Exploiting Co-linearity Among Grass Species to Map the Aegilops Ventricosa-derived Pch1 Eyespot Resistance in Wheat and Establish Its Relationship to Pch2

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2011 Aug 12

PMID 21833553

Citations 14

Authors

C Burt

P Nicholson

Affiliations

Soon will be listed here.

Abstract

Introgressions into wheat from related species have been widely used as a source of agronomically beneficial traits. One such example is the introduction of the potent eyespot resistance gene Pch1 from the wild relative Aegilops ventricosa onto chromosome 7DL of wheat. In common with genes carried on many other such introgressions, the use of Pch1 in commercial wheat varieties has been hindered by linkage drag with yield-limiting traits. Attempts to break this linkage have been frustrated by a lack of co-dominant PCR markers suitable for identifying heterozygotes in F(2) populations. We developed conserved orthologous sequence (COS) markers, utilising the Brachypodium distachyon (Brachypodium) genome sequence, to provide co-dominant markers in the Pch1 region. These were supplemented with previously developed sequence-tagged site (STS) markers and simple sequence repeat (SSR) markers. Markers were applied to a panel of varieties and to a BC(6) F(2) population, segregating between wheat and Ae. ventricosa over the distal portion of 7DL, to identify recombinants in the region of Pch1. By exploiting co-linearity between wheat chromosome 7D, Brachypodium chromosome 1, rice chromosome 6 and sorghum chromosome 10, Pch1 was located to an interval between the flanking markers Xwg7S and Xcos7-9. Furthermore candidate gene regions were identified in Brachypodium (364 Kb), rice (178 Kb) and sorghum (315 Kb) as a prelude to the map-based cloning of the gene. In addition, using homoeologue transferable markers, we obtained evidence that the eyespot resistances Pch1 and Pch2 on chromosomes 7D and 7A, respectively, are potentially homoeoloci. It is anticipated that the COS marker methodology could be used for the identification of recombinants in other introgressions into wheat from wild relatives. This would assist the mapping of genes of interest and the breaking of deleterious linkages to enable greater use of these introgressions in commercial varieties.

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References

Ouyang S, Zhu W, Hamilton J, Lin H, Campbell M, Childs K . The TIGR Rice Genome Annotation Resource: improvements and new features. Nucleic Acids Res. 2006; 35(Database issue):D883-7. PMC: 1751532. DOI: 10.1093/nar/gkl976. View

Paterson A, Bowers J, Bruggmann R, Dubchak I, Grimwood J, Gundlach H . The Sorghum bicolor genome and the diversification of grasses. Nature. 2009; 457(7229):551-6. DOI: 10.1038/nature07723. View

Song Q, Fickus E, Cregan P . Characterization of trinucleotide SSR motifs in wheat. Theor Appl Genet. 2003; 104(2-3):286-293. DOI: 10.1007/s001220100698. View

Krattinger S, Lagudah E, Spielmeyer W, Singh R, Huerta-Espino J, McFadden H . A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science. 2009; 323(5919):1360-3. DOI: 10.1126/science.1166453. View

Roder M, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P . A microsatellite map of wheat. Genetics. 1998; 149(4):2007-23. PMC: 1460256. DOI: 10.1093/genetics/149.4.2007. View

King J, Armstead I, Donnison I, Harper J, Roberts L, Thomas H . Introgression mapping in the grasses. Chromosome Res. 2007; 15(1):105-13. DOI: 10.1007/s10577-006-1103-0. View

McMillin D, Allan R, Roberts D . Association of an isozyme locus and strawbreaker foot rot resistance derived from Aegilops ventricosa in wheat. Theor Appl Genet. 2013; 72(6):743-7. DOI: 10.1007/BF00266539. View

Buschges R, Hollricher K, Panstruga R, Simons G, Wolter M, Frijters A . The barley Mlo gene: a novel control element of plant pathogen resistance. Cell. 1997; 88(5):695-705. DOI: 10.1016/s0092-8674(00)81912-1. View

. The map-based sequence of the rice genome. Nature. 2005; 436(7052):793-800. DOI: 10.1038/nature03895. View

10.

Ori N, Eshed Y, Paran I, Presting G, Aviv D, Tanksley S . The I2C family from the wilt disease resistance locus I2 belongs to the nucleotide binding, leucine-rich repeat superfamily of plant resistance genes. Plant Cell. 1997; 9(4):521-32. PMC: 156936. DOI: 10.1105/tpc.9.4.521. View

11.

Mena M, Doussinault G, Lopez-Brana I, Aguaded S, Garcia-Olmedo F, Delibes A . Eyespot resistance gene Pch-1 in H-93 wheat lines. Evidence of linkage to markers of chromosome group 7 and resolution from the endopeptidase locus Ep-D1b. Theor Appl Genet. 2013; 83(8):1044-7. DOI: 10.1007/BF00232970. View

12.

Somers D, Isaac P, Edwards K . A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet. 2004; 109(6):1105-14. DOI: 10.1007/s00122-004-1740-7. View

13.

Pena R, Murray T, Jones S . Identification of an RFLP interval containing Pch2 on chromosome 7AL in wheat. Genome. 1997; 40(2):249-52. DOI: 10.1139/g97-035. View

14.

Voigt C, Schafer W, Salomon S . A comprehensive view on organ-specific callose synthesis in wheat (Triticum aestivum L.): glucan synthase-like gene expression, callose synthase activity, callose quantification and deposition. Plant Physiol Biochem. 2006; 44(4):242-7. DOI: 10.1016/j.plaphy.2006.05.001. View

15.

Garvin D, McKenzie N, Vogel J, Mockler T, Blankenheim Z, Wright J . An SSR-based genetic linkage map of the model grass Brachypodium distachyon. Genome. 2010; 53(1):1-13. DOI: 10.1139/g09-079. View

16.

Feuillet C, Langridge P, Waugh R . Cereal breeding takes a walk on the wild side. Trends Genet. 2007; 24(1):24-32. DOI: 10.1016/j.tig.2007.11.001. View

17.

Bossolini E, Wicker T, Knobel P, Keller B . Comparison of orthologous loci from small grass genomes Brachypodium and rice: implications for wheat genomics and grass genome annotation. Plant J. 2007; 49(4):704-17. DOI: 10.1111/j.1365-313X.2006.02991.x. View

18.

. Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature. 2010; 463(7282):763-8. DOI: 10.1038/nature08747. View

19.

Quraishi U, Abrouk M, Bolot S, Pont C, Throude M, Guilhot N . Genomics in cereals: from genome-wide conserved orthologous set (COS) sequences to candidate genes for trait dissection. Funct Integr Genomics. 2009; 9(4):473-84. DOI: 10.1007/s10142-009-0129-8. View

20.

Leonard J, Watson C, Carter A, Hansen J, Zemetra R, Santra D . Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1. Theor Appl Genet. 2007; 116(2):261-70. DOI: 10.1007/s00122-007-0664-4. View