Morphometric Study on the Development of Magnocellular Neurons of the Supraoptic Nucleus Utilising Immunohistochemical Methods

Overview

Journal J Anat

Specialty Anatomy & Histology

Date 1990 Feb 1

PMID 2182586

Citations 3

Authors

M A LAZCANO

M L Bentura

A Toledano

Affiliations

Soon will be listed here.

Abstract

Vasopressin (VP)- and oxytocin (OXY)-producing neurons, components of the rat supraoptic nucleus, have been located with immunohistochemical methods, with the purpose of studying their morphofunctional characteristics during different phases of life (embryonic, juvenile, adult and senile). To carry out this study, an IBAS I (Kontron) computerised image analyser has been utilised. The hormone VP is first detected in the neuronal cytoplasm of 21 days old rat embryos and the hormone OXY appears in the neuronal cytoplasm later, in the newborn phase. The neuronal area with a positive reaction for the two neurohormones has been evaluated and it has been found that the quantity of reaction substance is proportional to the age. In the adult period, VP neurons possess a reaction area (198 microns 2) greater than that of OXY neurons (153 microns 2). In the SON, there are two neuronal shapes, fusiform and round; these shapes coexist in both hormonal types of neurons. Until Day 15 of postnatal development, the SON neurons are intermixed in the interior of the nucleus but in this period a neuronal redistribution is initiated. In the adult phase, OXY neurons are situated preferentially in the anterior, posterior and dorsal parts and VP neurons in the ventral and posterior parts, with both neurons being present in the intermediate part of the SON.

Citing Articles

Developmental regulation of a local positive autocontrol of supraoptic neurons.

Chevaleyre V, Dayanithi G, Moos F, Desarmenien M J Neurosci. 2000; 20(15):5813-9.

PMID: 10908622 PMC: 6772532.

Development of the hypothalamic vasopressin system and nephrons in Meriones shawi during ontogenesis.

Rabhi M, Ugrumov M, Goncharevskaya O, Bengelloun W, Calas A, Natochin Y Anat Embryol (Berl). 1996; 193(3):281-96.

PMID: 8881477 DOI: 10.1007/BF00198331.

Morphometric and neurosecretory changes in supraoptic neurons after D-amphetamine treatment.

Bentura M, LAZCANO M, Zahonero M, Toledano A J Neural Transm Gen Sect. 1992; 89(1-2):81-92.

PMID: 1418869 DOI: 10.1007/BF01245354.

References

Kawata M, Sano Y . Immunohistochemical identification of the oxytocin and vasopressin neurons in the hypothalamus of the monkey (Macaca fuscata). Anat Embryol (Berl). 1982; 165(2):151-67. DOI: 10.1007/BF00305474. View

Swanson L, Sawchenko P . Hypothalamic integration: organization of the paraventricular and supraoptic nuclei. Annu Rev Neurosci. 1983; 6:269-324. DOI: 10.1146/annurev.ne.06.030183.001413. View

Saphier D, Feldman S . Electrophysiologic evidence for neural connections between the paraventricular nucleus and neurons of the supraoptic nucleus in the rat. Exp Neurol. 1985; 89(1):289-94. DOI: 10.1016/0014-4886(85)90287-0. View

Day T, Randle J, Renaud L . Opposing alpha- and beta-adrenergic mechanisms mediate dose-dependent actions of noradrenaline on supraoptic vasopressin neurones in vivo. Brain Res. 1985; 358(1-2):171-9. DOI: 10.1016/0006-8993(85)90961-8. View

Laurent F, Hindelang C, Klein M, Stoeckel M, Felix J . Expression of the oxytocin and vasopressin genes in the rat hypothalamus during development: an in situ hybridization study. Brain Res Dev Brain Res. 1989; 46(1):145-54. DOI: 10.1016/0165-3806(89)90152-1. View

