Cadherin-9 Regulates Synapse-specific Differentiation in the Developing Hippocampus

Overview

Journal Neuron

Publisher Cell Press

Specialty Neurology

Date 2011 Aug 27

PMID 21867881

Citations 115

Authors

Megan E Williams

Scott A Wilke

Anthony Daggett

Elizabeth Davis

Stefanie Otto

Deepak Ravi

Beth Ripley

Eric A Bushong

Mark H Ellisman

Gerd Klein

Anirvan Ghosh

Affiliations

Soon will be listed here.

Abstract

Our understanding of mechanisms that regulate the differentiation of specific classes of synapses is limited. Here, we investigate the formation of synapses between hippocampal dentate gyrus (DG) neurons and their target CA3 neurons and find that DG neurons preferentially form synapses with CA3 rather than DG or CA1 neurons in culture, suggesting that specific interactions between DG and CA3 neurons drive synapse formation. Cadherin-9 is expressed selectively in DG and CA3 neurons, and downregulation of cadherin-9 in CA3 neurons leads to a selective decrease in the number and size of DG synapses onto CA3 neurons. In addition, loss of cadherin-9 from DG or CA3 neurons in vivo leads to striking defects in the formation and differentiation of the DG-CA3 mossy fiber synapse. These observations indicate that cadherin-9 bidirectionally regulates DG-CA3 synapse development and highlight the critical role of differentially expressed molecular cues in establishing specific connections in the mammalian brain.

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