Interferon-induced Tetherin Restricts Vesicular Stomatitis Virus Release in Neurons

Overview

Journal DNA Cell Biol

Publisher Mary Ann Liebert

Specialties Cell Biology
Molecular Biology

Date 2011 Sep 17

PMID 21919738

Citations 18

Authors

Sreeja Sarojini

Thana Theofanis

Carol Shoshkes Reiss

Affiliations

Soon will be listed here.

Abstract

Tetherin, a recently identified interferon (IFN)-inducible, type 2 transmembrane protein, has been shown to be a cellular antiviral restriction factor that retains newly formed virions in infected cells. Thus, tetherin plays an important role in the innate cell-autonomous immune response. The aim of this study was to examine the antiviral activities of tetherin in vesicular stomatitis virus infections of murine neuronal cells. Both IFN-β and IFN-γ induce the expression of tetherin mRNA and protein. Tetherin knockdown experiments were carried out by transfection of tethrin shRNA into murine neuroblastoma cells using a vector containing the pCMV-driven tGFP gene. The efficiency of transfection was monitored through GFP expression by the transfected cells. Selected transfected cells were used for further mRNA and protein analysis, fluorescent immunocytolocalization, and viral infection to study the impact of tetherin knockdown. Our research indicates that tetherin is expressed on the outer face of the plasma membrane of murine neuroblastoma cells, its expression can be induced with both IFN-γ and IFN-β, and tetherin restricts progeny virus release up to 100-fold in mammalian neurons, thus contributing to a potent antiviral state within the host cell.

Citing Articles

Tetherin Restricts SARS-CoV-2 despite the Presence of Multiple Viral Antagonists.

Hagelauer E, Lotke R, Kmiec D, Hu D, Hohner M, Stopper S Viruses. 2023; 15(12).

PMID: 38140605 PMC: 10747847. DOI: 10.3390/v15122364.

FastMix: a versatile data integration pipeline for cell type-specific biomarker inference.

Zhang Y, Sun H, Mandava A, Aevermann B, Kollmann T, Scheuermann R Bioinformatics. 2022; 38(20):4735-4744.

PMID: 36018232 PMC: 9801972. DOI: 10.1093/bioinformatics/btac585.

Substitutions in Nef That Uncouple Tetherin and SERINC5 Antagonism Impair Simian Immunodeficiency Virus Replication in Primary Rhesus Macaque Lymphocytes.

Janaka S, Snow B, Behrens R, Evans D J Virol. 2022; 96(11):e0017622.

PMID: 35536019 PMC: 9175627. DOI: 10.1128/jvi.00176-22.

BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy.

Kong N, Shan T, Wang H, Jiao Y, Zuo Y, Li L Autophagy. 2019; 16(10):1737-1752.

PMID: 31868081 PMC: 8386623. DOI: 10.1080/15548627.2019.1707487.

Beyond Tethering the Viral Particles: Immunomodulatory Functions of Tetherin ().

Tiwari R, de la Torre J, McGavern D, Nayak D DNA Cell Biol. 2019; 38(11):1170-1177.

PMID: 31502877 PMC: 6939588. DOI: 10.1089/dna.2019.4777.

References

Versteeg G, Garcia-Sastre A . Viral tricks to grid-lock the type I interferon system. Curr Opin Microbiol. 2010; 13(4):508-16. PMC: 2920345. DOI: 10.1016/j.mib.2010.05.009. View

Kaletsky R, Francica J, Agrawal-Gamse C, Bates P . Tetherin-mediated restriction of filovirus budding is antagonized by the Ebola glycoprotein. Proc Natl Acad Sci U S A. 2009; 106(8):2886-91. PMC: 2650360. DOI: 10.1073/pnas.0811014106. View

Chesler D, McCutcheon J, Reiss C . Posttranscriptional regulation of neuronal nitric oxide synthase expression by IFN-gamma. J Interferon Cytokine Res. 2004; 24(2):141-9. DOI: 10.1089/107999004322813381. View

Evans D, Serra-Moreno R, Singh R, Guatelli J . BST-2/tetherin: a new component of the innate immune response to enveloped viruses. Trends Microbiol. 2010; 18(9):388-96. PMC: 2956607. DOI: 10.1016/j.tim.2010.06.010. View

Schubert D, Humphreys S, Baroni C, Cohn M . In vitro differentiation of a mouse neuroblastoma. Proc Natl Acad Sci U S A. 1969; 64(1):316-23. PMC: 286164. DOI: 10.1073/pnas.64.1.316. View

