolated in Catania, Sicily, showed a high level of resistance to trimethoprim (Tp) which was invariably associated with resistance to other antibiotics. Plasmid analysis showed 18 different electropherotypes: 35 of 37 strains harboured a plasmid of 70 Megadaltons (MDa), and 29 of 37 strains a plasmid of 130 MDa. Restriction endonuclease fingerprinting of purified 70 MDa plasmid DNA from different strains demonstrated that these plasmids were similar but not identical. In some strains with transferable Tp resistance, DNA hybridization analysis demonstrated the presence of gene coding for the production of dihydrofolate reductase (DHFR) type V. In contrast, there was no detectable hybridization with DNA probes specific for genes coding for DHFR types I, II and IV. This is the first report of the DHFR type V gene outside Sri Lanka.

Citing Articles

Trimethoprim resistant dihydrofolate reductases in normal faecal flora isolated in India.

Tait S, Amyes S Epidemiol Infect. 1994; 113(2):247-58.

PMID: 7925663 PMC: 2271542. DOI: 10.1017/s0950268800051670.

Molecular characterization of trimethoprim resistance in salmonellas isolated in Sicily, 1985-1988.

Agodi A, Marranzano M, Jones C, Threlfall E Eur J Epidemiol. 1995; 11(1):33-8.

PMID: 7489771 DOI: 10.1007/BF01719943.

References

BAUER A, KIRBY W, SHERRIS J, Turck M . Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4):493-6. View

Watanabe T . Infective heredity of multiple drug resistance in bacteria. Bacteriol Rev. 1963; 27:87-115. PMC: 441171. DOI: 10.1128/br.27.1.87-115.1963. View

Ericsson H, SHERRIS J . Antibiotic sensitivity testing. Report of an international collaborative study. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971; 217:Suppl 217:1+. View

CHABBERT Y, Scavizzi M, Witchitz J, GERBAUD G, Bouanchaud D . Incompatibility groups and the classification of fi - resistance factors. J Bacteriol. 1972; 112(2):666-75. PMC: 251473. DOI: 10.1128/jb.112.2.666-675.1972. View

GRANT R, Bannatyne R, Shapiey A . Resistance to chloramphenicol and ampicillin of Salmonella typhimurium in Ontario, Canada. J Infect Dis. 1976; 134(4):354-61. DOI: 10.1093/infdis/134.4.354. View

Zolg J, Hanggi U, Zachau H . Isolation of a small DNA fragment carrying the gene for a dihydrofolate reductase from a trimethoprim resistance factor. Mol Gen Genet. 1978; 164(1):15-29. DOI: 10.1007/BF00267594. View

Bannatyne R, Toma S, Cheung R, Hu G . Resistance to trimethoprim and other antibiotics in Ontario shigellae. Lancet. 1980; 1(8165):425-6. DOI: 10.1016/s0140-6736(80)90977-0. View

Kado C, Liu S . Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981; 145(3):1365-73. PMC: 217141. DOI: 10.1128/jb.145.3.1365-1373.1981. View

Towner K . A clinical isolate of Escherichia coli owing its trimethoprim resistance to a chromosomally-located trimethoprim transposon. J Antimicrob Chemother. 1981; 7(2):157-62. DOI: 10.1093/jac/7.2.157. View

10.

Holmes D, Quigley M . A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981; 114(1):193-7. DOI: 10.1016/0003-2697(81)90473-5. View

11.

Broad D, Smith J . Classification of trimethoprim-resistant dihydrofolate reductases mediated by R-plasmids using isoelectric focussing. Eur J Biochem. 1982; 125(3):617-22. DOI: 10.1111/j.1432-1033.1982.tb06727.x. View

12.

Fling M, Walton L, ELWELL L . Monitoring of plasmid-encoded, trimethoprim-resistant dihydrofolate reductase genes: detection of a new resistant enzyme. Antimicrob Agents Chemother. 1982; 22(5):882-8. PMC: 185675. DOI: 10.1128/AAC.22.5.882. View

13.

Fling M, Richards C . The nucleotide sequence of the trimethoprim-resistant dihydrofolate reductase gene harbored by Tn7. Nucleic Acids Res. 1983; 11(15):5147-58. PMC: 326243. DOI: 10.1093/nar/11.15.5147. View

14.

Lopez-Brea M, Collado L, Vicente F, Perez-Diaz J . Increasing antimicrobial resistance of Shigella sonnei. J Antimicrob Chemother. 1983; 11(6):598. DOI: 10.1093/jac/11.6.598. View

15.

GROSS R, Threlfall E, Ward L, Rowe B . Drug resistance in Shigella dysenteriae, S flexneri and S boydii in England and Wales: increasing incidence of resistance to trimethoprim. Br Med J (Clin Res Ed). 1984; 288(6419):784-6. PMC: 1444629. DOI: 10.1136/bmj.288.6419.784. View

16.

Chun D, Cho D, Seol S, Suh M, Lee Y . R plasmids conferring multiple drug resistance from shigella isolated in Korea. J Hyg (Lond). 1984; 92(2):153-60. PMC: 2129251. DOI: 10.1017/s0022172400064160. View

17.

Shahid N, Rahaman M, Haider K, Banu H, Rahman N . Changing pattern of resistant Shiga bacillus (Shigella dysenteriae type 1) and Shigella flexneri in Bangladesh. J Infect Dis. 1985; 152(6):1114-9. DOI: 10.1093/infdis/152.6.1114. View

18.

Young H, Amyes S . Characterisation of a new transposon-mediated trimethoprim-resistant dihydrofolate reductase. Biochem Pharmacol. 1985; 34(24):4334-7. DOI: 10.1016/0006-2952(85)90296-5. View

19.

Young H, Amyes S . A new mechanism of plasmid trimethoprim resistance. Characterization of an inducible dihydrofolate reductase. J Biol Chem. 1986; 261(6):2503-5. View

20.

Sundstrom L, Vinayagamoorthy T, SKOLD O . Novel type of plasmid-borne resistance to trimethoprim. Antimicrob Agents Chemother. 1987; 31(1):60-6. PMC: 174652. DOI: 10.1128/AAC.31.1.60. View