Immunohistochemical Study of Pre-S(2) Antigen in Liver and Serum of Patients with Chronic Hepatitis B

Overview

Journal Gastroenterol Jpn

Specialty Gastroenterology

Date 1990 Jun 1

PMID 2192932

Citations 1

Authors

K Takaguchi

G Yamada

T Tsuji

Affiliations

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Abstract

To evaluate the significance of pre-S(2) in the liver, the authors studied the relationship between serum and intrahepatic pre-S(2) in 27 patients with chronic hepatitis B. Intrahepatic expression of pre-S(2) was observed under light and electron microscopy and compared with that of HBsAg and HBcAg. All patients were positive for hepatic pre-S(2), and 25 for serum pre-S(2). Intrahepatic pre-S(2) expression was membranous or both membranous and cytoplasmic in 18 cases and was cytoplasmic in 9. The serum levels of pre-S(2) were significantly higher in cases whose intrahepatic pre-S(2) was membranous than in the cytoplasmic cases. These findings indicate that membranous expression of pre-S(2) reflects its secretion into the circulation. Intrahepatic pre-S(2) expression was found almost completely to resemble that of HBsAg on both light and electron microscopy. Membranous pre-S(2) was seen in the liver of 16 of 25 cases who were positive for intrahepatic HBcAg. In detailed studies of membranous pre-S(2) and HBcAg, both membranous pre-S(2) and HBcAg appeared in the liver of all 7 cases before or at the peak of acute exacerbation, but after it, membranous pre-S(2) was not found in 4 of 8 cases. These results suggest that disappearance of membranous pre-S(2) and HBsAg may be related to hepatic cell necrosis in some cases with acute exacerbation.

Citing Articles

Intrahepatic expression of pre-S1 and pre-S2 antigens in chronic hepatitis B virus infection in relation to hepatitis B virus replication and hepatitis delta virus superinfection.

Chu C, Liaw Y Gut. 1992; 33(11):1544-8.

PMID: 1452081 PMC: 1379543. DOI: 10.1136/gut.33.11.1544.

References

Milich D, Thornton G, Neurath A, Kent S, Michel M, Tiollais P . Enhanced immunogenicity of the pre-S region of hepatitis B surface antigen. Science. 1985; 228(4704):1195-9. DOI: 10.1126/science.2408336. View

Heermann K, Goldmann U, Schwartz W, Seyffarth T, Baumgarten H, Gerlich W . Large surface proteins of hepatitis B virus containing the pre-s sequence. J Virol. 1984; 52(2):396-402. PMC: 254539. DOI: 10.1128/JVI.52.2.396-402.1984. View

Imai M, Yanase Y, Nojiri T, Miyakawa Y, Mayumi M . A receptor for polymerized human and chimpanzee albumins on hepatitis B virus particles co-occurring with HBeAg. Gastroenterology. 1979; 76(2):242-7. View

Machida A, Kishimoto S, Ohnuma H, Baba K, Ito Y, Miyamoto H . A polypeptide containing 55 amino acid residues coded by the pre-S region of hepatitis B virus deoxyribonucleic acid bears the receptor for polymerized human as well as chimpanzee albumins. Gastroenterology. 1984; 86(5 Pt 1):910-8. View

Kurai K, Iino S, Koike K, Mitamura K, Endo Y, Oka H . Serum titers of pre-S(2) antigen in patients with acute and chronic type B hepatitis: relation to serum aminotransferase activity and other hepatitis B virus markers. Hepatology. 1989; 9(2):175-9. DOI: 10.1002/hep.1840090202. View

Hadziyannis S, Raimondo G, Papaioannou C, Anastassakos C, Wong D, Sninsky J . Expression of pre-S gene-encoded proteins in liver and serum during chronic hepatitis B virus infection in comparison to other markers of active virus replication. J Hepatol. 1987; 5(3):253-9. DOI: 10.1016/s0168-8278(87)80029-6. View

Thung S, Gerber M, Kasambalides E, Gilja B, Keh W, Gerlich W . Demonstration of pre-S polypeptides of hepatitis B virus in infected livers. Hepatology. 1986; 6(6):1315-8. DOI: 10.1002/hep.1840060615. View

Yamada G, Nakane P . Hepatitis B core and surface antigens in liver tissue. Light and electron microscopic localization by the peroxidase-labeled antibody method. Lab Invest. 1977; 36(6):649-59. View

. Acute and chronic hepatitis revisited. Review by an international group. Lancet. 1977; 2(8044):914-9. View

10.

Fraiese A, Pontisso P, Cavalletto D, Fattovich G, Alberti A . Expression of preS1 and preS2 in the liver of chronic hepatitis B virus carriers. J Hepatol. 1988; 7(2):157-63. DOI: 10.1016/s0168-8278(88)80477-x. View

11.

Yamada G, Sakamoto Y, Mizuno M, Nishihara T, Kobayashi T, Takahashi T . Electron and immunoelectron microscopic study of Dane particle formation in chronic hepatitis B virus infection. Gastroenterology. 1982; 83(2):348-56. View

12.

Hess G, Gerlich H, Gerken G, Manns M, Meyer zum Buschenfelde K . Relationship of pre-S encoded antigens in liver and clinical manifestations of chronic hepatitis B infection. Liver. 1987; 7(5):245-50. DOI: 10.1111/j.1600-0676.1987.tb00352.x. View

13.

Thung S, Gerber M . HBsAg-associated albumin receptors and antialbumin antibodies in sera of patients with liver disease. Gastroenterology. 1981; 80(2):260-4. View

14.

KAPLAN P, Greenman R, Gerin J, Purcell R, ROBINSON W . DNA polymerase associated with human hepatitis B antigen. J Virol. 1973; 12(5):995-1005. PMC: 356728. DOI: 10.1128/JVI.12.5.995-1005.1973. View

15.

Theilmann L, Klinkert M, Gmelin K, Salfeld J, Schaller H, Pfaff E . Detection of pre-S1 proteins in serum and liver of HBsAg-positive patients: a new marker for hepatitis B virus infection. Hepatology. 1986; 6(2):186-90. DOI: 10.1002/hep.1840060206. View

16.

Neurath A, Kent S, Strick N . Location and chemical synthesis of a pre-S gene coded immunodominant epitope of hepatitis B virus. Science. 1984; 224(4647):392-5. DOI: 10.1126/science.6200931. View

17.

MERRIFIELD R . Solid-phase peptide synthesis. Adv Enzymol Relat Areas Mol Biol. 1969; 32:221-96. DOI: 10.1002/9780470122778.ch6. View

18.

Budkowska A, Riottot M, Dubreuil P, Lazizi Y, Brechot C, Sobczak E . Monoclonal antibody recognizing pre-S(2) epitope of hepatitis B virus: characterization of pre-S(2) epitope and anti-pre-S(2) antibody. J Med Virol. 1986; 20(2):111-25. DOI: 10.1002/jmv.1890200204. View

19.

Mondelli M, Vergani G, Alberti A, Vergani D, Portmann B, Eddleston A . Specificity of T lymphocyte cytotoxicity to autologous hepatocytes in chronic hepatitis B virus infection: evidence that T cells are directed against HBV core antigen expressed on hepatocytes. J Immunol. 1982; 129(6):2773-8. View