Phylogenetic Analysis of Urease-positive Thermophilic Campylobacter (UPTC) Strains Based on the Molecular Characterization of the FlaA Gene

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2011 Aug 30

PMID 21874596

Authors

Mayumi Murayama

Akihiro Tazumi

Kyohei Hayashi

Shigeyuki Nakanishi

Erina Tasaki

Hitomi Ueno

Takuya Nakajima

Keiko Matsubara

John E Moore

Beverly C Millar

Motoo Matsuda

Affiliations

Soon will be listed here.

Abstract

Molecular cloning, nucleotide sequencing, and characterization of the flaA gene from additional isolates of urease-positive thermophilic Campylobacter (UPTC) were performed. These isolates were obtained from the natural environment in Northern Ireland (n = 9 from mussels) and in England (n = 1 from sea water). All isolates carried the shorter flaA gene, [open reading frames (ORFs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaA pseudogenes. The UPTC isolates were well discriminated by the neighbor joining (NJ) phylogenetic tree constructed based on the putative flaA genes ORFs nucleotide sequence information. In addition, the NJ tree constructed based on the flaA-short variable region sequence information discriminated the Campylobacter lari isolates with a similar degree of discrimination power.

References

Nuijten P, Van Asten F, Gaastra W, van der Zeijst B . Structural and functional analysis of two Campylobacter jejuni flagellin genes. J Biol Chem. 1990; 265(29):17798-804. View

Nachamkin I, Bohachick K, Patton C . Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J Clin Microbiol. 1993; 31(6):1531-6. PMC: 265573. DOI: 10.1128/jcm.31.6.1531-1536.1993. View

Sekizuka T, Seki K, Hayakawa T, Moore J, Murayama O, Matsuda M . Phenotypic characterisation of flagellin and flagella of urease-positive thermophilic campylobacters. Br J Biomed Sci. 2005; 61(4):186-9. DOI: 10.1080/09674845.2004.11732669. View

Sekizuka T, Gondo T, Murayama O, Moore J, Millar B, Matsuda M . flaA-like sequences containing internal termination codons (TAG) in urease-positive thermophilic Campylobacter isolated in Japan. Lett Appl Microbiol. 2002; 35(3):185-9. DOI: 10.1046/j.1472-765x.2002.01167.x. View

SKIRROW M, Benjamin J . '1001' Campylobacters: cultural characteristics of intestinal campylobacters from man and animals. J Hyg (Lond). 1980; 85(3):427-42. PMC: 2134020. DOI: 10.1017/s0022172400063506. View

Hirayama J, Tazumi A, Nakanishi S, Tasaki E, Hayashi K, Moore J . Reliability of nucleotide sequence information of full-length flagellin A gene (flaA) and flaA short variable region (SVR) for molecular discrimination of Campylobacter lari organisms. Folia Microbiol (Praha). 2011; 56(2):103-9. DOI: 10.1007/s12223-011-0026-0. View

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View

Werno A, Klena J, Shaw G, Murdoch D . Fatal case of Campylobacter lari prosthetic joint infection and bacteremia in an immunocompetent patient. J Clin Microbiol. 2002; 40(3):1053-5. PMC: 120280. DOI: 10.1128/JCM.40.3.1053-1055.2002. View

Harrington C, Thomson-Carter F, Carter P . Evidence for recombination in the flagellin locus of Campylobacter jejuni: implications for the flagellin gene typing scheme. J Clin Microbiol. 1997; 35(9):2386-92. PMC: 229973. DOI: 10.1128/jcm.35.9.2386-2392.1997. View

10.

Sekizuka T, Yokoi T, Murayama O, Millar B, Moore J, Matsuda M . A newly constructed primer pair for the PCR amplification, cloning and sequencing of the flagellin (flaA) gene from isolatesof urease-negative Campylobacter lari. Antonie Van Leeuwenhoek. 2005; 88(2):113-20. DOI: 10.1007/s10482-005-2386-4. View

11.

Sekizuka T, Gondo T, Murayama O, Kato Y, Moore J, Millar B . Molecular cloning, nucleotide sequencing and characterization of the flagellin gene from isolates of urease-positive thermophilic Campylobacter. Res Microbiol. 2004; 155(3):185-91. DOI: 10.1016/j.resmic.2003.12.003. View

12.

Meinersmann R, Helsel L, Fields P, Hiett K . Discrimination of Campylobacter jejuni isolates by fla gene sequencing. J Clin Microbiol. 1997; 35(11):2810-4. PMC: 230067. DOI: 10.1128/jcm.35.11.2810-2814.1997. View

13.

Bolton F, Holt A, Hutchinson D . Urease-positive thermophilic campylobacters. Lancet. 1985; 1(8439):1217-8. DOI: 10.1016/s0140-6736(85)92898-3. View

14.

Sekizuka T, Murayama O, Moore J, Millar B, Matsuda M . Flagellin gene structure of flaA and flaB and adjacent gene loci in urease-positive thermophilic Campylobacter (UPTC). J Basic Microbiol. 2007; 47(1):63-73. DOI: 10.1002/jobm.200610194. View

15.

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

16.

Guerry P, Logan S, Thornton S, Trust T . Genomic organization and expression of Campylobacter flagellin genes. J Bacteriol. 1990; 172(4):1853-60. PMC: 208679. DOI: 10.1128/jb.172.4.1853-1860.1990. View

17.

Gondo T, Sekizuka T, Manaka N, Murayama O, Millar B, Moore J . Demonstration of the shorter flagellin (flaA) gene of urease-positive thermophilic Campylobacter isolated from the natural environment in Northern Ireland. Folia Microbiol (Praha). 2006; 51(3):183-90. DOI: 10.1007/BF02932120. View

18.

Nachamkin I, Stowell C, Skalina D, Jones A, Hoop 2nd R, Smibert R . Campylobacter laridis causing bacteremia in an immunosuppressed patient. Ann Intern Med. 1984; 101(1):55-7. DOI: 10.7326/0003-4819-101-1-55. View

19.

Matsuda M, Moore J . Urease-positive thermophilic Campylobacter species. Appl Environ Microbiol. 2004; 70(8):4415-8. PMC: 492433. DOI: 10.1128/AEM.70.8.4415-4418.2004. View

20.

Meinersmann R, Phillips R, Hiett K, Fedorka-Cray P . Differentiation of campylobacter populations as demonstrated by flagellin short variable region sequences. Appl Environ Microbiol. 2005; 71(10):6368-74. PMC: 1265918. DOI: 10.1128/AEM.71.10.6368-6374.2005. View