Identification of Enriched Conjugated Linoleic Acid Isomers in Cultures of Ruminal Microorganisms After Dosing with 1-(13)C-linoleic Acid

Overview

Journal J Microbiol

Specialty Microbiology

Date 2011 Sep 3

PMID 21887646

Citations 1

Authors

Yong-Jae Lee

Thomas C Jenkins

Affiliations

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Abstract

Most studies of linoleic acid biohydrogenation propose that it converts to stearic acid through the production of cis-9 trans-11 CLA and trans-11 C18:1. However, several other CLA have been identified in ruminai contents, suggesting additional pathways may exist. To explore this possibility, this research investigated the linoleic acid biohydrogenation pathway to identify CLA isomers in cultures of ruminai microorganisms after dosing with a (13)C stable isotope. The (13)C enrichment was calculated as [(M+1/M)×100] in labeled minus unlabeled cultures. After 48 h incubation, significant (13)C enrichment was observed in seven CLA isomers, indicating their formation from linoleic acid. All enriched CLA isomers had double bonds in either the 9,11 or 10,12 position except for trans-9 cis-11 CLA. The cis-9 trans-11 CLA exhibited the highest enrichment (30.65%), followed by enrichments from 21.06 to 23.08% for trans-10 cis-12, cis-10 trans-12, trans-9 trans-11, and trans-10 trans-12 CLA. The remaining two CLA (cis-9 cis-11 and cis-10 cis-12 CLA) exhibited enrichments of 18.38 and 19.29%, respectively. The results of this study verified the formation of cis-9 trans-11 and trans-10 cis-12 CLA isomers from linoleic acid biohydrogenation. An additional five CLA isomers also contained carbons originating from linoleic acid, indicating that pathways of linoleic acid biohydrogenation are more complex than previously described.

Citing Articles

Conditions Associated with Marine Lipid-Induced Milk Fat Depression in Sheep Cause Shifts in the In Vitro Ruminal Metabolism of 1-C Oleic Acid.

Toral P, Hervas G, Peiro V, Frutos P Animals (Basel). 2018; 8(11).

PMID: 30400269 PMC: 6262421. DOI: 10.3390/ani8110196.

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