Overexpression of Receptor for Hyaluronan-mediated Motility (RHAMM) in MC3T3-E1 Cells Induces Proliferation and Differentiation Through Phosphorylation of ERK1/2

Overview

Journal J Bone Miner Metab

Specialty Endocrinology

Date 2011 Sep 28

PMID 21947782

Citations 11

Authors

Hiroko Hatano

Hideo Shigeishi

Yasusei Kudo

Koichiro Higashikawa

Kei Tobiume

Takashi Takata

Nobuyuki Kamata

Affiliations

Soon will be listed here.

Abstract

Receptor for hyaluronan (HA)-mediated motility (RHAMM) was first described as a soluble HA binding protein released by sub-confluent migrating cells. We previously found that RHAMM was highly expressed and plays an important role in proliferation in the human cementifying fibroma (HCF) cell line, which we previously established. HCF is a benign fibro-osseous neoplasm of the jaw and is composed of fibrous tissue containing varying amounts of mineralized material. However, the pathogenesis of HCF is not clear. In this paper, we examined the roles of RHAMM in osteoblastic cells. We generated RHAMM-overexpressing MC3T3-E1 cells and examined the cell proliferation and differentiation of osteoblastic cells. In MC3T3-E1 cells, overexpressing RHAMM was located intracellular and activated ERK1/2. Interestingly, the ERK1/2 activated by RHAMM overexpression promoted cell proliferation and suppressed the differentiation of osteoblastic cells. Our findings strongly suggest that RHAMM may play a key role in the osteoblastic differentiation process. The rupture of balance from differentiation to proliferation induced by RHAMM overexpression may link to the pathogenesis of bone neoplasms such as HCF.

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