Interleukin-6 Induction by Tumor Necrosis Factor and Interleukin-1 in Human Fibroblasts Involves Activation of a Nuclear Factor Binding to a Kappa B-like Sequence

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1990 Jul 1

PMID 2192263

Citations 72

Authors

Y H Zhang

J X Lin

J Vilcek

Affiliations

Soon will be listed here.

Abstract

Using variable-length deletion constructs of the 5'-flanking region of the human interleukin-6 (IL-6) gene linked to the chloramphenicol acetyltransferase gene, we showed that the region from positions -109 to -50 mediated the bulk of the response to tumor necrosis factor (TNF) or interleukin-1 (IL-1), while it was less responsive to forskolin. DNA mobility shift assays and DNase I footprinting analysis identified a nuclear protein from TNF- or IL-1-treated fibroblasts that bound to a region comprising a kappa B-like element located between positions -72 and -63 on the IL-6 gene. On the basis of these and other experiments, we conclude that TNF and IL-1 apparently activate IL-6 gene expression by closely related mechanisms involving activation of a NF-kappa B-like factor, whereas the pathway of IL-6 induction by forskolin is, in part, different.

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