Takeuchi O, Akira S . MDA5/RIG-I and virus recognition. Curr Opin Immunol. 2008; 20(1):17-22. DOI: 10.1016/j.coi.2008.01.002. View

Sadler A, Williams B . Interferon-inducible antiviral effectors. Nat Rev Immunol. 2008; 8(7):559-68. PMC: 2522268. DOI: 10.1038/nri2314. View

Van Damme N, Goff D, Katsura C, Jorgenson R, Mitchell R, Johnson M . The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein. Cell Host Microbe. 2008; 3(4):245-52. PMC: 2474773. DOI: 10.1016/j.chom.2008.03.001. View

Trottier Jr M, Palian B, Reiss C . VSV replication in neurons is inhibited by type I IFN at multiple stages of infection. Virology. 2005; 333(2):215-25. DOI: 10.1016/j.virol.2005.01.009. View

10.

Breakfield X, Neale E, Neale J, Jacobowitz D . Localized catecholamine storage associated with granules in murine neuroblastoma cells. Brain Res. 1975; 92(2):237-56. DOI: 10.1016/0006-8993(75)90273-5. View

11.

Jolly C, Booth N, Neil S . Cell-cell spread of human immunodeficiency virus type 1 overcomes tetherin/BST-2-mediated restriction in T cells. J Virol. 2010; 84(23):12185-99. PMC: 2976402. DOI: 10.1128/JVI.01447-10. View

12.

Hinz A, Miguet N, Natrajan G, Usami Y, Yamanaka H, Renesto P . Structural basis of HIV-1 tethering to membranes by the BST-2/tetherin ectodomain. Cell Host Microbe. 2010; 7(4):314-323. PMC: 2859121. DOI: 10.1016/j.chom.2010.03.005. View

13.

Jia B, Serra-Moreno R, Neidermyer W, Rahmberg A, Mackey J, Fofana I . Species-specific activity of SIV Nef and HIV-1 Vpu in overcoming restriction by tetherin/BST2. PLoS Pathog. 2009; 5(5):e1000429. PMC: 2673686. DOI: 10.1371/journal.ppat.1000429. View

14.

van Pesch V, Lanaya H, Renauld J, Michiels T . Characterization of the murine alpha interferon gene family. J Virol. 2004; 78(15):8219-28. PMC: 446145. DOI: 10.1128/JVI.78.15.8219-8228.2004. View

15.

Dietrich I, McMonagle E, Petit S, Vijayakrishnan S, Logan N, Chan C . Feline tetherin efficiently restricts release of feline immunodeficiency virus but not spreading of infection. J Virol. 2011; 85(12):5840-52. PMC: 3126296. DOI: 10.1128/JVI.00071-11. View

16.

Rong L, Zhang J, Lu J, Pan Q, Lorgeoux R, Aloysius C . The transmembrane domain of BST-2 determines its sensitivity to down-modulation by human immunodeficiency virus type 1 Vpu. J Virol. 2009; 83(15):7536-46. PMC: 2708638. DOI: 10.1128/JVI.00620-09. View

17.

Kawai S, Azuma Y, Fujii E, Furugaki K, Ozaki S, Matsumoto T . Interferon-alpha enhances CD317 expression and the antitumor activity of anti-CD317 monoclonal antibody in renal cell carcinoma xenograft models. Cancer Sci. 2008; 99(12):2461-6. PMC: 11160063. DOI: 10.1111/j.1349-7006.2008.00968.x. View

18.

Durbin J, Hackenmiller R, Simon M, Levy D . Targeted disruption of the mouse Stat1 gene results in compromised innate immunity to viral disease. Cell. 1996; 84(3):443-50. DOI: 10.1016/s0092-8674(00)81289-1. View

19.

Vidal-Laliena M, Romero X, March S, Requena V, Petriz J, Engel P . Characterization of antibodies submitted to the B cell section of the 8th Human Leukocyte Differentiation Antigens Workshop by flow cytometry and immunohistochemistry. Cell Immunol. 2005; 236(1-2):6-16. DOI: 10.1016/j.cellimm.2005.08.002. View

20.

Samuel C . Antiviral actions of interferons. Clin Microbiol Rev. 2001; 14(4):778-809, table of contents. PMC: 89003. DOI: 10.1128/CMR.14.4.778-809.2001. View