PCR Could Be a Method of Choice for Identification of Both Pulmonary and Extra-pulmonary Tuberculosis

Overview

Journal BMC Res Notes

Publisher Biomed Central

Specialties Biology
General Medicine

Date 2011 Sep 9

PMID 21899772

Citations 13

Authors

Iram Amin

Muhammad Idrees

Zunaira Awan

Muhammad Shahid

Samia Afzal

Abrar Hussain

Affiliations

Soon will be listed here.

Abstract

Background: Nucleic acid amplification assays including PCR have revolutionized the detection of Mycobacterium tuberculosis (MTB). Tuberculosis spread to almost every organ of the body and is characterized on the basis of localization of infection. Therefore, different types of body fluids and tissues can be used for the detection of MTB.From 2004 to 2010 total 766 different types of smear negative samples from patients, clinically suspected for tuberculosis were received and investigated at Division of Molecular Diagnostics, University of the Punjab Lahore for the diagnosis of tuberculosis. Mycobacterial DNA was extracted followed by PCR amplification.

Findings: A total of 356 (46.5%) samples were found positive by PCR for MTB. These included; serum (4.8%), blood (36.3%), urine (46.6%), cerebro spinal fluid (CSF) (42.1%), ascetic fluid (67.6%), pleural fluid (52%), pericardial fluid (30%), pus (38.6%), bone marrow (60%), sputum (38.8%) and bronchoalveolar lavage (BAL) (70%). Over all there was no significant difference in males and females neither in different age groups for the identification of MTB.

Conclusion: We conclude that PCR is a useful and sensitive tool for the early diagnosis of MTB in variety of clinical samples.

Citing Articles

Natural outbreak of infection in imported laboratory cynomolgus macaques (): diagnostic pitfalls and management of safety precautions.

Weber K, Mayoral F, Vallejo C, Sanchez R, Hartelust R, Mendoza P J Toxicol Pathol. 2024; 37(4):197-206.

PMID: 39359895 PMC: 11442260. DOI: 10.1293/tox.2024-0048.

Hard Ticks as Vectors: The Emerging Threat of Tick-Borne Diseases in India.

Perumalsamy N, Sharma R, Subramanian M, Nagarajan S Pathogens. 2024; 13(7).

PMID: 39057783 PMC: 11279560. DOI: 10.3390/pathogens13070556.

Diagnosis and Treatment of Skipped Multifocal Spinal Tuberculosis Lesions.

Na S, Lyu Z, Zhang S Orthop Surg. 2023; 15(6):1454-1467.

PMID: 37186216 PMC: 10235186. DOI: 10.1111/os.13744.

Comparative Performance of Line Probe Assay and GeneXpert in the Detection of Rifampicin Monoresistance in a TB-Endemic African Country.

Mchaki B, Mgaya F, Kunambi P, Hangombe B, Matee M, Munyeme M Antibiotics (Basel). 2022; 11(11).

PMID: 36358145 PMC: 9686643. DOI: 10.3390/antibiotics11111489.

Pulmonary tuberculosis presenting as lung abscess in a 10-month-Old infant.

Thadchanamoorthy V, Dayasiri K Respir Med Case Rep. 2020; 31:101229.

PMID: 32995265 PMC: 7516287. DOI: 10.1016/j.rmcr.2020.101229.

References

Shah S, Miller A, Mastellone A, Kim K, Colaninno P, Hochstein L . Rapid diagnosis of tuberculosis in various biopsy and body fluid specimens by the AMPLICOR Mycobacterium tuberculosis polymerase chain reaction test. Chest. 1998; 113(5):1190-4. DOI: 10.1378/chest.113.5.1190. View

Perera J, Chandrasekharan N, Karunanayake E . PCR based detection of Mycobacterium tuberculosis: effect of sample preparation. Southeast Asian J Trop Med Public Health. 1994; 25(4):693-7. View

Jabeen K, Shakoor S, Chishti S, Ayaz A, Hasan R . Fluoroquinolone-resistant Mycobacterium tuberculosis, Pakistan, 2005-2009. Emerg Infect Dis. 2011; 17(3):564-6. PMC: 3166006. DOI: 10.3201/eid1703.100957. View

