Lipoprint Adequately Estimates LDL Size Distribution, but Not Absolute Size, Versus Polyacrylamide Gradient Gel Electrophoresis

Overview

Journal Lipids

Specialty Biochemistry

Date 2011 Sep 22

PMID 21935654

Citations 11

Authors

Krista A Varady

Benoit Lamarche

Affiliations

Soon will be listed here.

Abstract

Recently, a new cost-effective and less labor-intensive technique termed the "lipoprint LDL system" was developed to measure LDL particle size. However, the agreement between lipoprint and previously validated techniques, such as polyacrylamide gradient gel electrophoresis (PGGE), has never been tested. Therefore, we measured LDL size by lipoprint and PGGE in 16 obese subjects at 4 different time points. Lipoprint significantly overestimated (P = 0.003) integrated LDL particle size by 1.1 ± 3.0 Å when compared to PGGE. As for distribution, there was good agreement between methods for the estimation of large, medium, and small particles (mean difference between the methods was <3% for each parameter). Correlational analysis also revealed good relationships between methods for the proportion of large (r = 0.81, P < 0.0001), medium (r = 0.67, P < 0.0001), and small (r = 0.73, P < 0.0001) particles. In sum, although there is good agreement between lipoprint and PGGE for the determination of LDL size distribution, absolute LDL size values may differ between the two methods.

Citing Articles

Review of Laboratory Methods to Determine HDL and LDL Subclasses and Their Clinical Importance.

Chary A, Hedayati M Rev Cardiovasc Med. 2024; 23(4):147.

PMID: 39076233 PMC: 11273998. DOI: 10.31083/j.rcm2304147.

The Oxidized Lipoproteins In Vivo: Its Diversity and Behavior in the Human Circulation.

Itabe H, Obama T Int J Mol Sci. 2023; 24(6).

PMID: 36982815 PMC: 10053446. DOI: 10.3390/ijms24065747.

Changes in lipoproteins associated with lipid-lowering and antiplatelet strategies in patients with acute myocardial infarction.

Lotfollahi Z, Mello A, Fonseca F, Machado L, Mathias A, Izar M PLoS One. 2022; 17(8):e0273292.

PMID: 36040917 PMC: 9426937. DOI: 10.1371/journal.pone.0273292.

Anorexia Nervosa Is Associated with a Shift to Pro-Atherogenic Low-Density Lipoprotein Subclasses.

Stadler J, Lackner S, Morkl S, Meier-Allard N, Scharnagl H, Rani A Biomedicines. 2022; 10(4).

PMID: 35453644 PMC: 9030549. DOI: 10.3390/biomedicines10040895.

Association between small dense low-density lipoprotein cholesterol and neuroimaging markers of cerebral small vessel disease in middle-aged and elderly Chinese populations.

Yu X, Yu Y, Wei C, Wang L, Jiang J, Zhang R BMC Neurol. 2021; 21(1):436.

PMID: 34753453 PMC: 8576978. DOI: 10.1186/s12883-021-02472-6.

References

Bland J, Altman D . Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986; 1(8476):307-10. View

Varady K, Bhutani S, Church E, Klempel M . Short-term modified alternate-day fasting: a novel dietary strategy for weight loss and cardioprotection in obese adults. Am J Clin Nutr. 2009; 90(5):1138-43. DOI: 10.3945/ajcn.2009.28380. View

Miljkovic-Gacic I, Bunker C, Ferrell R, Kammerer C, Evans R, Patrick A . Lipoprotein subclass and particle size differences in Afro-Caribbeans, African Americans, and white Americans: associations with hepatic lipase gene variation. Metabolism. 2005; 55(1):96-102. DOI: 10.1016/j.metabol.2005.07.011. View

Rizzo M, Pernice V, Frasheri A, Di Lorenzo G, Rini G, Spinas G . Small, dense low-density lipoproteins (LDL) are predictors of cardio- and cerebro-vascular events in subjects with the metabolic syndrome. Clin Endocrinol (Oxf). 2008; 70(6):870-5. DOI: 10.1111/j.1365-2265.2008.03407.x. View

