Alpha 2.0
Login Register
» Articles » PMID: 2199490

Evaluation of a Human Immunodeficiency Virus Test Algorithm Utilizing a Recombinant Protein Enzyme Immunoassay

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1990 Jun 1
PMID 2199490
Citations 6
Authors
D G Lepine
P W Neumann
S L Frenette
M V OShaughnessy
Affiliations
Soon will be listed here.
Abstract

Many truly human immunodeficiency virus (HIV) antibody-negative serum samples may be unnecessarily subjected to costly and time-consuming Western blots (immunoblots). An investigation was undertaken to evaluate the efficiency of using a recombinant protein-based enzyme immunosorbent assay (EIA; Cambridge BioScience [CBC] Recombigen HIV EIA) as an adjunct to whole viral lysate EIA. A total of 2,212 serum samples which had been screened by viral lysate EIA were tested by CBC EIA in parallel with the Western blot. The sensitivity and specificity of the CBC kit were 99.9 and 99.7%, respectively. Positive and negative predictive values were 99.7 and 99.9%, respectively. The high sensitivity of this kit and its high negative predictive value make it an attractive addition to an HIV testing algorithm by reducing the number of Western blot tests on truly antibody negative serum samples.

Citing Articles

Combining pooling and alternative algorithms in seroprevalence studies.

Raboud J, Sherlock C, Schechter M, Lepine D, OShaughnessy M J Clin Microbiol. 1993; 31(9):2298-302.

PMID: 8408546 PMC: 265750. DOI: 10.1128/jcm.31.9.2298-2302.1993.

Utility of quantitative enzyme immunoassay reactivity for predicting human immunodeficiency virus seropositivity in low- and high-prevalence populations.

Hou X, Breese P, Douglas Jr J J Clin Microbiol. 1994; 32(1):220-3.

PMID: 8126185 PMC: 263002. DOI: 10.1128/jcm.32.1.220-223.1994.

Predicting human immunodeficiency virus type 1-positive sera by using two enzyme immunoassay kits in a parallel testing format.

Fonseca K, Anand C J Clin Microbiol. 1991; 29(11):2507-12.

PMID: 1774256 PMC: 270363. DOI: 10.1128/jcm.29.11.2507-2512.1991.

Detection of human immunodeficiency virus type 1 antibody by using commercially available whole-cell viral lysate, synthetic peptide, and recombinant protein enzyme immunoassay systems.

Johnson J J Clin Microbiol. 1992; 30(1):216-8.

PMID: 1734055 PMC: 265024. DOI: 10.1128/jcm.30.1.216-218.1992.

Expression in Escherichia coli and sequencing of the coding region for the capsid protein of Dutch maedi-visna virus strain ZZV 1050: application of recombinant protein in enzyme-linked immunosorbent assay for the detection of caprine and ovine....

Zanoni R, Nauta I, Pauli U, Peterhans E J Clin Microbiol. 1991; 29(7):1290-4.

PMID: 1653261 PMC: 270102. DOI: 10.1128/jcm.29.7.1290-1294.1991.

References
1.
Mortimer J . The influence of assay sensitivity and specificity on error rates in three anti-HIV testing strategies. AIDS. 1989; 3(4):199-207. DOI: 10.1097/00002030-198904000-00002. View
2.
Ransohoff D, Feinstein A . Problems of spectrum and bias in evaluating the efficacy of diagnostic tests. N Engl J Med. 1978; 299(17):926-30. DOI: 10.1056/NEJM197810262991705. View
3.
Schupbach J, Popovic M, Gilden R, Gonda M, Sarngadharan M, Gallo R . Serological analysis of a subgroup of human T-lymphotropic retroviruses (HTLV-III) associated with AIDS. Science. 1984; 224(4648):503-5. DOI: 10.1126/science.6200937. View
4.
Shaw G, Hahn B, Arya S, Groopman J, Gallo R, Wong-Staal F . Molecular characterization of human T-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome. Science. 1984; 226(4679):1165-71. DOI: 10.1126/science.6095449. View
5.
Carlson J, Bryant M, Hinrichs S, Yamamoto J, Levy N, Yee J . AIDS serology testing in low- and high-risk groups. JAMA. 1985; 253(23):3405-8. View