The Essential Genome of a Bacterium

Overview

Journal Mol Syst Biol

Specialty Molecular Biology

Date 2011 Sep 1

PMID 21878915

Citations 186

Authors

Beat Christen

Eduardo Abeliuk

John M Collier

Virginia S Kalogeraki

Ben Passarelli

John A Coller

Michael J Fero

Harley H McAdams

Lucy Shapiro

Affiliations

Soon will be listed here.

Abstract

Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle. Full discovery of its essential genome, including non-coding, regulatory and coding elements, is a prerequisite for understanding the complete regulatory network of a bacterial cell. Using hyper-saturated transposon mutagenesis coupled with high-throughput sequencing, we determined the essential Caulobacter genome at 8 bp resolution, including 1012 essential genome features: 480 ORFs, 402 regulatory sequences and 130 non-coding elements, including 90 intergenic segments of unknown function. The essential transcriptional circuitry for growth on rich media includes 10 transcription factors, 2 RNA polymerase sigma factors and 1 anti-sigma factor. We identified all essential promoter elements for the cell cycle-regulated genes. The essential elements are preferentially positioned near the origin and terminus of the chromosome. The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species.

Citing Articles

An sRNA overexpression library reveals AbnZ as a negative regulator of an essential translocation module in Caulobacter crescentus.

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Essential Genes Discovery in Microorganisms by Transposon-Directed Sequencing (Tn-Seq): Experimental Approaches, Major Goals, and Future Perspectives.

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Diribonuclease activity eliminates toxic diribonucleotide accumulation.

Kim S, Orr M, Turdiev H, Jenkins C, Lormand J, Myers T Cell Rep. 2024; 43(9):114759.

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