Probing the Interaction of Lipids with the Non-annular Binding Sites of the Potassium Channel KcsA by Magic-angle Spinning NMR

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 2011 Oct 4

PMID 21963409

Citations 18

Authors

Phedra Marius

Maurits R R de Planque

Philip T F Williamson

Affiliations

Soon will be listed here.

Abstract

The activity of the potassium channel KcsA is tightly regulated through the interactions of anionic lipids with high-affinity non-annular lipid binding sites located at the interface between the channel's subunits. Here we present solid-state phosphorous NMR studies that resolve the negatively charged lipid phosphatidylglycerol within the non-annular lipid-binding site. Perturbations in chemical shift observed upon the binding of phosphatidylglycerol are indicative of the interaction of positively charged sidechains within the non-annular binding site and the negatively charged lipid headgroup. Site directed mutagenesis studies have attributed these charge interactions to R64 and R89. Functionally the removal of the positive charges from R64 and R89 appears to act synergistically to reduce the probability of channel opening.

Citing Articles

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