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Activation of T Lymphocytes in Atherosclerotic Plaques

Overview

Overview

Journal Arterioscler Thromb Vasc Biol

Date 2011 Oct 1

PMID 21960562

Citations 52

Authors

Authors

Jean-Charles Grivel

Oxana Ivanova

Natalia Pinegina

Paul S Blank

Alexander Shpektor

Leonid B Margolis

Elena Vasilieva

Affiliations

Affiliations

Soon will be listed here.

Abstract

Objective: To decipher the immunologic mechanisms of plaque maturation and rupture, it is necessary to analyze the phenotypes and distribution of individual lymphocytes that migrate to the plaques, as well as their activation at different stages of plaque formation.

Methods And Results: We developed a protocol to isolate plaque-residing immune cells and analyze their status using polychromatic flow cytometry. We found that the composition and phenotype of T lymphocytes in the plaques differs from that in blood. CD4 and, in particular, CD8(+) T cells in plaques are highly activated; the fraction of CD8 T cells coexpressing CD25 and human leukocyte antigen-D related in plaques was 6 times as large as in blood.

Conclusions: The first flow-cytoanalysis of individual T cells in atherosclerotic plaques indicates that plaques represent a separate immunologic compartment from blood with lymphocytes characterized by a high level of T-cell activation, which is compatible with the presence of antigen(s) that trigger infiltration activation of these cells. The ability to isolate and characterize these cells may lead to the identification of such antigens.

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References

1.

Niccoli G, Apa R, Lanzone A, Liuzzo G, Spaziani C, Sagnella F . CD4+CD28 null T lymphocytes are expanded in young women with polycystic ovary syndrome. Fertil Steril. 2011; 95(8):2651-4. DOI: 10.1016/j.fertnstert.2011.01.129. View

2.

Gewaltig J, Kummer M, Koella C, Cathomas G, Biedermann B . Requirements for CD8 T-cell migration into the human arterial wall. Hum Pathol. 2008; 39(12):1756-62. DOI: 10.1016/j.humpath.2008.04.018. View

3.

Arbustini E, Dal Bello B, Morbini P, Burke A, Bocciarelli M, Specchia G . Plaque erosion is a major substrate for coronary thrombosis in acute myocardial infarction. Heart. 1999; 82(3):269-72. PMC: 1729173. DOI: 10.1136/hrt.82.3.269. View

4.

Erbel C, Sato K, Meyer F, Kopecky S, Frye R, Goronzy J . Functional profile of activated dendritic cells in unstable atherosclerotic plaque. Basic Res Cardiol. 2006; 102(2):123-32. DOI: 10.1007/s00395-006-0636-x. View

5.

De Palma R, Del Galdo F, Abbate G, Chiariello M, Calabro R, Forte L . Patients with acute coronary syndrome show oligoclonal T-cell recruitment within unstable plaque: evidence for a local, intracoronary immunologic mechanism. Circulation. 2006; 113(5):640-6. DOI: 10.1161/CIRCULATIONAHA.105.537712. View

6.

Chen S, Crother T, Arditi M . Emerging role of IL-17 in atherosclerosis. J Innate Immun. 2010; 2(4):325-33. PMC: 2895754. DOI: 10.1159/000314626. View

7.

Hochman J, Tamis J, Thompson T, Weaver W, White H, Van de Werf F . Sex, clinical presentation, and outcome in patients with acute coronary syndromes. Global Use of Strategies to Open Occluded Coronary Arteries in Acute Coronary Syndromes IIb Investigators. N Engl J Med. 1999; 341(4):226-32. DOI: 10.1056/NEJM199907223410402. View

8.

Appay V, van Lier R, Sallusto F, Roederer M . Phenotype and function of human T lymphocyte subsets: consensus and issues. Cytometry A. 2008; 73(11):975-83. DOI: 10.1002/cyto.a.20643. View

9.

Libby P . Role of inflammation in atherosclerosis associated with rheumatoid arthritis. Am J Med. 2008; 121(10 Suppl 1):S21-31. DOI: 10.1016/j.amjmed.2008.06.014. View

10.

Rizzello V, Liuzzo G, Brugaletta S, Rebuzzi A, Biasucci L, Crea F . Modulation of CD4(+)CD28null T lymphocytes by tumor necrosis factor-alpha blockade in patients with unstable angina. Circulation. 2006; 113(19):2272-7. DOI: 10.1161/CIRCULATIONAHA.105.588533. View

11.

Rossmann A, Henderson B, Heidecker B, Seiler R, Fraedrich G, Singh M . T-cells from advanced atherosclerotic lesions recognize hHSP60 and have a restricted T-cell receptor repertoire. Exp Gerontol. 2008; 43(3):229-37. DOI: 10.1016/j.exger.2007.11.009. View

12.

Theodorou G, Marousi S, Ellul J, Mougiou A, Theodori E, Mouzaki A . T helper 1 (Th1)/Th2 cytokine expression shift of peripheral blood CD4+ and CD8+ T cells in patients at the post-acute phase of stroke. Clin Exp Immunol. 2008; 152(3):456-63. PMC: 2453204. DOI: 10.1111/j.1365-2249.2008.03650.x. View

13.

Libby P, Okamoto Y, Rocha V, Folco E . Inflammation in atherosclerosis: transition from theory to practice. Circ J. 2010; 74(2):213-20. DOI: 10.1253/circj.cj-09-0706. View

14.

Giubilato S, Liuzzo G, Brugaletta S, Pitocco D, Graziani F, Smaldone C . Expansion of CD4+CD28null T-lymphocytes in diabetic patients: exploring new pathogenetic mechanisms of increased cardiovascular risk in diabetes mellitus. Eur Heart J. 2011; 32(10):1214-26. DOI: 10.1093/eurheartj/ehq499. View

15.

Naruko T, Ueda M, Haze K, van der Wal A, van der Loos C, Itoh A . Neutrophil infiltration of culprit lesions in acute coronary syndromes. Circulation. 2002; 106(23):2894-900. DOI: 10.1161/01.cir.0000042674.89762.20. View

16.

Humphrey L, Fu R, Buckley D, Freeman M, Helfand M . Periodontal disease and coronary heart disease incidence: a systematic review and meta-analysis. J Gen Intern Med. 2008; 23(12):2079-86. PMC: 2596495. DOI: 10.1007/s11606-008-0787-6. View

17.

Barlic J, Murphy P . Chemokine regulation of atherosclerosis. J Leukoc Biol. 2007; 82(2):226-36. DOI: 10.1189/jlb.1206761. View

18.

Keller T, van der Meer J, Teeling P, van der Sluijs K, Idu M, Rimmelzwaan G . Selective expansion of influenza A virus-specific T cells in symptomatic human carotid artery atherosclerotic plaques. Stroke. 2007; 39(1):174-9. DOI: 10.1161/STROKEAHA.107.491282. View

19.

Shi Y, Tokunaga O . Chlamydia pneumoniae and multiple infections in the aorta contribute to atherosclerosis. Pathol Int. 2003; 52(12):755-63. DOI: 10.1046/j.1440-1827.2002.01422.x. View

20.

Liuzzo G, Biasucci L, Trotta G, Brugaletta S, Pinnelli M, Digianuario G . Unusual CD4+CD28null T lymphocytes and recurrence of acute coronary events. J Am Coll Cardiol. 2007; 50(15):1450-8. DOI: 10.1016/j.jacc.2007.06.040. View