Hoefner D, Hodel S, OBrien J, Branum E, Sun D, Meissner I . Development of a rapid, quantitative method for LDL subfractionation with use of the Quantimetrix Lipoprint LDL System. Clin Chem. 2001; 47(2):266-74. View

Tchernof A, Lamarche B, Prudhomme D, Nadeau A, Moorjani S, Labrie F . The dense LDL phenotype. Association with plasma lipoprotein levels, visceral obesity, and hyperinsulinemia in men. Diabetes Care. 1996; 19(6):629-37. DOI: 10.2337/diacare.19.6.629. View

Berneis K, Rizzo M, Spinas G, Di Lorenzo G, Di Fede G, Pepe I . The predictive role of atherogenic dyslipidemia in subjects with non-coronary atherosclerosis. Clin Chim Acta. 2009; 406(1-2):36-40. DOI: 10.1016/j.cca.2009.05.002. View

St-Pierre A, Ruel I, Cantin B, Dagenais G, BERNARD P, Despres J . Comparison of various electrophoretic characteristics of LDL particles and their relationship to the risk of ischemic heart disease. Circulation. 2001; 104(19):2295-9. DOI: 10.1161/hc4401.098490. View

Apostolou F, Gazi I, Kostoula A, Tellis C, Tselepis A, Elisaf M . Persistence of an atherogenic lipid profile after treatment of acute infection with Brucella. J Lipid Res. 2009; 50(12):2532-9. PMC: 2781324. DOI: 10.1194/jlr.P900063-JLR200. View

10.

Abbas J, Chakraborty J, Akanji A, Doi S . Hypothyroidism results in small dense LDL independent of IRS traits and hypertriglyceridemia. Endocr J. 2008; 55(2):381-9. DOI: 10.1507/endocrj.k07e-065. View

11.

Lagos K, Filippatos T, Tsimihodimos V, Gazi I, Rizos C, Tselepis A . Alterations in the high density lipoprotein phenotype and HDL-associated enzymes in subjects with metabolic syndrome. Lipids. 2008; 44(1):9-16. DOI: 10.1007/s11745-008-3251-9. View

12.

Rizzo M, Berneis K . Low-density lipoprotein size and cardiovascular risk assessment. QJM. 2005; 99(1):1-14. DOI: 10.1093/qjmed/hci154. View

13.

Ensign W, Hill N, Heward C . Disparate LDL phenotypic classification among 4 different methods assessing LDL particle characteristics. Clin Chem. 2006; 52(9):1722-7. DOI: 10.1373/clinchem.2005.059949. View

14.

Fernandez M, Jones J, Ackerman D, Barona J, Calle M, Comperatore M . Low HDL cholesterol is associated with increased atherogenic lipoproteins and insulin resistance in women classified with metabolic syndrome. Nutr Res Pract. 2011; 4(6):492-8. PMC: 3029790. DOI: 10.4162/nrp.2010.4.6.492. View

15.

Superko H, Gadesam R . Is it LDL particle size or number that correlates with risk for cardiovascular disease?. Curr Atheroscler Rep. 2008; 10(5):377-85. DOI: 10.1007/s11883-008-0059-2. View

16.

Norata G, Raselli S, Grigore L, Garlaschelli K, Vianello D, Bertocco S . Small dense LDL and VLDL predict common carotid artery IMT and elicit an inflammatory response in peripheral blood mononuclear and endothelial cells. Atherosclerosis. 2009; 206(2):556-62. DOI: 10.1016/j.atherosclerosis.2009.03.017. View

17.

Krauss R . Lipoprotein subfractions and cardiovascular disease risk. Curr Opin Lipidol. 2010; 21(4):305-11. DOI: 10.1097/MOL.0b013e32833b7756. View

18.

Hirany S, Othman Y, Kutscher P, Rainwater D, Jialal I, Devaraj S . Comparison of low-density lipoprotein size by polyacrylamide tube gel electrophoresis and polyacrylamide gradient gel electrophoresis. Am J Clin Pathol. 2003; 119(3):439-45. DOI: 10.1309/h4e6ktyufff23hfn. View