Anti-inflammatory Effect of Myristicin on RAW 264.7 Macrophages Stimulated with Polyinosinic-polycytidylic Acid

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2011 Oct 14

PMID 21991618

Citations 44

Authors

Ji Young Lee

Wansu Park

Affiliations

Soon will be listed here.

Abstract

Myristicin (1-allyl-5-methoxy-3,4-methylenedioxybenzene) is an active aromatic compound found in nutmeg (the seed of Myristica fragrans), carrot, basil,cinnamon, and parsley. Myristicin has been known to have anti-cholinergic, antibacterial,and hepatoprotective effects, however, the effects of myristicin on virus-stimulated macrophages are not fully reported. In this study, the anti-inflammatory effect of myristicin on double-stranded RNA (dsRNA)-stimulated macrophages was examined. Myristicin did not reduce the cell viability of RAW 264.7 mouse macrophages at concentrations of up to 50 μM. Myristicin significantly inhibited the production of calcium, nitric oxide (NO),interleukin (IL)-6, IL-10, interferon inducible protein-10, monocyte chemotactic protein(MCP)-1, MCP-3, granulocyte-macrophage colony-stimulating factor, macrophage inflammatory protein (MIP)-1α, MIP-1β, and leukemia inhibitory factor in dsRNA[polyinosinic-polycytidylic acid]-induced RAW 264.7 cells (P < 0.05). In conclusion,myristicin has anti-inflammatory properties related with its inhibition of NO, cytokines,chemokines, and growth factors in dsRNA-stimulated macrophages via the calcium pathway.

Citing Articles

Bioferments Obtained by Fermentation with Kombucha as an Important Source of Active Substances for Skin Care.

Niziol-Lukaszewska Z, Ziemlewska A, Zagorska-Dziok M, Mokrzynska A, Wojciak M, Sowa I Molecules. 2025; 30(5).

PMID: 40076207 PMC: 11902125. DOI: 10.3390/molecules30050983.

Renal health benefits and therapeutic effects of parsley (): a review.

Alobaidi S Front Med (Lausanne). 2024; 11:1494740.

PMID: 39735703 PMC: 11672790. DOI: 10.3389/fmed.2024.1494740.

Anti-inflammatory effects of kawakawa (): An integrative mRNA-miRNA approach.

Tautuiaki S, Gojer J, Jayaprakash R, Sharma P, Pook C, Foster M Food Sci Nutr. 2024; 12(11):8858-8869.

PMID: 39619950 PMC: 11606859. DOI: 10.1002/fsn3.4450.

Nutmeg Poisoning With Electrolyte Abnormalities: A Case Report.

Ichibayashi R, Sato T, Ohashi R, Konishi K Cureus. 2024; 16(10):e71840.

PMID: 39559662 PMC: 11571034. DOI: 10.7759/cureus.71840.

Houtt. methanol extract as a promising treatment for infection in experimentally immunosuppressed and immunocompetent mice.

El Shanawany E, Abouelmagd F, Taha N, Zalat R, Abdelrahman E, Abdel-Rahman E Vet World. 2024; 17(9):2062-2071.

PMID: 39507782 PMC: 11536736. DOI: 10.14202/vetworld.2024.2062-2071.

References

Ware C . Network communications: lymphotoxins, LIGHT, and TNF. Annu Rev Immunol. 2005; 23:787-819. DOI: 10.1146/annurev.immunol.23.021704.115719. View

Jiang W, Bell C, Pisetsky D . The relationship between apoptosis and high-mobility group protein 1 release from murine macrophages stimulated with lipopolysaccharide or polyinosinic-polycytidylic acid. J Immunol. 2007; 178(10):6495-503. DOI: 10.4049/jimmunol.178.10.6495. View

White K, Ding Q, Moore B, Peters-Golden M, Ware L, Matthay M . Prostaglandin E2 mediates IL-1beta-related fibroblast mitogenic effects in acute lung injury through differential utilization of prostanoid receptors. J Immunol. 2007; 180(1):637-46. PMC: 2789491. DOI: 10.4049/jimmunol.180.1.637. View

Zheng G, Kenney P, Zhang J, Lam L . Inhibition of benzo[a]pyrene-induced tumorigenesis by myristicin, a volatile aroma constituent of parsley leaf oil. Carcinogenesis. 1992; 13(10):1921-3. DOI: 10.1093/carcin/13.10.1921. View

