Screening and Analysis of the Multiple Absorbed Bioactive Components and Metabolites of Baihe Zhimu Tang by the Metabolic Fingerprinting Technique and Liquid Chromatography/diode Array Detection-electrospray Ionization-mass Spectrometry

Overview

Journal Pharmacogn Mag

Publisher Sage Publications

Date 2011 Oct 5

PMID 21969787

Citations 3

Authors

Kunming Qin

Hao Cai

Xiao Liu

Tulin Lu

Qianbo Fang

Zhongqing Yao

Zisheng Xu

Baochang Cai

Affiliations

Soon will be listed here.

Abstract

Background: Baihe Zhimu Tang (BZT) is a widely used traditional Chinese medicinal formula in treating various diseases; however, its active components have remained unknown.

Materials And Methods: Based on the metabolic fingerprinting technique and liquid chromatography/diode array detection-electrospray ionization-mass spectrometry (LC/DAD-ESI-MS), a method for rapid screening and analysis of the multiple absorbed bioactive components and metabolites of an oral solution of Baihe Zhimu Tang (BZT) in rabbit plasma, urine and feces after oral administration of BZT was developed.

Results: The results obtained from a comprehensive comparative analysis of the fingerprints of the BZT and its metabolic fingerprints in rabbit biological samples indicated that 19 components in the BZT were absorbed into the rabbit's body. Both of them were tentatively identified from their MS and UV spectra and retention behaviors by comparing the results with the reported literature. In addition, only six components were found in the metabolic fingerprints, which suggested that they might be metabolites of some components in the BZT.

Conclusion: The findings demonstrated that the proposed method could be used to rapidly and simultaneously analyze and screen the multiple absorbed bioactive constituents and metabolites in a formula of traditional Chinese medicines (TCMs) by comparing and contrasting the chromatographic fingerprints with its metabolic fingerprints. This is very important not only for the pharmaceutical discovery process and the quality control of crude drugs, but also for explaining the curative mechanism of TCMs.

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References

Yang H, Zhang N, Li X, Chen J, Cai B . Structure-activity relationships of diverse annonaceous acetogenins against human tumor cells. Bioorg Med Chem Lett. 2009; 19(8):2199-202. DOI: 10.1016/j.bmcl.2009.02.105. View

Ye J, Zhang X, Dai W, Yan S, Huang H, Liang X . Chemical fingerprinting of Liuwei Dihuang Pill and simultaneous determination of its major bioactive constituents by HPLC coupled with multiple detections of DAD, ELSD and ESI-MS. J Pharm Biomed Anal. 2009; 49(3):638-45. DOI: 10.1016/j.jpba.2008.12.009. View

Li S, Li P, Sheng L, Li R, Qi L, Zhang L . Live cell extraction and HPLC-MS analysis for predicting bioactive components of traditional Chinese medicines. J Pharm Biomed Anal. 2006; 41(2):576-81. DOI: 10.1016/j.jpba.2006.01.014. View

Wang Y, Kong L, Hu L, Lei X, Yang L, Chou G . Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2007; 860(2):185-94. DOI: 10.1016/j.jchromb.2007.10.027. View

Wei Y, Li P, Shu B, Li H, Peng Y, Song Y . Analysis of chemical and metabolic components in traditional Chinese medicinal combined prescription containing Radix Salvia miltiorrhiza and Radix Panax notoginseng by LC-ESI-MS methods. Biomed Chromatogr. 2007; 21(8):797-809. DOI: 10.1002/bmc.775. View

Li C, Qi L, Li P, Wen X, Zhu Y, Liu E . Identification of metabolites of Danggui Buxue Tang in rat urine by liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2009; 23(13):1977-88. DOI: 10.1002/rcm.4110. View

Xie P, Chen S, Liang Y, Wang X, Tian R, Upton R . Chromatographic fingerprint analysis--a rational approach for quality assessment of traditional Chinese herbal medicine. J Chromatogr A. 2006; 1112(1-2):171-80. DOI: 10.1016/j.chroma.2005.12.091. View

Jiang Y, David B, Tu P, Barbin Y . Recent analytical approaches in quality control of traditional Chinese medicines--a review. Anal Chim Acta. 2009; 657(1):9-18. DOI: 10.1016/j.aca.2009.10.024. View

Liang Y, Xie P, Chan K . Quality control of herbal medicines. J Chromatogr B Analyt Technol Biomed Life Sci. 2004; 812(1-2):53-70. DOI: 10.1016/j.jchromb.2004.08.041. View

10.

