Cloning and Expression of the Delayed-rectifier IsK Channel from Neonatal Rat Heart and Diethylstilbestrol-primed Rat Uterus

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Apr 1

PMID 2183220

Citations 34

Authors

K Folander

J S Smith

J Antanavage

C Bennett

R B Stein

R Swanson

Affiliations

Soon will be listed here.

Abstract

cDNAs encoding a delayed-rectifier-type K+ channel were cloned from both neonatal rat heart and ovariectomized, diethylstilbestrol-primed rat uterus by using the polymerase chain reaction. Both clones have nucleotide sequences identical to that encoding the rat kidney IsK channel [Takumi, T., Ohkubo, H. & Nakanishi, S. (1988) Science 242, 1042-1045] and encode a putative protein of 130 amino acids. Injection of RNA transcripts of the cDNAs into Xenopus oocytes resulted in the expression of a slowly activating, voltage-dependent K+ current. An antisense oligonucleotide, derived from the sequence of the clone, specifically inhibited the expression of the slow, outward current observed in cells injected with mRNAs isolated from the parent tissues (i.e., kidney, heart, and uterus), indicating that the cloned gene underlies the major K+ current expressed from RNA isolated from these tissues.

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