Normal Human Pluripotent Stem Cell Lines Exhibit Pervasive Mosaic Aneuploidy

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2011 Aug 23

PMID 21857983

Citations 28

Authors

Suzanne E Peterson

Jurjen W Westra

Stevens K Rehen

Holly Young

Diane M Bushman

Christine M Paczkowski

Yun C Yung

Candace L Lynch

Ha T Tran

Kyle S Nickey

Yu-Chieh Wang

Louise C Laurent

Jeanne F Loring

Melissa K Carpenter

Jerold Chun

Affiliations

Soon will be listed here.

Abstract

Human pluripotent stem cell (hPSC) lines have been considered to be homogeneously euploid. Here we report that normal hPSC--including induced pluripotent--lines are karyotypic mosaics of euploid cells intermixed with many cells showing non-clonal aneuploidies as identified by chromosome counting, spectral karyotyping (SKY) and fluorescent in situ hybridization (FISH) of interphase/non-mitotic cells. This mosaic aneuploidy resembles that observed in progenitor cells of the developing brain and preimplantation embryos, suggesting that it is a normal, rather than pathological, feature of stem cell lines. The karyotypic heterogeneity generated by mosaic aneuploidy may contribute to the reported functional and phenotypic heterogeneity of hPSCs lines, as well as their therapeutic efficacy and safety following transplantation.

Citing Articles

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