Circulating Clusterin (apolipoprotein J) Levels Do Not Have Any Day/night Variability and Are Positively Associated with Total and LDL Cholesterol Levels in Young Healthy Individuals

Overview

Journal J Clin Endocrinol Metab

Publisher Oxford University Press

Specialty Endocrinology

Date 2011 Sep 9

PMID 21900379

Citations 8

Authors

Konstantinos N Aronis

Maria T Vamvini

John P Chamberland

Christos S Mantzoros

Affiliations

Soon will be listed here.

Abstract

Context: Clusterin has been associated with several pathologies, including cardiovascular disease and neoplasias. However, little is known about its physiology and its association with metabolic and anthropometric parameters in humans.

Objective: The aim of the study was to examine whether circulating clusterin levels exhibit a day/night variation pattern and whether clusterin is associated with anthropometric and metabolic parameters.

Design: Study A was a frequent sampling study to evaluate potential periodicity in clusterin secretion. Study B was an observational study to evaluate the cross-sectional and prospective associations of clusterin with anthropometric and metabolic parameters.

Participants: Study A participants were healthy males (n = 6) and females (n = 6), aged 22.3 ± 3.1 and 22.8 ± 3.4 yr, respectively. Study B participants were 186 healthy males aged 18.4 ± 0.14 yr. Ninety-one of the study B subjects were studied again 2 yr later and clusterin's associations with change of anthropometric and metabolic parameters were thus investigated prospectively.

Intervention: Samples in study A were collected every 15 min during an overnight admission, and subsequently pooled every hour. Samples in study B were collected during a screening visit.

Main Outcome Measure: Circulating clusterin levels were measured.

Results: In study A, spectral domain and cosinor regression analysis failed to reveal any day/night variation pattern. In study B, clusterin was positively correlated with total and low-density lipoprotein cholesterol (r = 0.23, P = 0.002; and r = 0.20, P = 0.005). Baseline clusterin did not predict change of any anthropometric, biochemical, or metabolic parameters prospectively.

Conclusions: We report for the first time that circulating clusterin does not have a day/night variation pattern in healthy young individuals. Clusterin levels are associated with total and low-density lipoprotein cholesterol cross-sectionally but do not predict short-term changes in metabolic parameters in healthy young males.

Citing Articles

Monocyte Chemoattractant Protein-1 (MCP-1), Activin-A and Clusterin in Children and Adolescents with Obesity or Type-1 Diabetes Mellitus.

Kostopoulou E, Kalavrizioti D, Davoulou P, Papachristou E, Sinopidis X, Fouzas S Diagnostics (Basel). 2024; 14(4).

PMID: 38396489 PMC: 10887959. DOI: 10.3390/diagnostics14040450.

Sterol O-acyltransferase 2 chaperoned by apolipoprotein J facilitates hepatic lipid accumulation following viral and nutrient stresses.

Sun H, Chen T, Tan Y, Wang C, Young K Commun Biol. 2021; 4(1):564.

PMID: 33980978 PMC: 8115332. DOI: 10.1038/s42003-021-02093-2.

Circulating IL-6, clusterin and irisin in obese subjects with different grades of obesity: association with insulin resistance and sexual dimorphism.

Werida R, El-Gharbawy N, Mostafa T Arch Endocrinol Metab. 2021; 65(2):126-136.

PMID: 33905632 PMC: 10065324. DOI: 10.20945/2359-3997000000336.

Clusterin and Its Role in Insulin Resistance and the Cardiometabolic Syndrome.

Wittwer J, Bradley D Front Immunol. 2021; 12:612496.

PMID: 33717095 PMC: 7946829. DOI: 10.3389/fimmu.2021.612496.

Combined Aerobic and Resistance Exercise Training Reduces Circulating Apolipoprotein J Levels and Improves Insulin Resistance in Postmenopausal Diabetic Women.

Jeon Y, Kim S, Kim J, Kim H, Kim H, Park J Diabetes Metab J. 2020; 44(1):103-112.

PMID: 32097999 PMC: 7043986. DOI: 10.4093/dmj.2018.0160.

References

Bettuzzi S . Conclusions and perspectives. Adv Cancer Res. 2009; 105:133-50. DOI: 10.1016/S0065-230X(09)05008-8. View

Blaschuk O, BURDZY K, Fritz I . Purification and characterization of a cell-aggregating factor (clusterin), the major glycoprotein in ram rete testis fluid. J Biol Chem. 1983; 258(12):7714-20. View

Trougakos I, Poulakou M, Stathatos M, Chalikia A, Melidonis A, Gonos E . Serum levels of the senescence biomarker clusterin/apolipoprotein J increase significantly in diabetes type II and during development of coronary heart disease or at myocardial infarction. Exp Gerontol. 2002; 37(10-11):1175-87. DOI: 10.1016/s0531-5565(02)00139-0. View

