» Articles » PMID: 21933867

Estimation of Estradiol in Mouse Serum Samples: Evaluation of Commercial Estradiol Immunoassays

Overview
Journal Endocrinology
Specialty Endocrinology
Date 2011 Sep 22
PMID 21933867
Citations 78
Authors
Affiliations
Soon will be listed here.
Abstract

The University of Virginia Center for Research in Reproduction Ligand Core performed an evaluation of nine commercial estradiol (E2) immunoassays for use with mouse serum. The evaluation had two components. 1) Recovery Studies: a mouse pool was spiked with E2 concentrations across the assay range, and percent recovery and parallelism to the assay standard curve were determined. 2) Correlation Studies: serum pools were collected from intact females, ovariectomized (OVX) and OVX-E2 treated mice and E2 assayed, then measured by gas chromatography/tandem mass spectrometry (GC/MSMS) for comparison to a gold standard method. Recovery results showed that E2 recovery from spiked mouse pools varied greatly (from <18% to >640%) among kits tested. However, three kits (DiaSorin Radioimmunoassay, Siemens Double Antibody RIA, and CalBiotech Enzyme Immunoassay) showed reasonable recoveries and parallelism. Data collected from the Correlation Study showed that values from intact, OVX and OVX-E2-treated mouse pools varied by several fold vs. GC/MSMS for most of the kits tested. The DiaSorin RIA and CalBiotech Enzyme Immunoassay Kits showed the best correlation to GC/MSMS. Unfortunately, while this evaluation was ongoing, the DiaSorin Kit was discontinued. In summary, the CalBiotech Kit was the only available assay tested that demonstrated good E2 parallelism to the assay standard curve and accuracy vs. a gold standard method (i.e. GC/MSMS). Also of note, the CalBiotech assay is sensitive and requires minimal sample volume. Therefore, based on these findings the CalBiotech E2 assay has been implemented for use in mouse serum samples within the Ligand Core.

Citing Articles

In Vitro Assay to Examine Osteoclast Resorptive Activity Under Estrogen Withdrawal.

Fiorino C, Omer S, Gandhi N, Harrison R Bio Protoc. 2025; 15(1):e5155.

PMID: 39803321 PMC: 11717722. DOI: 10.21769/BioProtoc.5155.


In Vitro Cell Culture Model for Osteoclast Activation during Estrogen Withdrawal.

Gandhi N, Omer S, Harrison R Int J Mol Sci. 2024; 25(11).

PMID: 38892322 PMC: 11173070. DOI: 10.3390/ijms25116134.


Gynecological surgery in adulthood imparts cognitive and brain changes in rats: A focus on hysterectomy at short-, moderate-, and long-term intervals after surgery.

Koebele S, Bernaud V, Northup-Smith S, Willeman M, Strouse I, Bulen H Horm Behav. 2023; 155:105411.

PMID: 37659358 PMC: 11060888. DOI: 10.1016/j.yhbeh.2023.105411.


Cellular and extracellular vaginal changes following murine ovarian removal.

McCracken J, Calderon G, Le Q, Faruqui N, Balaji S, Hakim J Physiol Rep. 2023; 11(15):e15762.

PMID: 37549960 PMC: 10406565. DOI: 10.14814/phy2.15762.


Altered GnRH neuron and ovarian innervation characterize reproductive dysfunction linked to the Fragile X messenger ribonucleoprotein () gene mutation.

Villa P, Lainez N, Jonak C, Berlin S, Ethell I, Coss D Front Endocrinol (Lausanne). 2023; 14:1129534.

PMID: 36909303 PMC: 9992745. DOI: 10.3389/fendo.2023.1129534.


References
1.
Carlstrom K . Low endogenous estrogen levels--analytical problems and tissue sensitivity. Acta Obstet Gynecol Scand Suppl. 1996; 163:11-5. View

2.
Miller J, Valdes Jr R . Approaches to minimizing interference by cross-reacting molecules in immunoassays. Clin Chem. 1991; 37(2):144-53. View

3.
Dehennin L . Estradiol-17 beta determined in plasma by gas chromatography-mass spectrometry with selected ion monitoring of mixed silyl ether-perfluoroacyl ester derivatives and use of various stable-isotope-labeled internal standards. Clin Chem. 1989; 35(4):532-6. View

4.
Nelson R, Grebe S, OKane D, Singh R . Liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of estradiol and estrone in human plasma. Clin Chem. 2003; 50(2):373-84. DOI: 10.1373/clinchem.2003.025478. View

5.
Pratt J, Koops W, WOLDRING M, Wiegman T . Specificity of immunoassays. I. Effect of plasma proteins on the specificity of steroid immunoassay. Eur J Nucl Med. 1976; 1(1):37-48. DOI: 10.1007/BF00253265. View