Genome Instability in AZFc Region on Y Chromosome in Leukocytes of Fertile and Infertile Individuals Following Exposure to Gamma Radiation

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2011 Sep 8

PMID 21898105

Citations 4

Authors

Sahar Moghbeli-Nejad

Hossein Mozdarani

Mehrdad Behmanesh

Zahra Rezaiean

Parvin Fallahi

Affiliations

Soon will be listed here.

Abstract

Purpose: Men are exposed to various doses of ionizing radiation due to living in regions with high natural background radiation, accidentally, occupationally or for cancer treatment. To study genomic instability of AZFc region to gamma radiation, blood samples from normal, oligozoospermia, and azoospermia individuals were irradiated by a Co-60 source.

Methods: Irradiated cells were kept for 48 h in order to repair initial DNA damages. Real time PCR was performed for three markers (SY 1206, SY 1197, SY 579) for testing copy number variation before and after irradiation. Copy number variations were compared by calculation of cycle threshold comparative method.

Results: Copy number variations of studied markers in AZFc region (microdeletion and duplication) in all samples after exposure to radiation increased with a dose dependent fashion. The frequency of instability was significantly higher in samples from infertile men in comparison with fertile ones (p < 0.001).

Conclusion: No significant difference was seen between the two infertile groups (p > 0.05). This observation might be a possible explanation for induction of azoospermia and oligozoospermia after radiotherapy. Increased frequency of induced microdeletion and duplication in infertile men compared with normal might be attributed to the deficiency in repair systems and the genetic factors involved in incomplete spermatogenesis of infertile men.

Citing Articles

High frequency of microdeletion in gene family in peripheral blood leukocytes of non-obstructive azoospermia patients.

Zonozi F, Mozdarani H, Salimi M, Mozdarani S, Fallahi P, Mozdarani S AIMS Genet. 2019; 4(4):202-212.

PMID: 31435509 PMC: 6690235. DOI: 10.3934/genet.2017.4.202.

Basic and clinical genetic studies on male infertility in Iran during 2000-2016: A review.

Moghbelinejad S, Mozdarani H, Ghoraeian P, Asadi R Int J Reprod Biomed. 2018; 16(3):131-148.

PMID: 29766145 PMC: 5944436.

Incidence of Y-chromosome microdeletions in children whose fathers underwent vasectomy reversal or in vitro fertilization with epididymal sperm aspiration: a case-control study.

Ghirelli-Filho M, Marchi P, Mafra F, Cavalcanti V, Christofolini D, Barbosa C Einstein (Sao Paulo). 2017; 14(4):534-540.

PMID: 28076602 PMC: 5221381. DOI: 10.1590/S1679-45082016AO3805.

Efficient combined FISH and PRINS technique for detection of DAZ microdeletion in human sperm.

Mozdarani H, Ghoraeian P J Assist Reprod Genet. 2012; 29(9):979-84.

PMID: 22648284 PMC: 3463660. DOI: 10.1007/s10815-012-9805-z.

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