The Mitotic Clb Cyclins Are Required to Alleviate HIR-mediated Repression of the Yeast Histone Genes at the G1/S Transition

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 2011 Oct 8

PMID 21978826

Citations 4

Authors

Amit Dipak Amin

Dessislava K Dimova

Monica E Ferreira

Nidhi Vishnoi

Leandria C Hancock

Mary Ann Osley

Philippe Prochasson

Affiliations

Soon will be listed here.

Abstract

The histone genes are an important group of cell cycle regulated genes whose transcription is activated during the G1/S transition and repressed in early G1, late S, and G2/M. The HIR complex, comprised of Hir1, Hir2, Hir3 and Hpc2, regulates three of the four histone gene loci. While relief of repression at the G1/S boundary involves the HIR complex, as well as other cofactors, the mechanism by which this derepression occurs remains unknown. To better understand how transcriptional repression contributes to periodic expression in the cell cycle, we sought to identify the cell cycle signals required to alleviate HIR-mediated repression of the histone genes. By measuring histone gene transcription in strains with various combinations of clb mutations, we found that the mitotic Clb1/Clb2 cyclins are required to alleviate Hir-mediated repression during the G1/S transition and that Clb2 physically interacts with the HIR complex. While the HIR complex regulates histone gene transcription in combination with two other histone H3/H4 chaperones, Asf1 and Rtt106, our data demonstrate that the mitotic Clb cyclins are necessary to specifically alleviate the repressive action of the HIR complex itself in order to allow proper expression of the histone genes in late G1/early S phase.

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