Prediction of Oocyte Developmental Competence in Ovine Using Glucose-6-phosphate Dehydrogenase (G6PDH) Activity Determined at Retrieval Time

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2011 Aug 27

PMID 21870182

Citations 11

Authors

Abdollah Mohammadi-Sangcheshmeh

Masoud Soleimani

Hamid Deldar

Mohammad Salehi

Sara Soudi

Seyed Mahmoud Hashemi

Karl Schellander

Michael Hoelker

Affiliations

Soon will be listed here.

Abstract

Purpose: To determine whether G6PDH-activity measured by Brilliant Cresyl Blue known as BCB dye, predicts developmental competence within cohorts of ovine oocytes.

Methods: Ovine oocytes were exposed to BCB staining and categorized into two groups: BCB+ (blue cytoplasm, low G6PDH-activity) and BCB- (colorless cytoplasm, high G6PDH-activity). After maturation in vitro, oocytes were subjected to fertilization followed by in vitro embryo culture.

Results: We observed a significant difference in oocyte diameter considering BCB+ and BCB- oocytes. BCB+ and Control groups showed significantly higher maturation rates compared to BCB- group. There were significantly more cleaved embryos in BCB+ and control groups than in BCB- group. Blastocyst rate was significantly higher for BCB+ group compared to control and BCB- groups with control group being significantly higher than BCB- group.

Conclusion: G6PDH-activity is a strong predictive marker of oocyte competence and may be useful in identifying oocytes with a good prognosis for further develop.

Citing Articles

Oocyte Morphometric Assessment and Gene Expression Profiling of Oocytes and Cumulus Cells as Biomarkers of Oocyte Competence in Sheep.

Maside C, Sanchez-Ajofrin I, Medina-Chavez D, Alves B, Garde J, Soler A Animals (Basel). 2021; 11(10).

PMID: 34679840 PMC: 8532595. DOI: 10.3390/ani11102818.

The Function of Cumulus Cells in Oocyte Growth and Maturation and in Subsequent Ovulation and Fertilization.

Turathum B, Gao E, Chian R Cells. 2021; 10(9).

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Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats.

Veshkini A, Mohammadi-Sangcheshmeh A, Ghanem N, Abazari-Kia A, Mottaghi E, Kamaledini R Anim Reprod. 2021; 15(2):124-134.

PMID: 34122643 PMC: 8186877. DOI: 10.21451/1984-3143-2017-AR986.

Brilliant cresyl blue enhanced optoacoustic imaging enables non-destructive imaging of mammalian ovarian follicles for artificial reproduction.

Dutta R, Mandal S, Lin H, Raz T, Kind A, Schnieke A J R Soc Interface. 2020; 17(172):20200776.

PMID: 33143591 PMC: 7729053. DOI: 10.1098/rsif.2020.0776.

Selection of Immature Cat Oocytes with Brilliant Cresyl Blue Stain Improves In Vitro Embryo Production during Non-Breeding Season.

Piras A, Ariu F, Zedda M, Paramio M, Bogliolo L Animals (Basel). 2020; 10(9).

PMID: 32847086 PMC: 7552244. DOI: 10.3390/ani10091496.

References

Wang Q, Sun Q . Evaluation of oocyte quality: morphological, cellular and molecular predictors. Reprod Fertil Dev. 2007; 19(1):1-12. DOI: 10.1071/rd06103. View

Hammadeh M, Fischer-Hammadeh C, Ali K . Assisted hatching in assisted reproduction: a state of the art. J Assist Reprod Genet. 2010; 28(2):119-28. PMC: 3059528. DOI: 10.1007/s10815-010-9495-3. View

Alm H, Torner H, Lohrke B, Viergutz T, Ghoneim I, Kanitz W . Bovine blastocyst development rate in vitro is influenced by selection of oocytes by brillant cresyl blue staining before IVM as indicator for glucose-6-phosphate dehydrogenase activity. Theriogenology. 2005; 63(8):2194-205. DOI: 10.1016/j.theriogenology.2004.09.050. View

El Shourbagy S, Spikings E, Freitas M, St John J . Mitochondria directly influence fertilisation outcome in the pig. Reproduction. 2006; 131(2):233-45. DOI: 10.1530/rep.1.00551. View