Hamnvik O, Liu X, Petrou M, Gong H, Chamberland J, Kim E . Soluble leptin receptor and leptin are associated with baseline adiposity and metabolic risk factors, and predict adiposity, metabolic syndrome, and glucose levels at 2-year follow-up: the Cyprus Metabolism Prospective Cohort Study. Metabolism. 2010; 60(7):987-93. DOI: 10.1016/j.metabol.2010.09.009. View

de Silva H, Stuart W, Duvic C, Wetterau J, Ray M, Ferguson D . A 70-kDa apolipoprotein designated ApoJ is a marker for subclasses of human plasma high density lipoproteins. J Biol Chem. 1990; 265(22):13240-7. View

Kujiraoka T, Hattori H, Miwa Y, Ishihara M, Ueno T, Ishii J . Serum apolipoprotein j in health, coronary heart disease and type 2 diabetes mellitus. J Atheroscler Thromb. 2006; 13(6):314-22. DOI: 10.5551/jat.13.314. View

Daimon M, Oizumi T, Karasawa S, Kaino W, Takase K, Tada K . Association of the clusterin gene polymorphisms with type 2 diabetes mellitus. Metabolism. 2010; 60(6):815-22. DOI: 10.1016/j.metabol.2010.07.033. View

Mantzoros C, Liolios A, Tritos N, Kaklamani V, Doulgerakis D, Griveas I . Circulating insulin concentrations, smoking, and alcohol intake are important independent predictors of leptin in young healthy men. Obes Res. 1998; 6(3):179-86. DOI: 10.1002/j.1550-8528.1998.tb00335.x. View

Mantzoros C, Trakatelli M, Gogas H, Dessypris N, Stratigos A, Chrousos G . Circulating adiponectin levels in relation to melanoma: a case-control study. Eur J Cancer. 2007; 43(9):1430-6. DOI: 10.1016/j.ejca.2007.03.026. View

10.

Bluher M, Brennan A, Kelesidis T, Kratzsch J, Fasshauer M, Kralisch S . Total and high-molecular weight adiponectin in relation to metabolic variables at baseline and in response to an exercise treatment program: comparative evaluation of three assays. Diabetes Care. 2007; 30(2):280-5. DOI: 10.2337/dc06-1362. View

11.

Panico F, Rizzi F, Fabbri L, Bettuzzi S, Luppi F . Clusterin (CLU) and lung cancer. Adv Cancer Res. 2009; 105:63-76. DOI: 10.1016/S0065-230X(09)05004-0. View

12.

Kelesidis T, Kelesidis I, Chou S, Mantzoros C . Narrative review: the role of leptin in human physiology: emerging clinical applications. Ann Intern Med. 2010; 152(2):93-100. PMC: 2829242. DOI: 10.7326/0003-4819-152-2-201001190-00008. View

13.

Chan J, Matarese G, Shetty G, Raciti P, Kelesidis I, Aufiero D . Differential regulation of metabolic, neuroendocrine, and immune function by leptin in humans. Proc Natl Acad Sci U S A. 2006; 103(22):8481-6. PMC: 1482518. DOI: 10.1073/pnas.0505429103. View

14.

Chan J, Heist K, DePaoli A, Veldhuis J, Mantzoros C . The role of falling leptin levels in the neuroendocrine and metabolic adaptation to short-term starvation in healthy men. J Clin Invest. 2003; 111(9):1409-21. PMC: 154448. DOI: 10.1172/JCI17490. View

15.

de Silva H, Stuart W, Park Y, Mao S, Gil C, Wetterau J . Purification and characterization of apolipoprotein J. J Biol Chem. 1990; 265(24):14292-7. View

16.

Hoofnagle A, Wu M, Gosmanova A, Becker J, Wijsman E, Brunzell J . Low clusterin levels in high-density lipoprotein associate with insulin resistance, obesity, and dyslipoproteinemia. Arterioscler Thromb Vasc Biol. 2010; 30(12):2528-34. PMC: 2988100. DOI: 10.1161/ATVBAHA.110.212894. View

17.

Aronis K, Kim Y, Mantzoros C . Clusterin (apolipoprotein J): wither link with diabetes and cardiometabolic risk?. Metabolism. 2011; 60(6):747-8. DOI: 10.1016/j.metabol.2010.12.013. View

18.

Wolfe B, Jimerson D, Orlova C, Mantzoros C . Effect of dieting on plasma leptin, soluble leptin receptor, adiponectin and resistin levels in healthy volunteers. Clin Endocrinol (Oxf). 2004; 61(3):332-8. DOI: 10.1111/j.1365-2265.2004.02101.x. View

19.

Rizzi F, Bettuzzi S . Clusterin (CLU) and prostate cancer. Adv Cancer Res. 2009; 105:1-19. DOI: 10.1016/S0065-230X(09)05001-5. View

20.

Mantzoros C . Role of leptin in reproduction. Ann N Y Acad Sci. 2000; 900:174-83. DOI: 10.1111/j.1749-6632.2000.tb06228.x. View