Preclinical Pharmacokinetics and Biodistribution of Human Papillomavirus DNA Vaccine Delivered in Human Endogenous Retrovirus Envelope-coated Baculovirus Vector

Overview

Journal Pharm Res

Specialties Pharmacology
Pharmacy

Date 2011 Sep 28

PMID 21948385

Citations 4

Authors

Hee-Jeong Cho

Soondong Lee

Saewon Im

Mi-Gyeong Kim

Jaewoo Lee

Hee-Jung Lee

Keyong Ho Lee

Sujeong Kim

Young Bong Kim

Yu-Kyoung Oh

Affiliations

Soon will be listed here.

Abstract

Purpose: Test pharmacokinetics and biodistribution of a human papillomavirus(HPV)16L1 DNA vaccine delivered in human endogenous retrovirus envelope protein (HERV)-expressing baculovirus (AcHERV) and those of naked plasmid vaccine.

Method: HPV16L1 gene was administrated as a naked plasmid or in AcHERV to mice via intravenous and intramuscular routes. HPV16L1 gene was extracted and assayed by quantitative real-time polymerase chain reaction, which was determined to have a detection limit of 50 copies/µg genomic DNA..

Results: Mean residence times of HPV16L1 in AcHERV were 4.8- and 272.2-fold higher than naked HPV16L1 DNA vaccines after intramuscular and intravenous administration, respectively. Naked HPV16L1 DNA levels 1 month after injection were >3 orders of magnitude lower in each tissue tested than AcHERV-delivered HPV16L1, which was retained in most tissues without specific tissue tropism. AcHERV-delivered HPV16L1 administered intramuscularly persisted at the injection sites. However, the levels of copy numbers in muscle were low (1,800/μg genomic DNA) after 1 month, and undetectable after 6 months.

Conclusions: HPV16L1 delivered via AcHERV resides longer in the body than HPV16L1 in naked form. The lack of tissue tropism ensures the safety of AcHERV vectors for further development.

Citing Articles

Comparative Biodistribution Study of Baculoviral and Adenoviral Vector Vaccines against SARS-CoV-2.

Lee H, Chun J, Kim S, Aleksandra N, Lee C, Yoon D J Microbiol Biotechnol. 2023; 34(1):185-191.

PMID: 37830223 PMC: 10840461. DOI: 10.4014/jmb.2308.08042.

Tissue-specific Calibration of Real-time PCR Facilitates Absolute Quantification of Plasmid DNA in Biodistribution Studies.

Ho J, White P, Pouton C Mol Ther Nucleic Acids. 2016; 5(10):e371.

PMID: 27701400 PMC: 5095683. DOI: 10.1038/mtna.2016.79.

Sublingual immunization of trivalent human papillomavirus DNA vaccine in baculovirus nanovector for protection against vaginal challenge.

Lee H, Cho H, Kim M, Heo Y, Cho Y, Gwon Y PLoS One. 2015; 10(3):e0119408.

PMID: 25789464 PMC: 4366369. DOI: 10.1371/journal.pone.0119408.

Protective efficacy of a human endogenous retrovirus envelope-coated, nonreplicable, baculovirus-based hemagglutin vaccine against pandemic influenza H1N1 2009.

Choi J, Gwon Y, Kim J, Cho Y, Heo Y, Cho H PLoS One. 2013; 8(11):e80762.

PMID: 24260476 PMC: 3832454. DOI: 10.1371/journal.pone.0080762.

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