Studies on Human CFU-Mix Microvolume Culture by Use of Limiting Dilution Assay

Overview

Journal J Tongji Med Univ

Specialty General Medicine

Date 1990 Jan 1

PMID 2190000

Authors

Y Chen

B M Wang

C Y Li

D I Yu

Affiliations

Soon will be listed here.

Abstract

By use of limiting dilution assay we investigated the bipotent and pluripotent hemopoietic progenitor cells (CFU-Mix) from 21 normal adults, cultured for 32 times. The CFU-Mix counts were 35.7 colonies/10(6) bone marrow cells. According to different cellular elements they could be divided into 5 groups, namely GEMM, GE, GL, GMeg and GM phi. It was suggested that differentiation of the stem cell is of stochastic process, influenced by different hemopoietic growth factors and cellular microenvironment. Our model seems to be suitable for studying differentiation of the stem cells. The rates of 3H-TdR and 55 + 59Fe incorporation were determined, and the 2nd day of culture was found to be the delayed stage of cell growth.

References

Messner H, Fauser A . Culture studies of human pluripotent hemopoietic progenitors. Blut. 1980; 41(5):327-33. DOI: 10.1007/BF01035488. View

Udupa K, Crabtree H, Lipschitz D . In vitro culture of proerythroblasts: characterization of proliferative response to erythropoietin and steroids. Br J Haematol. 1986; 62(4):705-14. DOI: 10.1111/j.1365-2141.1986.tb04094.x. View

Konwalinka G, Peschel C, Boyd J, Geissler D, Ogriseg M, ODAVIC R . A miniaturized agar culture system for cloning human erythropoietic progenitor cells. Exp Hematol. 1984; 12(1):75-9. View

Nakahata T, Tsuji K, Ishiguro A, ANDO O, Norose N, Koike K . Single-cell origin of human mixed hemopoietic colonies expressing various combinations of cell lineages. Blood. 1985; 65(4):1010-6. View

Ash R, Detrick D, Zanjani E . Studies of human pluripotential hemopoietic stem cells (CFU-GEMM) in vitro. Blood. 1981; 58(2):309-16. View