Eranko O . The cytology of the nucleus supraopticus of the rat. Ann Med Exp Biol Fenn. 1951; 29(2):158-73. View

Rinne U, KIVALO E . MATURATION OF HYPOTHALMIC NEUROSECRETION IN RAT, WITH SPECIAL REFERENCE TO THE NEUROSECRETORY MATERIAL PASSING INTO THE HYPOPHYSIAL PORTAL SYSTEM. Acta Neuroveg (Wien). 1964; 27:166-83. DOI: 10.1007/BF01226874. View

SOKOL H, VALTIN H . Evidence for the synthesis of oxytocin and vasopressin in separate neurons. Nature. 1967; 214(5085):314-6. DOI: 10.1038/214314a0. View

Donev S . Occurrence of the neurosecretory substance during the embryonic development of the guinea pig. Z Zellforsch Mikrosk Anat. 1970; 104(4):517-29. DOI: 10.1007/BF00335375. View

10.

IFFT J . An autoradiographic study of the time of final division of neurons in rat hypothalamic nuclei. J Comp Neurol. 1972; 144(2):193-204. DOI: 10.1002/cne.901440204. View

11.

VANDESANDE F, Dierickx K, de Mey J . Identification of separate vasopressin-neurophysin II and oxytocin-neurophysin I containing nerve fibres in the external region of the bovine median eminence. Cell Tissue Res. 1975; 158(4):509-16. DOI: 10.1007/BF00220216. View

12.

VANDESANDE F, Dierickx K . Identification of the vasopressin producing and of the oxytocin producing neurons in the hypothalamic magnocellular neurosecretroy system of the rat. Cell Tissue Res. 1975; 164(2):153-62. DOI: 10.1007/BF00218970. View

13.

Swaab D, Nijveldt F, Pool C . Distribution of oxytocin and vasopressin in the rat supraoptic and paraventricular nucleus. J Endocrinol. 1975; 67(3):461-2. DOI: 10.1677/joe.0.0670461. View

14.

Watkins W . Immunocytochemical study of the hypothalamo-neurohypophysial system. I. Localization of neurosecretory neurons containing neurophysin-I and neurophysin-II in the domestic pig. Cell Tissue Res. 1976; 175(2):165-81. DOI: 10.1007/BF00232077. View

15.

VANDESANDE F, Dierickx K . Immunocytochemical demonstration of separate vasotocinergic and mesotocinergic neurons in the amphibian hypothalamic magnocellular neurosecretory system. Cell Tissue Res. 1976; 175(3):289-96. DOI: 10.1007/BF00218707. View

16.

Goossens N, Dierickx K, VANDESANDE F . Immunocytochemical demonstration of thehypothalamo-hypophysial vasotocinergic system of Lampetra fluviatilis. Cell Tissue Res. 1977; 177(3):317-23. DOI: 10.1007/BF00220307. View

17.

Watkins W, Choy V . Immunocytochemical study of the hypothalamo-neurohypophysial system. III. Localization of oxytocin- and vasopressin-containing neurons in the pig hypothalamus. Cell Tissue Res. 1977; 180(4):491-503. DOI: 10.1007/BF00220170. View

18.

Antunes J, Zimmerman E . The hypothalamic magnocellular system of the rhesus monkey: an immunocytochemical study. J Comp Neurol. 1978; 181(3):539-65. DOI: 10.1002/cne.901810306. View

19.

Altman J, Bayer S . Development of the diencephalon in the rat. II. Correlation of the embryonic development of the hypothalamus with the time of origin of its neurons. J Comp Neurol. 1978; 182(4 Pt 2):973-93. DOI: 10.1002/cne.901820512. View

20.

Choy V, Watkins W . Maturation of the hypothalamo-neurohypophysial system. I. Localization of neurophysin, oxytocin and vasopressin in the hypothalamus and neural lobe of the developing rat brain. Cell Tissue Res. 1979; 197(2):325-36. DOI: 10.1007/BF00233923. View