Tubular Immunostimulating Complex Based on Cucumarioside A2-2 and Monogalactosyldiacylglycerol from Marine Macrophytes

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2011 Sep 6

PMID 21888630

Citations 7

Authors

Eduard Y Kostetsky

Nina M Sanina

Andrey N Mazeika

Alexander V Tsybulsky

Natalia S Vorobyeva

Valery L Shnyrov

Affiliations

Soon will be listed here.

Abstract

Background: There is an urgent need to develop safe and effective adjuvants for the new generation of subunit vaccines. We developed the tubular immunostimulating complex (TI-complex) as a new nanoparticulate antigen delivery system. The morphology and composition of TI-complexes principally differ from the known vesicular immunostimulating complexes (ISCOMs). However, methodology for the preparation of TI-complexes has suffered a number of shortcomings. The aim of the present work was to obtain an antigen carrier consisting of triterpene glycosides from Cucumaria japonica, cholesterol, and monogalactosyldiacylglycerol from marine macrophytes with reproducible properties and high adjuvant activity.

Results: The cucumarioside A2-2 - cholesterol - MGalDG ratio of 6:2:4 (by weight) was found to provide the most effective formation of TI-complexes and the minimum hemolytic activity in vitro. Tubules of TI-complexes have an outer diameter of about 16 nm, an inner diameter of 6 nm, and a length of 500 nm. A significant dilution by the buffer gradually destroyed the tubular nanoparticles. The TI-complex was able to increase the immunogenicity of the protein antigens from Yersinia pseudotuberculosis by three to four times.

Conclusions: We propose an optimized methodology for the preparation of homogeneous TI-complexes containing only tubular particles, which would achieve reproducible immunization results. We suggest that the elaborated TI-complexes apply as a universal delivery system for different subunit antigens within anti-infectious vaccines and enhance their economic efficacy and safety.

Citing Articles

Vaccine Adjuvants Derived from Marine Organisms.

Sanina N Biomolecules. 2019; 9(8).

PMID: 31382606 PMC: 6723903. DOI: 10.3390/biom9080340.

Fatty Acid Composition and Thermotropic Behavior of Glycolipids and Other Membrane Lipids of Ulva lactuca (Chlorophyta) Inhabiting Different Climatic Zones.

Kostetsky E, Chopenko N, Barkina M, Velansky P, Sanina N Mar Drugs. 2018; 16(12).

PMID: 30544629 PMC: 6316251. DOI: 10.3390/md16120494.

Immunogenicity and Protective Activity of a Chimeric Protein Based on the Domain III of the Tick-Borne Encephalitis Virus E Protein and the OmpF Porin of Incorporated into the TI-Complex.

Sanina N, Chopenko N, Mazeika A, Davydova L, Leonova G, Stenkova A Int J Mol Sci. 2018; 19(10).

PMID: 30274357 PMC: 6213927. DOI: 10.3390/ijms19102988.

Recombinant Fusion Protein Joining E Protein Domain III of Tick-Borne Encephalitis Virus and HSP70 of as an Antigen for the TI-Complexes.

Golotin V, Sanina N, Davydova L, Chopenko N, Mazeika A, Roig M Biomolecules. 2018; 8(3).

PMID: 30149603 PMC: 6164642. DOI: 10.3390/biom8030082.

Modulation of Immunogenicity and Conformation of HA1 Subunit of Influenza A Virus H1/N1 Hemagglutinin in Tubular Immunostimulating Complexes.

Sanina N, Davydova L, Chopenko N, Kostetsky E, Shnyrov V Int J Mol Sci. 2017; 18(9).

PMID: 28869526 PMC: 5618544. DOI: 10.3390/ijms18091895.

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