The Stability of Markers in Dried-blood Spots for Recommended Newborn Screening Disorders in the United States

Overview

Journal Clin Biochem

Specialty Biochemistry

Date 2011 Oct 4

PMID 21963384

Citations 45

Authors

B W Adam

E M Hall

M Sternberg

T H Lim

S R Flores

S OBrien

D Simms

L X Li

V R De Jesus

W H Hannon

Affiliations

Soon will be listed here.

Abstract

Objective: We aimed to measure separately the contributions of heat and humidity to changes in levels of 34 markers of inborn disorders in dried-blood-spot (DBS) samples.

Design And Methods: We stored paired sets of DBSs at 37°C for predetermined intervals in low-humidity and high-humidity environments. Marker levels of all samples in each complete sample set were measured in a single analytic run.

Results: During the 30 ± 5 day studies, galactose-1-phosphate uridyltransferase and biotinidase lost almost 65% of initial activities in low-humidity storage; most of the degradation in 27 other markers was attributable to adverse effects of high-humidity storage; seven markers in DBSs stored at high humidity lost more than 90% of initial levels by the end of the study and 4 of the 7 lost more than 50% of initial levels within the first week of storage.

Conclusions: Minimizing both humidity and temperature in DBS transportation and storage environments is essential to maintaining sample integrity.

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