In Vivo Imaging of Epileptic Activity Using 2-NBDG, a Fluorescent Deoxyglucose Analog

Overview

Journal J Neurosci Methods

Specialty Neurology

Date 2011 Sep 24

PMID 21939688

Citations 34

Authors

Vassiliy Tsytsarev

Konstantin I Maslov

Junjie Yao

Archana R Parameswar

Alexei V Demchenko

Lihong V Wang

Affiliations

Soon will be listed here.

Abstract

Accurately locating epileptic foci has great importance in advancing the treatment of epilepsy. In this study, epileptic seizures were first induced by intracortical injection of 4-aminopyridine in rats. A fluorescent deoxyglucose substitute, 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG), was then continuously injected via the tail vein. Brain glucose metabolism was subsequently monitored by fluorescence imaging of 2-NBDG. The initial uptake rate of 2-NBDG at the injection site of 4-aminopyridine significantly exceeded that of the control injection site, which indicated local hypermetabolism induced by seizures. Our results show that 2-NBDG can be used for localizing epileptic foci.

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