Ghanem N, Holker M, Rings F, Jennen D, Tholen E, Sirard M . Alterations in transcript abundance of bovine oocytes recovered at growth and dominance phases of the first follicular wave. BMC Dev Biol. 2007; 7:90. PMC: 1976425. DOI: 10.1186/1471-213X-7-90. View

Koester M, Mohammadi-Sangcheshmeh A, Montag M, Rings F, Schimming T, Tesfaye D . Evaluation of bovine zona pellucida characteristics in polarized light as a prognostic marker for embryonic developmental potential. Reproduction. 2011; 141(6):779-87. DOI: 10.1530/REP-10-0471. View

Griffin J, Emery B, Huang I, Peterson C, Carrell D . Comparative analysis of follicle morphology and oocyte diameter in four mammalian species (mouse, hamster, pig, and human). J Exp Clin Assist Reprod. 2006; 3:2. PMC: 1413548. DOI: 10.1186/1743-1050-3-2. View

Rodriguez-Gonzalez E, Lopez-Bejar M, Velilla E, Paramio M . Selection of prepubertal goat oocytes using the brilliant cresyl blue test. Theriogenology. 2002; 57(5):1397-409. DOI: 10.1016/s0093-691x(02)00645-3. View

Pawlak P, Renska N, Pers-Kamczyc E, Warzych E, Lechniak D . The quality of porcine oocytes is affected by sexual maturity of the donor gilt. Reprod Biol. 2011; 11(1):1-18. DOI: 10.1016/s1642-431x(12)60060-6. View

10.

Rhodes T, McCoy T, Higdon 3rd H, Boone W . Factors affecting assisted reproductive technology (ART) pregnancy rates: a multivariate analysis. J Assist Reprod Genet. 2005; 22(9-10):335-46. DOI: 10.1007/s10815-005-6794-1. View

11.

Mota G, Pereira Batista R, Serapiao R, Cortes Boite M, Viana J, Torres C . Developmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue. Zygote. 2009; 18(3):209-16. DOI: 10.1017/S0967199409990219. View

12.

Rodriguez-Gonzalez E, Lopez-Bejar M, Izquierdo D, Paramio M . Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation. Reprod Nutr Dev. 2003; 43(2):179-87. DOI: 10.1051/rnd:2003012. View

13.

Pujol M, Lopez-Bejar M, Paramio M . Developmental competence of heifer oocytes selected using the brilliant cresyl blue (BCB) test. Theriogenology. 2003; 61(4):735-44. DOI: 10.1016/s0093-691x(03)00250-4. View

14.

Ebner T, Moser M, Sommergruber M, Tews G . Selection based on morphological assessment of oocytes and embryos at different stages of preimplantation development: a review. Hum Reprod Update. 2003; 9(3):251-62. DOI: 10.1093/humupd/dmg021. View

15.

Sirard M, Richard F, Blondin P, Robert C . Contribution of the oocyte to embryo quality. Theriogenology. 2005; 65(1):126-36. DOI: 10.1016/j.theriogenology.2005.09.020. View

16.

Borini A, Cattoli M, Bulletti C, Coticchio G . Clinical efficiency of oocyte and embryo cryopreservation. Ann N Y Acad Sci. 2008; 1127:49-58. DOI: 10.1196/annals.1434.012. View

17.

Wu Y, Liu Y, Zhou P, Lan G, Han D, Miao D . Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: a study using the mouse model. Cell Res. 2007; 17(8):722-31. DOI: 10.1038/cr.2007.66. View

18.

Spikings E, Alderson J, St John J . Regulated mitochondrial DNA replication during oocyte maturation is essential for successful porcine embryonic development. Biol Reprod. 2006; 76(2):327-35. DOI: 10.1095/biolreprod.106.054536. View

19.

Bettegowda A, Lee K, Smith G . Cytoplasmic and nuclear determinants of the maternal-to-embryonic transition. Reprod Fertil Dev. 2007; 20(1):45-53. DOI: 10.1071/rd07156. View

20.

Tiffin G, Rieger D, Betteridge K, Yadav B, King W . Glucose and glutamine metabolism in pre-attachment cattle embryos in relation to sex and stage of development. J Reprod Fertil. 1991; 93(1):125-32. DOI: 10.1530/jrf.0.0930125. View