Cell-surface Markers for the Isolation of Pancreatic Cell Types Derived from Human Embryonic Stem Cells

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2011 Aug 2

PMID 21804561

Citations 170

Authors

Olivia G Kelly

Man Yin Chan

Laura A Martinson

Kuniko Kadoya

Traci M Ostertag

Kelly G Ross

Mike Richardson

Melissa K Carpenter

Kevin A DAmour

Evert Kroon

Mark Moorman

Emmanuel E Baetge

Anne G Bang

Affiliations

Soon will be listed here.

Abstract

Using a flow cytometry-based screen of commercial antibodies, we have identified cell-surface markers for the separation of pancreatic cell types derived from human embryonic stem (hES) cells. We show enrichment of pancreatic endoderm cells using CD142 and of endocrine cells using CD200 and CD318. After transplantation into mice, enriched pancreatic endoderm cells give rise to all the pancreatic lineages, including functional insulin-producing cells, demonstrating that they are pancreatic progenitors. In contrast, implanted, enriched polyhormonal endocrine cells principally give rise to glucagon cells. These antibodies will aid investigations that use pancreatic cells generated from pluripotent stem cells to study diabetes and pancreas biology.

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