Yoon S, Lee Y, Park S, Kim H, Bae H, Kim H . Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J Ethnopharmacol. 2009; 125(2):286-90. DOI: 10.1016/j.jep.2009.06.027. View

Adcock I, Caramori G . Cross-talk between pro-inflammatory transcription factors and glucocorticoids. Immunol Cell Biol. 2001; 79(4):376-84. DOI: 10.1046/j.1440-1711.2001.01025.x. View

Hallstrom H, Thuvander A . Toxicological evaluation of myristicin. Nat Toxins. 1997; 5(5):186-92. DOI: 10.1002/nt.3. View

Lee B, Kim J, Jung J, Choi J, Sik Han E, Lee S . Myristicin-induced neurotoxicity in human neuroblastoma SK-N-SH cells. Toxicol Lett. 2005; 157(1):49-56. DOI: 10.1016/j.toxlet.2005.01.012. View

Martins C, Doran C, Laires A, Rueff J, Rodrigues A . Genotoxic and apoptotic activities of the food flavourings myristicin and eugenol in AA8 and XRCC1 deficient EM9 cells. Food Chem Toxicol. 2010; 49(2):385-92. DOI: 10.1016/j.fct.2010.11.013. View

10.

Willems T, Lefebvre D, Neyts J, De Clercq K . Diagnostic performance and application of two commercial cell viability assays in foot-and-mouth disease research. J Virol Methods. 2011; 173(1):108-14. DOI: 10.1016/j.jviromet.2011.01.015. View

11.

Zhang Y, Xu C, Cardell L . Long-term exposure to IL-1beta enhances Toll-IL-1 receptor-mediated inflammatory signaling in murine airway hyperresponsiveness. Eur Cytokine Netw. 2009; 20(3):148-56. DOI: 10.1684/ecn.2009.0156. View

12.

Morita T, Jinno K, Kawagishi H, Arimoto Y, Suganuma H, Inakuma T . Hepatoprotective effect of myristicin from nutmeg (Myristica fragrans) on lipopolysaccharide/d-galactosamine-induced liver injury. J Agric Food Chem. 2003; 51(6):1560-5. DOI: 10.1021/jf020946n. View

13.

Barnes P, Chung K, Page C . Inflammatory mediators of asthma: an update. Pharmacol Rev. 1998; 50(4):515-96. View

14.

Narasimhan B, Dhake A . Antibacterial principles from Myristica fragrans seeds. J Med Food. 2006; 9(3):395-9. DOI: 10.1089/jmf.2006.9.395. View

15.

Stout B, Melendez K, Seagrave J, Holtzman M, Wilson B, Xiang J . STAT1 activation causes translocation of Bax to the endoplasmic reticulum during the resolution of airway mucous cell hyperplasia by IFN-gamma. J Immunol. 2007; 178(12):8107-16. DOI: 10.4049/jimmunol.178.12.8107. View

16.

Ishihara K, Hirano T . IL-6 in autoimmune disease and chronic inflammatory proliferative disease. Cytokine Growth Factor Rev. 2002; 13(4-5):357-68. DOI: 10.1016/s1359-6101(02)00027-8. View

17.

Pereira R, Dias Teixeira K, Barreto-de-Souza V, Calegari-Silva T, De-Melo L, Soares D . Novel role for the double-stranded RNA-activated protein kinase PKR: modulation of macrophage infection by the protozoan parasite Leishmania. FASEB J. 2009; 24(2):617-26. DOI: 10.1096/fj.09-140053. View

18.

Ferret P, Soum E, Negre O, Fradelizi D . Auto-protective redox buffering systems in stimulated macrophages. BMC Immunol. 2002; 3:3. PMC: 102336. DOI: 10.1186/1471-2172-3-3. View

19.

Ahmad H, Tijerina M, Tobola A . Preferential overexpression of a class MU glutathione S-transferase subunit in mouse liver by myristicin. Biochem Biophys Res Commun. 1997; 236(3):825-8. DOI: 10.1006/bbrc.1997.7053. View

20.

Blair L, Maggi Jr L, Scarim A, Corbett J . Role of interferon regulatory factor-1 in double-stranded RNA-induced iNOS expression by mouse islets. J Biol Chem. 2001; 277(1):359-65. DOI: 10.1074/jbc.M109819200. View