Preparation and Antimicrobial Activity of Poly (vinyl Chloride)/gelatin/montmorillonite Biocomposite Films

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2011 Sep 13

PMID 21909641

Citations 3

Authors

Ahmed A Haroun

Eman F Ahmed

M A Abd El-Ghaffar

Affiliations

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Abstract

The aim of this study was using a novel antimicrobial thermoplastic plasticizer based on aliphatic anhydride derivative dodecenyl succinic anhydride (DSA) for blending poly (vinyl chloride), PVC, with gelatin in presence of montmorillonite (MMT) using Brabender via polymer melting technique. This anhydride-based plasticizer blended the membrane ingredients homogenously under melting process. The used plasticizer exhibited high performance antimicrobial potency for some biomedical and industrial applications. The prepared biocomposite films were evaluated for antimicrobial activity using agar disc diffusion method against gram-positive and gram-negative bacteria such as: Staphylococcus aureus (S. aureus), Klebsiella pneumonia (K. pneumonia), Bacillus cereus (B. cereus), Bacillus subtilis (B. subtilis) and Escherichia coli (E. coli). The majority of these biocomposites, except the plasticized PVC with DOP, have shown inhibitory effect at different concentrations (1.0-20) mg/ml against all above mentioned bacteria. However, C. albicans and A. niger were the most resistant strains.

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References

Weerkamp A, Uyen H, Busscher H . Effect of zeta potential and surface energy on bacterial adhesion to uncoated and saliva-coated human enamel and dentin. J Dent Res. 1988; 67(12):1483-7. DOI: 10.1177/00220345880670120801. View

Jones D, McGovern J, Woolfson A, Gorman S . Role of physiological conditions in the oropharynx on the adherence of respiratory bacterial isolates to endotracheal tube poly(vinyl chloride). Biomaterials. 1997; 18(6):503-10. DOI: 10.1016/s0142-9612(96)00170-6. View

Haroun A, Migonney V . Synthesis and in vitro evaluation of gelatin/hydroxyapatite graft copolymers to form bionanocomposites. Int J Biol Macromol. 2010; 46(3):310-6. DOI: 10.1016/j.ijbiomac.2010.01.005. View

Pena C, de la Caba K, Eceiza A, Ruseckaite R, Mondragon I . Enhancing water repellence and mechanical properties of gelatin films by tannin addition. Bioresour Technol. 2010; 101(17):6836-42. DOI: 10.1016/j.biortech.2010.03.112. View

Jones D, McGovern J, Adair C, Woolfson A, Gorman S . Conditioning film and environmental effects on the adherence of Candida spp. to silicone and poly(vinylchloride) biomaterials. J Mater Sci Mater Med. 2004; 12(5):399-405. DOI: 10.1023/a:1011240818338. View

Gomez-Estaca J, Lopez de Lacey A, Lopez-Caballero M, Gomez-Guillen M, Montero P . Biodegradable gelatin-chitosan films incorporated with essential oils as antimicrobial agents for fish preservation. Food Microbiol. 2010; 27(7):889-96. DOI: 10.1016/j.fm.2010.05.012. View

Gorman S, Mawhinney W, Adair C, Issouckis M . Confocal laser scanning microscopy of peritoneal catheter surfaces. J Med Microbiol. 1993; 38(6):411-7. DOI: 10.1099/00222615-38-6-411. View

Herrera P, Burghardt R, Phillips T . Adsorption of Salmonella enteritidis by cetylpyridinium-exchanged montmorillonite clays. Vet Microbiol. 2000; 74(3):259-72. DOI: 10.1016/s0378-1135(00)00157-7. View

Lopez P, Sanchez C, Batlle R, Nerin C . Development of flexible antimicrobial films using essential oils as active agents. J Agric Food Chem. 2007; 55(21):8814-24. DOI: 10.1021/jf071737b. View

10.

Haroun A, Gamal-Eldeen A, Harding D . Preparation, characterization and in vitro biological study of biomimetic three-dimensional gelatin-montmorillonite/cellulose scaffold for tissue engineering. J Mater Sci Mater Med. 2009; 20(12):2527-40. DOI: 10.1007/s10856-009-3818-x. View

11.

Woo G, Yang M, Yin H, Jaffer F, Mittelman M, Santerre J . Biological characterization of a novel biodegradable antimicrobial polymer synthesized with fluoroquinolones. J Biomed Mater Res. 2001; 59(1):35-45. DOI: 10.1002/jbm.1214. View

12.

Andrews J . Determination of minimum inhibitory concentrations. J Antimicrob Chemother. 2001; 48 Suppl 1:5-16. DOI: 10.1093/jac/48.suppl_1.5. View

13.

Cosentino S, Tuberoso C, Pisano B, Satta M, Mascia V, Arzedi E . In-vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Lett Appl Microbiol. 1999; 29(2):130-5. DOI: 10.1046/j.1472-765x.1999.00605.x. View