Surveillance of Hepatitis A and E Viruses Contamination in Shellfish in Thailand

Overview

Journal Lett Appl Microbiol

Publisher Oxford University Press

Specialty Microbiology

Date 2011 Sep 21

PMID 21929540

Citations 16

Authors

A Namsai

S Louisirirotchanakul

N Wongchinda

U Siripanyaphinyo

P Virulhakul

P Puthavathana

K S Myint

M Gannarong

R Ittapong

Affiliations

Soon will be listed here.

Abstract

Aims: To survey for hepatitis A virus (HAV) and hepatitis E virus (HEV) contamination in edible bivalve shellfish.

Methods And Results: A total of 213 shellfish (52 oysters, 69 cockles and 92 mussels) collected from a culture farm and two retailed markets were investigated for HAV and HEV contamination by reverse transcription-polymerase chain reaction (RT-PCR) assay using HA2-HA1 (capsid region) and HE366-HE363 (ORF2/3 overlapping region) primers, respectively. It was found that 3.8% of the shellfish and 2.9 and 6.5% of the cockle and mussel, respectively, showed positive for HAV detection. Nucleotide sequencing of all the 8 HAV-positive shellfish revealed 97-100% similarity to HAV subgenotype IA. Interestingly, viruses were found more frequently in the gills than in digestive tissue (4.5%vs 0.5%, P = 0.045). All the shellfish were negative for HEV.

Conclusion: Significant contamination of HAV in edible bivalve shellfish was observed. Beside digestive tissue, gills are one of the important samples for viral genome detection.

Significance And Impact Of The Study: HAV-contaminated shellfish can play a role as reservoirs and/or vehicles in faecal-oral transmission in Thailand, and further monitoring of such a contamination is required.

Citing Articles

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Hepatitis a Vaccine as Opportunity of Primary Prevention for Food Handlers: A Narrative Review.

Fallucca A, Restivo V, Sgariglia M, Roveta M, Trucchi C Vaccines (Basel). 2023; 11(7).

PMID: 37515087 PMC: 10383099. DOI: 10.3390/vaccines11071271.

Hepatitis A and E Viruses in Mussels from Cherrat Estuary in Morocco: Detection by Real-Time Reverse Transcription PCR Analysis.

Bazir H, Hassou N, El Mellouli F, Zekhnini H, Najib S, Ennaji M Adv Virol. 2022; 2022:8066356.

PMID: 36479562 PMC: 9722280. DOI: 10.1155/2022/8066356.

A case-control study of acute hepatitis A in South Korea, 2019.

Hyun J, Yoon J, Lee S Osong Public Health Res Perspect. 2022; 13(5):352-359.

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Hepatitis E Virus Seroprevalence and Associated Risk Factors in Pregnant Women Attending Antenatal Consultations in Senegal.

Diouara A, Lo S, Nguer C, Senghor A, Diop Ndiaye H, Manga N Viruses. 2022; 14(8).

PMID: 36016364 PMC: 9416362. DOI: 10.3390/v14081742.