Hasan R, Jabeen K, Mehraj V, Zafar F, Malik F, Hassan Q . Trends in Mycobacterium tuberculosis resistance, Pakistan, 1990-2007. Int J Infect Dis. 2009; 13(6):e377-82. DOI: 10.1016/j.ijid.2009.01.008. View

Thornton C, MacLellan K, Brink Jr T, Lockwood D, Romagnoli M, Turner J . Novel method for processing respiratory specimens for detection of mycobacteria by using C18-carboxypropylbetaine: blinded study. J Clin Microbiol. 1998; 36(7):1996-2003. PMC: 104967. DOI: 10.1128/JCM.36.7.1996-2003.1998. View

Singh K, Muralidhar M, Kumar A, Chattopadhyaya T, Kapila K, Singh M . Comparison of in house polymerase chain reaction with conventional techniques for the detection of Mycobacterium tuberculosis DNA in granulomatous lymphadenopathy. J Clin Pathol. 2000; 53(5):355-61. PMC: 1731191. DOI: 10.1136/jcp.53.5.355. View

Sarmiento O, Weigle K, Alexander J, Weber D, Miller W . Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis. J Clin Microbiol. 2003; 41(7):3233-40. PMC: 165327. DOI: 10.1128/JCM.41.7.3233-3240.2003. View

Kwok S, Higuchi R . Avoiding false positives with PCR. Nature. 1989; 339(6221):237-8. DOI: 10.1038/339237a0. View

Manganelli R, DUBNAU E, Tyagi S, Kramer F, Smith I . Differential expression of 10 sigma factor genes in Mycobacterium tuberculosis. Mol Microbiol. 1999; 31(2):715-24. DOI: 10.1046/j.1365-2958.1999.01212.x. View

10.

Soini H, Musser J . Molecular diagnosis of mycobacteria. Clin Chem. 2001; 47(5):809-14. View

11.

Eisenach K, Cave M, Bates J, Crawford J . Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. J Infect Dis. 1990; 161(5):977-81. DOI: 10.1093/infdis/161.5.977. View

12.

Hale Y, Pfyffer G, Salfinger M . Laboratory diagnosis of mycobacterial infections: new tools and lessons learned. Clin Infect Dis. 2001; 33(6):834-46. DOI: 10.1086/322607. View

13.

Styrt B, Shinnick T, Ridderhof J, Crawford J, Tenover F . Turnaround times for mycobacterial cultures. J Clin Microbiol. 1997; 35(4):1041-2. PMC: 229736. DOI: 10.1128/jcm.35.4.1041-1042.1997. View

14.

Farnia P, Mohammadi F, Zarifi Z, Tabatabee D, Ganavi J, Ghazisaeedi K . Improving sensitivity of direct microscopy for detection of acid-fast bacilli in sputum: use of chitin in mucus digestion. J Clin Microbiol. 2002; 40(2):508-11. PMC: 153416. DOI: 10.1128/JCM.40.2.508-511.2002. View

15.

Selvakumar N, Rahman F, Garg R, Rajasekaran S, Mohan N, Thyagarajan K . Evaluation of the phenol ammonium sulfate sedimentation smear microscopy method for diagnosis of pulmonary tuberculosis. J Clin Microbiol. 2002; 40(8):3017-20. PMC: 120642. DOI: 10.1128/JCM.40.8.3017-3020.2002. View

16.

Greco S, Rulli M, Girardi E, Piersimoni C, Saltini C . Diagnostic accuracy of in-house PCR for pulmonary tuberculosis in smear-positive patients: meta-analysis and metaregression. J Clin Microbiol. 2009; 47(3):569-76. PMC: 2650956. DOI: 10.1128/JCM.02051-08. View

17.

Telenti A, Marchesi F, Balz M, Bally F, Bottger E, Bodmer T . Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol. 1993; 31(2):175-8. PMC: 262730. DOI: 10.1128/jcm.31.2.175-178.1993. View

18.

Coninx R . Tuberculosis in complex emergencies. Bull World Health Organ. 2007; 85(8):637-40. PMC: 2636383. DOI: 10.2471/blt.06.037630. View