Ma C, Wang L, Tang Y, Fan M, Xiao H, Huang C . Identification of major xanthones and steroidal saponins in rat urine by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry technology following oral administration of Rhizoma Anemarrhenae decoction. Biomed Chromatogr. 2008; 22(10):1066-83. DOI: 10.1002/bmc.1026. View

11.

Liang X, Jin Y, Wang Y, Jin G, Fu Q, Xiao Y . Qualitative and quantitative analysis in quality control of traditional Chinese medicines. J Chromatogr A. 2008; 1216(11):2033-44. DOI: 10.1016/j.chroma.2008.07.026. View

12.

Mao X, Kong L, Luo Q, Li X, Zou H . Screening and analysis of permeable compounds in Radix Angelica Sinensis with immobilized liposome chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2002; 779(2):331-9. DOI: 10.1016/s1570-0232(02)00403-8. View

13.

Ma C, Fan M, Tang Y, Li Z, Sun Z, Ye G . Identification of major alkaloids and steroidal saponins in rat serum by HPLC-diode array detection-MS/MS following oral administration of Huangbai-Zhimu herb-pair Extract. Biomed Chromatogr. 2008; 22(8):835-50. DOI: 10.1002/bmc.1000. View

14.

Wang X, Sun W, Sun H, Lv H, Wu Z, Wang P . Analysis of the constituents in the rat plasma after oral administration of Yin Chen Hao Tang by UPLC/Q-TOF-MS/MS. J Pharm Biomed Anal. 2008; 46(3):477-90. DOI: 10.1016/j.jpba.2007.11.014. View

15.

Homma M, Oka K, Yamada T, Niitsuma T, Ihto H, Takahashi N . A strategy for discovering biologically active compounds with high probability in traditional Chinese herb remedies: an application of saiboku-to in bronchial asthma. Anal Biochem. 1992; 202(1):179-87. DOI: 10.1016/0003-2697(92)90225-v. View

16.

Zhang X, Qi L, Yi L, Li P, Wen X, Yu Q . Screening and identification of potential bioactive components in a combined prescription of Danggui Buxue decoction using cell extraction coupled with high performance liquid chromatography. Biomed Chromatogr. 2007; 22(2):157-63. DOI: 10.1002/bmc.910. View

17.

Qin K, Fang Q, Cai H, Li W, Cai B . [High performance liquid chromatographic fingerprints of Baihe Zhimu Tang and its correlation to single herb]. Se Pu. 2010; 27(6):781-6. View

18.

Pan J, Cheng Y . Identification and analysis of absorbed and metabolic components in rat plasma after oral administration of 'Shuangdan' granule by HPLC-DAD-ESI-MS/MS. J Pharm Biomed Anal. 2006; 42(5):565-72. DOI: 10.1016/j.jpba.2006.05.006. View

19.

Wang Y, Ding C, Du K, Xiao Y, Wu C, Zhang J . Identification of active compounds and their metabolites by high-performance liquid chromatography/electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry from Xiao-xu-ming decoction (XXMD). Rapid Commun Mass Spectrom. 2009; 23(17):2724-32. DOI: 10.1002/rcm.4179. View

20.

Yang D, Cai S, Liu H, Guo X, Li C, Shang M . On-line identification of the constituents of Buyang Huanwu decoction in pig serum using combined HPLC-DAD-MS techniques. J Chromatogr B Analyt Technol Biomed Life Sci. 2006; 831(1-2):288-302. DOI: 10.1016/j.jchromb.2005.12.032. View