PRNP Genetic Variability and Molecular Typing of Natural Goat Scrapie Isolates in a High Number of Infected Flocks

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2011 Oct 4

PMID 21961834

Citations 24

Authors

Eirini G Fragkiadaki

Gabriele Vaccari

Loukia V Ekateriniadou

Umberto Agrimi

Nektarios D Giadinis

Barbara Chiappini

Elena Esposito

Michela Conte

Romolo Nonno

Affiliations

Soon will be listed here.

Abstract

One hundred and four scrapie positive and 77 negative goats from 34 Greek mixed flocks were analysed by prion protein gene sequencing and 17 caprine scrapie isolates from 11 flocks were submitted to molecular isolate typing. For the first time, the protective S146 variant was reported in Greece, while the protective K222 variant was detected in negative but also in five scrapie positive goats from heavily infected flocks. By immunoblotting six isolates, including two goat flockmates carrying the K222 variant, showed molecular features slightly different from all other Greek and Italian isolates co-analysed, possibly suggesting the presence of different scrapie strains in Greece.

Citing Articles

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National Prevalence of Caprine Prion Protein Genetic Variability at Codons 146, 211, and 222 in Goat Herds in the United States.

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Polymorphisms of Codons 110, 146, 211 and 222 at the Goat Locus and Their Association with Scrapie in Greece.

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