SAXS Study on Myoglobin Embedded in Amorphous Saccharide Matrices

Overview

Journal Eur Phys J E Soft Matter

Publisher EDP Sciences

Specialty Biophysics

Date 2011 Sep 23

PMID 21938613

Citations 3

Authors

S Giuffrida

M Panzica

F M Giordano

A Longo

Affiliations

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Abstract

We report on Small Angle X-ray Scattering (SAXS) measurements performed on samples of carboxy-myoglobin and met-myoglobin embedded in low hydrated matrices of four different saccharides (trehalose, sucrose, maltose and lactose). Results confirm the already reported occurrence of inhomogeneities, which are not peculiar of trehalose samples, but appear also in maltose and lactose, and in some cases also sucrose, being dependent on the sample hydration and on the presence of sodium dithionite. This behaviour confirms our previous interpretation about the nature of the inhomogeneities, and prompt it as a possible general behaviour for highly concentrated sugar matrices.

Citing Articles

"Water Association" Band in Saccharide Amorphous Matrices: Role of Residual Water on Bioprotection.

Giuffrida S, Cupane A, Cottone G Int J Mol Sci. 2021; 22(5).

PMID: 33801421 PMC: 7958616. DOI: 10.3390/ijms22052496.

Hydration-Induced Structural Changes in the Solid State of Protein: A SAXS/WAXS Study on Lysozyme.

Phan-Xuan T, Bogdanova E, Fureby A, Fransson J, Terry A, Kocherbitov V Mol Pharm. 2020; 17(9):3246-3258.

PMID: 32787275 PMC: 7482395. DOI: 10.1021/acs.molpharmaceut.0c00351.

Proteins in amorphous saccharide matrices: structural and dynamical insights on bioprotection.

Giuffrida S, Cottone G, Bellavia G, Cordone L Eur Phys J E Soft Matter. 2013; 36(7):79.

PMID: 23884626 DOI: 10.1140/epje/i2013-13079-x.

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